The VirS/VirR two-component system regulates the anaerobic cytotoxicity, intestinal pathogenicity, and enterotoxemic lethality of Clostridium perfringens type C isolate CN3685.

Clostridium perfringens vegetative cells cause both histotoxic infections (e.g., gas gangrene) and diseases originating in the intestines (e.g., hemorrhagic necrotizing enteritis or lethal enterotoxemia). Despite their medical and veterinary importance, the molecular pathogenicity of C. perfringens vegetative cells causing diseases of intestinal origin remains poorly understood. However, C. perfringens beta toxin (CPB) was recently shown to be important when vegetative cells of C. perfringens type C strain CN3685 induce hemorrhagic necrotizing enteritis and lethal enterotoxemia. Additionally, the VirS/VirR two-component regulatory system was found to control CPB production by CN3685 vegetative cells during aerobic infection of cultured enterocyte-like Caco-2 cells. Using an isogenic virR null mutant, the current study now reports that the VirS/VirR system also regulates CN3685 cytotoxicity during infection of Caco-2 cells under anaerobic conditions, as found in the intestines. More importantly, the virR mutant lost the ability to cause hemorrhagic necrotic enteritis in rabbit small intestinal loops. Western blot analyses demonstrated that the VirS/VirR system mediates necrotizing enteritis, at least in part, by controlling in vivo CPB production. In addition, vegetative cells of the isogenic virR null mutant were, relative to wild-type vegetative cells, strongly attenuated in their lethality in a mouse enterotoxemia model. Collectively, these results identify the first regulator of in vivo pathogenicity for C. perfringens vegetative cells causing disease originating in the complex intestinal environment. Since VirS/VirR also mediates histotoxic infections, this two-component regulatory system now assumes a global role in regulating a spectrum of infections caused by C. perfringens vegetative cells

Laboratorio Virtual de Física Cuántica

El laboratorio virtual de física cuántica simula algunas de las experiencias que los estudiantes deben realizar en la asignatura Laboratorio de Física Cuántica del tercer curso del Grado en Física. Inicialmente, comenzó con la simulación del efecto fotoeléctrico y difracción de electrones. Ahora dispone de algunas simulaciones más. Este laboratorio virtual está diseñado como una herramienta para hacer más accesibles y comprensibles las experiencias del mundo cuántico y para familiarizar al estudiante con los procedimientos de medida y principios básicos de las experiencias de física cuántica.The Quantum Physics Virtual Lab simulates some of the experiences that the students must face at the Quantum Physics Laboratory in the third year of studies in Physics. Initially, the project was making the simulation of the electron diffraction and photoelectric effect experiences. Now it has some other simulations. The Virtual Lab is thought as a training tool to make accessible and comprehensive the experience and to get the student familiar with the measurement procedure and basic principles of the experience that afterwards he will have to do at the real Lab

Performance analysis of feedback-free collision resolution NDMA protocol

To support communications of a large number of deployed devices while guaranteeing limited signaling load, low energy consumption, and high reliability, future cellular systems require efficient random access protocols. However, how to address the collision resolution at the receiver is still the main bottleneck of these protocols. The network-assisted diversity multiple access (NDMA) protocol solves the issue and attains the highest potential throughput at the cost of keeping devices active to acquire feedback and repeating transmissions until successful decoding. In contrast, another potential approach is the feedback-free NDMA (FF-NDMA) protocol, in which devices do repeat packets in a pre-defined number of consecutive time slots without waiting for feedback associated with repetitions. Here, we investigate the FF-NDMA protocol from a cellular network perspective in order to elucidate under what circumstances this scheme is more energy efficient than NDMA. We characterize analytically the FF-NDMA protocol along with the multipacket reception model and a finite Markov chain. Analytic expressions for throughput, delay, capture probability, energy, and energy efficiency are derived. Then, clues for system design are established according to the different trade-offs studied. Simulation results show that FF-NDMA is more energy efficient than classical NDMA and HARQ-NDMA at low signal-to-noise ratio (SNR) and at medium SNR when the load increases.Peer ReviewedPostprint (published version

Mat-city: plan de Kenzo Tange para un mundo flotante

Peer Reviewe

Entanglement in a first-order quantum phase transition

4 págs.; 3 figs.; PACS number(s): 03.65.Ud, 03.67.Mn, 73.43.NqThe entanglement properties of the ground state for a system of spins half embedded in a magnetic field were investigated. A first-order transition was obtained at zero field. It was found that two-spin entanglement displays a jump at the transition point. It was shown that the symmetries of the Hamiltonian allow to simplify the diagnolization.Peer Reviewe

Study of the Influence of Helical Milling Parameters on the Quality of Holes in the UNS R56400 Alloy

Helical milling has been positioned as an alternative to conventional drilling, where the advantages it offers make it very attractive for use on difficult-to-machine alloys such as the titanium alloy UNS R56400. However, the correlation between the indicator of hole quality and the kinematic parameters has rarely been studied. The kinematics are what bring most advantages and that is why it is necessary to know their influence. In this aspect, there are different focuses of problems associated with the complexity of the process kinematics, which makes it necessary to undertake a deeper analysis of the process and to carry out a preliminary study. To address this problem, a DOE (Design of Experiments) is proposed to identify the sensitivity and the main trends of the properties that define the quality holes with respect to the kinematic parameters. At the same time, a nomenclature is proposed to unify and avoid misinterpretations. This study has allowed us to obtain conclusive results that offer very relevant information for future researc

Self-Calibration Methods for Uncontrolled Environments in Sensor Networks: A Reference Survey

Growing progress in sensor technology has constantly expanded the number and range of low-cost, small, and portable sensors on the market, increasing the number and type of physical phenomena that can be measured with wirelessly connected sensors. Large-scale deployments of wireless sensor networks (WSN) involving hundreds or thousands of devices and limited budgets often constrain the choice of sensing hardware, which generally has reduced accuracy, precision, and reliability. Therefore, it is challenging to achieve good data quality and maintain error-free measurements during the whole system lifetime. Self-calibration or recalibration in ad hoc sensor networks to preserve data quality is essential, yet challenging, for several reasons, such as the existence of random noise and the absence of suitable general models. Calibration performed in the field, without accurate and controlled instrumentation, is said to be in an uncontrolled environment. This paper provides current and fundamental self-calibration approaches and models for wireless sensor networks in uncontrolled environments

Entanglement in a first order quantum phase transition

The phase diagram of spins 1/2 embedded in a magnetic field mutually interacting antiferromagnetically is determined. Contrary to the ferromagnetic case where a second order quantum phase transition occurs, a first order transition is obtained at zero field. The spectrum is computed for a large number of spins and allows one to study the ground state entanglement properties which displays a jump of its concurrence at the critical point.Comment: 4 pages, 3 EPS figure