Mannan-binding lectin in cerebrospinal fluid: a leptomeningeal protein

BACKGROUND: Mannan-binding lectin (MBL), a protein of the innate immune response is attracting increasing clinical interest, in particularly in relation to its deficiency. Due to its involvement in brain diseases, identifying the source of MBL in CSF is important. Analysis of cerebrospinal fluid (CSF) can provide data that discriminates between blood-, brain-, and leptomeninges-derived proteins. To detect the source of MBL in CSF we need to consider three variables: the molecular size-dependent concentration gradient between CSF and blood, the variation in transfer between blood and CSF, and the CSF MBL concentration correlation with the albumin CSF/serum quotient (QAlb), i.e., with CSF flow rate. METHODS: MBL was assayed in samples of CSF and serum with an ELISA, coated with anti MBL antibodies. Routine parameters such as albumin-, immunoglobulin- CSF/serum quotients, oligoclonal IgG and cell count were used to characterize the patient groups. Groups comprised firstly, control patients without organic brain disease with normal CSF and normal barrier function and secondly, patients without inflammatory diseases but with increased QAlb, i.e. with a blood CSF barrier dysfunction. RESULTS: MBL concentration in CSF was at least five-fold higher than expected for a molecular-size-dependent passage from blood. Secondly, in a QIgM/QAlb quotient diagram (Reibergram) 9/13 cases showed an intrathecal fraction in some cases over 80% of total CSF MBL concentration 3) The smaller inter-individual variation of MBL concentrations in CSF of the control group (CV = 66%) compared to the MBL concentrations in serum (CV = 146%) indicate an independent source of MBL in CSF. 4) The absolute MBL concentration in CSF increases with increasing QAlb. Among brain-derived proteins in CSF only the leptomeningeal proteins showed a (linear) increase with decreasing CSF flow rate, neuronal and glial proteins are invariant to changes of QAlb. CONCLUSIONS: MBL in CSF is predominantly brain-derived and all results pointed to the leptomeningeal cells as the source of the protein. The evaluation of this protein requires the interpretation of its absolute concentrations in CSF as a function of the albumin quotient, QAlb. This recognition of MBL in brain cells opens a new field of discussion about the function of the innate immune response in CNS in cases of acute and chronic neurological diseases

Flexible Control of Composite Parameters in Max/MSP

Fundamental to the development of musical or artistic creative work is the ability to transform raw materials. This ability implies the facility to master many facets of the material, and to shape it with plasticity. Computer music environments typically provide points of control to manipulate material by supplying parameters with controllable values. This capability to control the values of parameters is inadequate for many artistic endeavors, and does not reflect the analogous tools and methods of artists working with physical materials. Rather than viewing parameters in computer-based systems as single points of control, the authors posit that parameters must become more multifaceted and dynamic in order to serve the needs of artists. The authors propose an expanded notion of how to work with parameters in computer-centric environments for time-based art. A proposed partial solution to this problem is to give parameters additional properties that define their behavior. An example implementation of these ideas is presented in Jamoma

Enabling Participants to Play Rhythmic Solos Within a Group via Auctions

The paper presents the interactive music system SoloJam, which allows a group of participants with little or no musical training to effectively play together in a ``band-like'' setting. It allows the participants to take turns playing solos made up of rhythmic pattern sequences. We specify the issue at hand for allowing such participation as being the requirement of decentralised coherent circulation of playing solos. This is to be realised by some form of intelligence within the devices used for participation. Here we take inspiration from the Economic Sciences, and propose this intelligence to take the form of making devices possessing the capability of evaluating their utility of playing the next solo, the capability of holding auctions, and of bidding within them. We show that holding auctions and bidding within them enables decentralisation of co-ordinating solo circulation, and a properly designed utility function enables coherence in the musical output. The approach helps achieve decentralised coherent circulation with artificial agents simulating human participants. The effectiveness of the approach is further supported when human users participate. As a result, the approach is shown to be effective at enabling participants with little or no musical training to play together in SoloJam

Engineering novel complement activity into a pulmonary surfactant protein

Complement neutralizes invading pathogens, stimulates inflammatory and adaptive immune responses, and targets non- or altered-self structures for clearance. In the classical and lectin activation pathways, it is initiated when complexes composed of separate recognition and activation subcomponents bind to a pathogen surface. Despite its apparent complexity, recognition-mediated activation has evolved independently in three separate protein families, C1q, mannose-binding lectins (MBLs), and serum ficolins. Although unrelated, all have bouquet-like architectures and associate with complement-specific serine proteases: MBLs and ficolins with MBL-associated serine protease-2 (MASP-2) and C1q with C1r and C1s. To examine the structural requirements for complement activation, we have created a number of novel recombinant rat MBLs in which the position and orientation of the MASP-binding sites have been changed. We have also engineered MASP binding into a pulmonary surfactant protein (SP-A), which has the same domain structure and architecture as MBL but lacks any intrinsic complement activity. The data reveal that complement activity is remarkably tolerant to changes in the size and orientation of the collagenous stalks of MBL, implying considerable rotational and conformational flexibility in unbound MBL. Furthermore, novel complement activity is introduced concurrently with MASP binding in SP-A but is uncontrolled and occurs even in the absence of a carbohydrate target. Thus, the active rather than the zymogen state is default in lectin·MASP complexes and must be inhibited through additional regions in circulating MBLs until triggered by pathogen recognition

Developing the Dance Jockey system for musical interaction with the Xsens MVN suit

In this paper we present the Dance Jockey System, a system developed for using a full body inertial motion capture suit (Xsens MVN) in music/dance performances. We present different strategies for extracting relevant postures and actions from the continuous data, and how these postures and actions can be used to control sonic and musical features. The system has been used in several public performances, and we believe it has great potential for further exploration. However, to overcome the current practical and technical challenges when working with the system, it is important to further refine tools and software in order to facilitate making of new performance pieces. Proceedings of the 12th International Conference on New Interfaces for Musical Expression. University of Michigan Press 2012 ISBN 978-0-9855720-1-3

Differential Role of the Lectin Pathway of Complement Activation in Susceptibility to Neonatal Sepsis

Background. The incidence of bacterial sepsis during the neonatal period is high. Mannan-binding lectin (MBL), L-ficolin, and H-ficolin recognize microorganisms and activate the complement system viaMBL-associated serine proteases (MASPs). This study investigated whether cord blood concentrations of the lectin pathway proteins are associated with neonatal sepsis. >Methods. This was a case-control study including 47 infants with culture-proven sepsis during the first month of life and 94 matched controls. MBL, L-ficolin, H-ficolin, MASP-2, and MASP-3 levels were measured in cord blood with use of enzyme-linked immunosorbent assay and time-resolved immunofluorometric assay. Multivariate logistic regression was performed. Results. Infants with gram-positive sepsis had significantly lower H-ficolin cord blood concentrations than controls (multivariate odds ratio [OR], 4.00; 95% confidence interval [CI], 1.51-10.56; P=.005), whereas infants with gram-negative sepsis had lower MBL cord blood concentrations (OR, 2.99; 95% CI, 0.86-10.33; P=.084). When excluding patients with postoperative sepsis, multivariate analysis confirmed that low H-ficolin was associated with a significantly higher risk of gram-positive sepsis (OR, 3.71; 95% CI, 1.26-10.92; P=.017) and late-onset sepsis (OR, 3.14; 95% CI, 1.07-9.21; P=.037). In contrast, low MBL was associated with a significantly higher risk of gram-negative sepsis (OR, 4.39; 95% CI, 1.10-17.45; P=.036) and early-onset sepsis (OR, 3.87; 95% CI, 1.05-14.29; P=.042). The concentrations of all the lectin pathway proteins increased with gestational age (P<.01). Conclusions. These preliminary results indicate that low MBL concentrations are a susceptibility factor for gram-negative sepsis, and low H-ficolin concentrations indicate susceptibility to gram-positive sepsis. The decreased expression of lectin pathway proteins in neonates must be considered to be an additional form of neonatal immunodeficienc

From experimental music technology to clinical tool

Human body motion is integral to all parts of musical experience, from performance to perception. But how is it possible to study body motion in a systematic manner? This article presents a set of video-based visualisation techniques developed for the analysis of music-related body motion, including motion images, motion-history images and motiongrams. It includes examples of how these techniques have been used in studies of music and dance performances, and how they, quite unexpectedly, have become useful in laboratory experiments on attentiondeficit/ hyperactivity disorder (ADHD) and clinical studies of cerebral palsy (CP). Finally, it includes reflections regarding what music researchers can contribute to the study of human motion and behaviour in general