31,802 research outputs found
Universal relations between nongaussian fluctuations in heavy-ion collisions
We show that universality near a critical end point implies a characteristic
relation between third- and fourth-order baryon susceptibilities and
, resulting in a banana-shaped loop when is plotted as a
function of along a freeze-out line. Including the individual
enhancements of and near a critical point, these features may
be a consistent set of observations supporting the interpretation of baryon
fluctuations data as arising from criticality.Comment: 5 pages, 6 figures, revtex style; v2 as publishe
Two-dimensional small-world networks: navigation with local information
Navigation process is studied on a variant of the Watts-Strogatz small world
network model embedded on a square lattice. With probability , each vertex
sends out a long range link, and the probability of the other end of this link
falling on a vertex at lattice distance away decays as .
Vertices on the network have knowledge of only their nearest neighbors. In a
navigation process, messages are forwarded to a designated target. For and , a scaling relation is found between the average actual
path length and , where is the average length of the additional long
range links. Given , dynamic small world effect is observed, and the
behavior of the scaling function at large enough is obtained. At and 3, this kind of scaling breaks down, and different functions of the
average actual path length are obtained. For , the average actual
path length is nearly linear with network size.Comment: Accepted for publication in Phys. Rev.
Cloning and expression of first gene for biodegrading microcystins by Sphingopyxis sp. USTB-05
Harmful cyanobacterial blooms (HCBs) in natural waters are a growing environmental problem worldwide because microcystins (MCs) produced by cyanobacteria are potent hepatotoxins and tumor promoters. MCs are resistant against physical and chemical factors. Thus, biodegradation is the most efficient method for removing MCs, and a number of bacterial strains, especially genus _Sphingomonas_, have been isolated for biodegrading MCs. Although the pathway, enzyme, and gene for biodegrading MCs by _Sphingomonas sp._ have been widely identified recently, no gene concerned with the biodegradation of MCs has been successfully cloned and expressed. In this study, we show that the first and most important gene of mlrA, containing 1,008 bp nucleotides in length, in the biodegradation pathway of MCs by _Sphingopyxis sp._ USTB-05, which encodes an enzyme MlrA containing 336 amino acid residues, is firstly cloned and expressed in _E. coli_ DH5α, with a cloning vector of pGEM-T easy and an expression vector of pGEX-4T-1. The encoded and expressed enzyme MlrA is responsible for cleaving the target peptide bond between 3-amino-9-methoxy-2,6,8-trimethyl-10-phenyl-deca-4,6-dienoic acid (Adda) and Arg in the cyclic structure of microcystin-RR (MC-RR)and microcystin-LR(MC-LR), two typical and toxic types of MCs. Linear MC-RR and MC-LR are produced as the first products. These findings are important in constructing a new genetic bacterial strain for the efficient removal of MCs from the important water supplies and resolving the controversy on the biodegradation pathway of different types of MCs by genus _Sphingomonas_
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