Engagement Effects of Player Rating System-Based Matchmaking for Level Ordering in Human Computation Games

Human computation games lack established ways of balancing the difficulty of tasks or levels served to players, potentially contributing to their low engagement rates. Traditional player rating systems have been suggested as a potential solution: using them to rate both players and tasks could estimate player skill and task difficulty and fuel player-task matchmaking. However, neither the effect of difficulty balancing on engagement in human computation games nor the use of player rating systems for this purpose has been empirically tested. We therefore examined the engagement effects of using the Glicko-2 player rating system to order tasks in the human computation game Paradox. An online experiment (n=294) found that both matchmaking-based and pure difficulty-based ordering of tasks led to significantly more attempted and completed levels than random ordering. Additionally, both matchmaking and random ordering led to significantly more di cult tasks being completed than pure difficulty-based ordering. We conclude that poor balancing contributes to poor engagement in human computation games, and that player rating system-based difficulty rating may be a viable and efficient way of improving both

The Ability of ChatGPT to Aid in the Rapid Development of Inoculation Message Treatments: A Case Study and Recommendations

One of the most widely known types of AI technologies in recent years is ChatGPT. ChatGPT is a state-of-the-art language model that has revolutionized natural language processing by generating human-like text with context and coherence, enabling new possibilities for human-AI interaction (Brown et al., 2020). This case study reports on a 10-week conversational AI training initiative to assess whether ChatGPT4 can (a) identify the structural elements and features of conventional inoculation messages (i.e., forewarnings and preemptive refutations); (b) augment inoculation message design features (i.e., linguistic signatures, language, and length); (c) adapt messages for specific audiences (i.e., younger populations, experts); and finally, (d) independently replicate the inoculation message development process on a topic of its choosing. Twenty-one different inoculation messages previously used in published inoculation studies provided the instructional foundation for teaching ChatGPT. A combination of prompting techniques were used (i.e., sequential, active, iterative, and chain of thought prompts) to achieve the goals of the study. Using 29 different prompts we found a high degree of originality within the AI generated inoculation messages; however, structural weaknesses were prevalent regardless of originality. AI generated messages were more difficult to read and required an advanced education level to comprehend. The AI messages were not equivalent with the training exemplars, and, in general, contained higher percentages of complex wording. We also found that ChatGPT struggled with developing the explicit forewarning threat component and did not generate inoculation messages with more than two refutations. Several themes of metaphorical and figurative language were used by the conversational-AI. We describe and contextualize these findings and discuss considerations and recommendations for future study

Sequencing of 15 622 Gene-bearing BACs Clarifies the Gene-dense Regions of the Barley Genome

Barley (Hordeum vulgare L.) possesses a large and highly repetitive genome of 5.1 Gb that has hindered the development of a complete sequence. In 2012, the International Barley Sequencing Consortium released a resource integrating whole-genome shotgun sequences with a physical and genetic framework. However, because only 6278 bacterial artificial chromosome (BACs) in the physical map were sequenced, fine structure was limited. To gain access to the gene-containing portion of the barley genome at high resolution, we identified and sequenced 15 622 BACs representing the minimal tiling path of 72 052 physical-mapped gene-bearing BACs. This generated ~1.7 Gb of genomic sequence containing an estimated 2/3 of all Morex barley genes. Exploration of these sequenced BACs revealed that although distal ends of chromosomes contain most of the gene-enriched BACs and are characterized by high recombination rates, there are also gene-dense regions with suppressed recombination. We made use of published map-anchored sequence data from Aegilops tauschii to develop a synteny viewer between barley and the ancestor of the wheat D-genome. Except for some notable inversions, there is a high level of collinearity between the two species. The software HarvEST:Barley provides facile access to BAC sequences and their annotations, along with the barley–Ae. tauschii synteny viewer. These BAC sequences constitute a resource to improve the efficiency of marker development, map-based cloning, and comparative genomics in barley and related crops. Additional knowledge about regions of the barley genome that are gene-dense but low recombination is particularly relevant

Astrometry with the Keck-Interferometer: the ASTRA project and its science

The sensitivity and astrometry upgrade ASTRA of the Keck Interferometer is introduced. After a brief overview of the underlying interferometric principles, the technology and concepts of the upgrade are presented. The interferometric dual-field technology of ASTRA will provide the KI with the means to observe two objects simultaneously, and measure the distance between them with a precision eventually better than 100 uas. This astrometric functionality of ASTRA will add a unique observing tool to fields of astrophysical research as diverse as exo-planetary kinematics, binary astrometry, and the investigation of stars accelerated by the massive black hole in the center of the Milky Way as discussed in this contribution.Comment: 22 pages, 10 figures (low resolution), contribution to the summerschool "Astrometry and Imaging with the Very Large Telescope Interferometer", 2 - 13 June, 2008, Keszthely, Hungary, corrected authorlis

Proprioception and Tension Receptors in Crab Limbs: Student Laboratory Exercises

The primary purpose of these procedures is to demonstrate for teaching and research purposes how to record the activity of living primary sensory neurons responsible for proprioception as they are detecting joint position and movement, and muscle tension. Electrical activity from crustacean proprioceptors and tension receptors is recorded by basic neurophysiological instrumentation, and a transducer is used to simultaneously measure force that is generated by stimulating a motor nerve. In addition, we demonstrate how to stain the neurons for a quick assessment of their anatomical arrangement or for permanent fixation. Staining reveals anatomical organization that is representative of chordotonal organs in most crustaceans. Comparing the tension nerve responses to the proprioceptive responses is an effective teaching tool in determining how these sensory neurons are defined functionally and how the anatomy is correlated to the function. Three staining techniques are presented allowing researchers and instructors to choose a method that is ideal for their laboratory

Intracellular Recording, Sensory Field Mapping, and Culturing Identified Neurons in the Leech, \u3cem\u3eHirudo medicinalis\u3c/em\u3e

The freshwater leech, Hirudo medicinalis, is a versatile model organism that has been used to address scientific questions in the fields of neurophysiology, neuroethology, and developmental biology. The goal of this report is to consolidate experimental techniques from the leech system into a single article that will be of use to physiologists with expertise in other nervous system preparations, or to biology students with little or no electrophysiology experience. We demonstrate how to dissect the leech for recording intracellularly from identified neural circuits in the ganglion. Next we show how individual cells of known function can be removed from the ganglion to be cultured in a Petri dish, and how to record from those neurons in culture. Then we demonstrate how to prepare a patch of innervated skin to be used for mapping sensory or motor fields. These leech preparations are still widely used to address basic electrical properties of neural networks, behavior, synaptogenesis, and development. They are also an appropriate training module for neuroscience or physiology teaching laboratories

Electrochemical Signal Amplification for Pathogen Nucleic Acid Detection Utilizing a Cobalt-Based DNA-Binding Metallo-Intercalator

This paper reports the development of a highly sensitive and rapid electrochemical biosensor for the detection of pathogen nucleic acids. The primary objective was to enhance the detection sensitivity of DNA biosensors for pathogen nucleic acids commonly found in fresh and wastewaters, the food industry, and clinical samples. This enhanced sensitivity was achieved through the addition of a [Co(GA)2(aqphen)]Cl intercalating complex to increase the electrostatic field at the sensor surface/solution interface. Voltammetric and impedance-based detection techniques were employed to characterize the intercalation and redox-active properties of the compound. Additionally, non-Faradaic impedance and voltammetric methods were characterized as appropriate techniques for electrochemical detection. Implementing the [Co(GA)2(aqphen)]Cl intercalator led to increased voltammetric signal output using DPV, facilitating the rapid and sensitive detection of target DNA sequences. Notably, the [Co(GA)2(aqphen)]Cl permitted detection using non-Faradaic impedance in the absence of [Fe(CN)6]3-/4-. Characterization by cyclic voltammetric measurements revealed a surface-controlled redox mechanism and reversible electrochemistry of the compound intercalated with double-stranded DNA (dsDNA). Upon binding of 1 µM target DNA and 200 µM [Co(GA)2(aqphen)]Cl, a 2250% current peak increase was achieved. This increase enabled the sensitive detection of a target DNA sequence representative of E. coli DNA in buffer with an LOD of 67.5 pM, 100-fold more sensitive than the standard unlabeled assay while maintaining assay simplicity, low cost, and quick response. The use of [Co(GA)2(aqphen)]Cl among similar compounds in DNA biosensors offers a cost-effective and sensitive method for detecting waterborne pathogens such as E. coli. This approach could significantly improve environmental monitoring and pollution control by enabling more reliable and rapid monitoring of pathogens in water sources. Additionally, it has the potential to be of great use within the food industry and in point-of-care clinical settings

Effects of antiplatelet therapy on stroke risk by brain imaging features of intracerebral haemorrhage and cerebral small vessel diseases: subgroup analyses of the RESTART randomised, open-label trial

Background Findings from the RESTART trial suggest that starting antiplatelet therapy might reduce the risk of recurrent symptomatic intracerebral haemorrhage compared with avoiding antiplatelet therapy. Brain imaging features of intracerebral haemorrhage and cerebral small vessel diseases (such as cerebral microbleeds) are associated with greater risks of recurrent intracerebral haemorrhage. We did subgroup analyses of the RESTART trial to explore whether these brain imaging features modify the effects of antiplatelet therapy

Pharmacological Analysis of Dopamine Modulation in the \u3cem\u3eDrosophila melanogaster\u3c/em\u3e Larval Heart

Dopamine (DA) and other neurotransmitters affect nonneuronal tissues in insects by circulating in the hemolymph. In several organisms, DA has been shown to modulate distinct aspects of cardiac function but the signal transduction pathways that mediate dopaminergic effects on the heart are not well characterized. Here, we used a semiintact Drosophila melanogaster larva preparation and drugs targeting DA receptors and canonical second messenger pathways to identify signaling cascades that mediate the effect of DA on a myogenic heart. DA has a positive chronotropic effect that is mimicked by SKF38393 (type‐1 DA receptor agonist) and quinpirole (type‐2 DA receptor agonist). SCH23390 and spiperone (type‐1 and type‐2 DA receptor antagonists) are moderately effective at inhibiting DA\u27s effect. An adenylate cyclase inhibitor (SQ,22536) is also effective at blocking the stimulatory effect of DA but the drug has its own dose‐dependent effect. Activation of protein kinase C with a diacylglycerol analog has a stimulatory effect on heart rate (HR). These results suggest that (1) both DA receptor subtypes are expressed in third instar larva cardiac myocytes to increase HR in response to rising levels of DA in the hemolymph, and (2) canonical second messenger pathways modulate HR in D. melanogaster larvae. Having these disparate signaling cascades converge toward a common modulatory function appears redundant, but in the context of multiple cardioactive chemicals this redundancy is likely to increase the fidelity of signal transduction