GUDMAP - An Online GenitoUrinary Resource

The GenitoUrinary Development Molecular Anatomy Project (GUDMAP) is a consortium of laboratories working to provide the scientific and medical community with gene expression data and tools to facilitate research (see "www.gudmap.org":http://www.gudmap.org). The data provided by GUDMAP includes large _in situ_ hybridization screens (wholemount and section) and expression microarray analysis of components of the developing mouse urogenital system (including laser-captured material and FACS-isolated cells from transgenic reporter mice). In addition, a high-resolution anatomy ontology has been developed by members of the GUDMAP consortium to describe the subcompartments of the developing murine genitourinary tract. 

The GUDMAP Database Development Team and Editorial Office - both based in Edinburgh - function to ensure submission, curation, storage and presentation of the data submitted by the GUDMAP consortium. Our collective aim is twofold: 1) to simplify the process of submission so that data is publically available as soon as it is produced; and 2) to organize this information in a database and ensure that the online interface is continuously available and easy to use. Thus far, we have developed a range of tools that help both the submitter and the end user. These include: an online annotation tool that simplifies _in situ_ data submission through an ontology-based graphical user interface; a database interface that allows users to browse and query expression data, and to filter data by organ system; a heat-map display of microarray data and analyses. Furthermore, the Edinburgh team has developed a GUDMAP Disease Database that queries associations between genes, genitourinary diseases, and renal/urinary and reproductive phenotypes. In collaboration with GUDMAP consortium members at the CCHMC (Cincinnati Children's Hospital Medical Center), the Disease Database is being extended to include mammalian phenotypes mapped to OMIM entries. 

By virtue of its impressive dataset and its ease of use we hope that the GUDMAP Website will continue to serve as a powerful resource for biologists, clinicians and bioinformaticians with an interest in the urogenital system

Book Reviews

Reviews of: 'Focus on Vocabulary,' by Paul Nation and Peter Yongqi Gu; 'Research in Applied Linguistics: Becoming a Discerning Consumer,' by Fred L. Perry, Jr.; and 'The Psychology of the Language Learner: Individual Differences in Second Language Acquisition,' by Zoltan Dornyei

The Smithsonian-Mason Semester for Conservation Studies: Advancing Innovative Ways to Teach the Practice of Conservation

The Smithsonian-Mason Semester for Conservation Studies is the cornerstone of an innovative partnership between George Mason University and the Smithsonian Institution, established to educate future conservation practitioners in an experiential framework. This 16-credit residential program addresses complex conservation imperatives within diverse disciplines, including the biological, physical, and social sciences. We aim to teach conservation as conservation is practiced, engaging students in real-world experiences. The semester is rooted in an integrative teaching design, focusing on dialogue and problem-solving using case studies in immersive field and laboratory experiences. Students learn from a large number of conservation practitioners and professionals, often up to 50 in one semester, enabling a collaborative atmosphere in which students develop a professional network. Class sizes are kept deliberately small so that students may engage in intensive mentoring with faculty. Since the beginning of the program in 2008, faculty have been using a pre- and post-semester learning gains assessment, including quantitative and qualitative questions, to gauge student understanding of the academic subject matter and their proficiency with professional skills. Data from these assessments will be compiled and reviewed to evaluate student learning gains of complex skills within the field of conservation and investigate long-term career development outcomes of alumni.</jats:p

Design and Implementation of a Mesocosm Experiment On The Environmental Consequences of Nearshore Dispersant Use

ABSTRACT In April 1998 the first full-scale oil spill experiment was run at the Coastal Oilspill Simulation System (COSS) Facility in Corpus Christi, Texas. The facility contains nine 110-foot long, eight-foot deep wave tanks for simulated nearshore or intertidal habitat experiments. Features include an adjustable 2-foot tidal range, variable flow rate, and random wave capability. Sediment can be added to create bottom habitat and to develop an intertidal “shoreline.” The project compared the ecological effects when oil is allowed to strand on the beach to the effects when dispersed oil is present in very shallow areas. Untreated and dispersed weathered Arabian Medium crude oil was released in three tanks each. Two other tanks served as controls. The fate and effects of the oil or dispersed oil were evaluated over ten days using caged marine species and detailed hydrocarbon chemistry. The tests were successfully completed, demonstrating the facility's impressive potential for similar experiments. Preliminary data analyses indicate that water column effects of dispersed oil were not significantly different from those of untreated oil, and the use of dispersant led to a clear reduction in shoreline accumulation of oil.</jats:p

Secretory phospholipase A2-IIa is involved in prostate cancer progression and may potentially serve as a biomarker for prostate cancer

The majority of prostate cancers are indolent, whereas a significant portion of patients will require systemic treatment during the course of their disease. To date, only high Gleason scores are best associated with a poor prognosis in prostate cancer. No validated serum biomarker has been identified with prognostic power. Previous studies showed that secretory phospholipase A2-IIa (sPLA2-IIa) is overexpressed in almost all human prostate cancer specimens and its elevated levels are correlated with high tumor grade. Here, we found that sPLA2-IIa is overexpressed in androgen-independent prostate cancer LNCaP-AI cells relative to their androgen-dependent LNCaP cell counterparts. LNCaP-AI cells also secrete significantly higher levels of sPLA2-IIa. Blocking sPLA2-IIa function compromises androgen-independent cell growth. Inhibition of the ligand-induced signaling output of the HER network, by blocking PI3K-Akt signaling and the nuclear factor-kappaB (NF-κB)-mediated pathway, compromises both sPLA2-IIa protein expression and secretion, as a result of downregulation of sPLA2-IIa promoter activity. More importantly, we demonstrated elevated serum sPLA2-IIa levels in prostate cancer patients. High serum sPLA2-IIa levels are associated significantly with high Gleason score and advanced disease stage. Increased sPLA2-IIa expression was confirmed in prostate cancer cells, but not in normal epithelium and stroma by immunohistochemistry analysis. We showed that elevated signaling of the HER/HER2-PI3K-Akt-NF-κB pathway contributes to sPLA2-IIa overexpression and secretion by prostate cancer cells. Given that sPLA2-IIa overexpression is associated with prostate development and progression, serum sPLA2-IIa may serve as a prognostic biomarker for prostate cancer and a potential surrogate prostate biomarker indicative of tumor burden