The effects of Nigella sativa ethanolic extract on proliferation and apoptosis of renal cell carcinoma ACHN cell line

زمینه و هدف: سیاه دانه (Nigella sativa) گیاهی ازتیره آلاله٬ علفی، یک ساله یا پایا است. ترکیبات این گیاه دارای خواص ضد سرطانی هستند. این مطالعه با هدف بررسی اثر سایتوتوکسیک و آپوپتوتیک عصاره الکلی سیاهدانه بر روی رشد سلول های سرطانی کلیه انسان رده ACHN و سلول های غیر سرطانی سالم رده L929 انجام شد. روش بررسی: در این مطالعه تجربی غلظت های مختلف عصاره الکلی سیاهدانه در محیط کشت، روی سلول های ACHN و L929 اثر داده شد. پس از 24، 48 و 72 ساعت، تغییرات مورفولوژیک ایجاد شده با میکروسکوپ معکوس ارزیابی گردید. با آزمون MTT اثر غلظت های عصاره بر درصد سلول های زنده هر دو رده سلولی در زمان‌های مذکور، از نظر کمی بررسی شد. بررسی میزان آپوپتوز با کیت فسفاتیدیل سرین با استفاده ار دستگاه فلوسیتومتری مشخص گردید. داده ها با استفاده از آنالیز آماری واریانس یک طرفه و آزمون تعقیبی Tukeyآنالیز شد. یافته ها: نتایج آزمون MTT نشان داد که غلظت های µg/ml 750 و بالاتر عصاره بر سلول های رده ACHN و غلظت های µg/ml 1250 و بالاتر عصاره بر روی سلول های رده L929 موجب کاهش معنی دار تعداد سلول های زنده ACHN و L929 می گردد (05/0P(P. نتیجه گیری: با توجه به نتایج این پژوهش عصاره الکلی سیاهدانه دارای اثرات آپوپتوتیک و سایتوتوکسیک بر سلول های سرطانی رده ACHN در مقایسه با سلول های سالم L929 است. لذا می توان عصاره الکلی سیاهدانه را به عنوان ترکیبی با اثرات سایتوتوکسیک روی سلول های سرطانی در درمان سرطان کلیه پیشنهاد کرد

Sensing of Alzheimer’s Disease and Multiple Sclerosis Using Nano-Bio Interfaces

It is well understood that patients with different diseases may have a variety of specific proteins (e.g., type, amount, and configuration) in their plasmas. When nanoparticles (NPs) are exposed to these plasmas, the resulting coronas may incorporate some of the disease-specific proteins. Using gold (Au) NPs with different surface properties and corona composition, we have developed a technology for the discrimination and detection of two neurodegenerative diseases, Alzheimer's disease (AD) and multiple sclerosis (MS). Applying a variety of techniques, including UV-visible spectra, colorimetric response analyses and liquid chromatography-tandem mass spectrometry, we found the corona-NP complexes, obtained from different human serums, had distinct protein composition, including some specific proteins that are known as AD and MS biomarkers. The colorimetric responses, analyzed by chemometrics and statistical methods, demonstrate promising capabilities of the technology to unambiguously identify and discriminate AD and MS. The developed colorimetric technology might enable a simple, inexpensive and rapid detection/discrimination of neurodegenerative diseases. KEYWORDS: Alzheimer’s disease; colorimetric technology; disease-specific protein corona; gold nanoparticles; multiple sclerosi

Resveratrol Suppresses Cardiac Renin Angiotensin System in the Late Phase of Left Ventricular Hypertrophy

Background and objectives: Resveratrol(3,5,4′-trihydroxy-trans-stilbene) is a natural polyphenole phytoalexin which exerts potential cardioprotective effects, but the cellular and molecular mechanisms responsible for these effects are still unknown. Cardiac renin angiotensin system (RAS) over-activation plays an important role in pathogenesis of left ventricular hypertrophy (LVH) progression. The aim of the study was to investigate the effects of resveratrol on the main components of RAS during early and late phase of myocardial hypertrophy. Methods: To consider the early and late phase of LVH, the rats were studied two and sixteen weeks after abdominal aorta banding without treatment (H2w and H16w groups, respectively) or with resveratrol (R) treatment. Intact animals served as control (Ctl). Arterial blood pressure was recorded by carotid cannulation. Angiotensin II (Ang II) level was measured using ELISA kit. Gene expression was evaluated by Real time RT-PCR technique. Cardiomyocyte size and fibrosis were assessed using haematoxylin/eosin and Masson trichrome staining, respectively Results: Results of this study showed that in H2w group AT1a mRNA level was increased significantly (pConclusion: Progression of LVH is accompanied by dynamic changes in RAS components expression in myocardial tissue. Resveratrol protects the heart against pressure overload-induced hypertrophy in part via RAS suppression

Sulfonated magnetic spirulina nanobiomaterial as a novel and environmentally friendly catalyst for the synthesis of dihydroquinazolin-4(1H)-ones in aqueous medium

Abstract Spirulina algae is an excellent candidate for catalyst preparation due to its reactive functional groups, cost-effectiveness, widespread commercial accessibility, and biodegradability. In this study, magnetized Spirulina was used for the synthesis of dihydroquinazolin-4(1H)-ones (DHQZs) as catalyst. Magnetized Spirulina was produced by CoFe2O4 and sulfonation method using chlorosulfonic acid to create the catalyst [CoFe2O4-Sp-SO3H]. It was affirmed by various techniques, including Fourier transform infrared (FT-IR), Vibrating sample magnetometry (VSM), Powder X-ray diffraction (XRD), Energy-dispersive X-ray spectroscopy (EDS), Thermogravimetric analysis (TGA), Transmission electron microscopy (TEM), Field emission scanning electron microscopy (FE-SEM), and elemental mapping techniques. DHQZs synthesis was accomplished through a concise one-pot, three-component reaction involving a range of diverse aldehydes, isatoic anhydride, and primary aromatic amine, within an aqueous medium. The method offers several advantages, including using green conditions, the generation of several new 2-furan-quinazolinone derivatives, chromatography-free purification, short reaction times, appropriate yield of product (75–96%), and catalyst recyclability. The proposed catalyst and water as solvent demonstrated a strong synergistic effect, leading to the prosperous synthesis of various novel dihydroquinazolinones at 60 °C. These numerous benefits make our approach highly attractive for academic research and industrial applications