Acetylcholine Receptors and Concanavalin A-Binding Sites on Cultured Xenopus Muscle Cells: Electrophoresis, Diffusion, and Aggregation

Using digitally analyzed fluorescence videomicroscopy, we have examined the behavior of acetylcholine receptors and concanavalin A binding sites in response to externally applied electric fields. The distributions of these molecules on cultured Xenopus myoballs were used to test a simple model which assumes that electrophoresis and diffusion are the only important processes involved. The model describes the distribution of concanavalin A sites quite well over a fourfold range of electric field strengths; the results suggest an average diffusion constant of ~2.3 X 10^(-9) cm^2/s. At higher electric field strengths, the asymmetry seen is substantially less than that predicted by the model. Acetylcholine receptors subjected to electric fields show distributions substantially different from those predicted on the basis of simple electrophoresis and diffusion, and evidence a marked tendency to aggregate. Our results suggest that this aggregation is due to lateral migration of surface acetylcholine receptors, and is dependent on surface interactions, rather than the rearrangement of microfilaments or microtubules. The data are consistent with a diffusion-trap mechanism of receptor aggregation, and suggest that the event triggering receptor localization is a local increase in the concentration of acetylcholine receptors, or the electrophoretic concentration of some other molecular species. These observations suggest that, whatever mechanism(s) trigger initial clustering events in vivo, the accumulation of acetylcholine receptors can be substantially enhanced by passive, diffusion-mediated aggregation

Color-flavor locked strangelets

Finite lumps of color-flavor locked strange quark matter (CFL-strangelets) are significantly more stable than strangelets without color-flavor locking for wide ranges of parameters, increasing the likelihood of strangelet metastability, or even absolute stability beyond some minimum baryon number $A_{min}$. Whereas bulk CFL strange quark matter is electrically neutral, CFL-strangelets are positively charged, with $Z\approx 0.3 A^{2/3}$. This is quite different from ``ordinary'' strangelets and may provide a possible test of color-flavor locking if strangelets are detected in upcoming cosmic-ray space experiments.Comment: Version published in PRL Oct.22-issue. Minor changes onl