Does litomosoides sigmodontis synthesize dimethylethanolamine from choline?

Juvenile female Litomosoides sigmodontis secrete a protein (Juv-p120) highly modified with dimethylethanolamine (DMAE). In an attempt to establish the source of this decoration worms were pulsed with [3H]-choline and [3H]-ethanolamine and the radio-isotope labelled products analysed. Both isotope labels were successfully taken up by the worms, as demonstrated by labelling of phospholipids with [3H]-choline, being predominantly incorporated into phosphatidylcholine and [3H]-ethanolamine into phosphatidylethanolamine. Isotope labelling of phosphatidylethanolamine was particularly striking with the worms taking up ~30 times as much labelled ethanolamine as choline. It was possible to detect faint labelling of Juv-p120 with [3H]-ethanolamine after prolonged exposure periods but, unlike the situation with the phospholipids, it was much more readily labelled with [3H]-choline. When pulsing with [3H]-ethanolamine it was also possible to detect isotope-labelled phosphatidylcholine, which may ultimately account for the low levels of labelling of Juv-p120. Overall our results raise the previously unconsidered but intriguing possibility that in L. sigmodontis, choline may be the precursor of DMAE

Charge-Mediated Localization of Conjugated Polythiophenes in Zwitterionic Model Cell Membranes.

The selective engineering of conjugated polyelectrolyte (CPE)-phospholipid interfaces is poised to play a key role in the design of advanced biomedical and biotechnological devices. Herein, we report a strategic study to investigate the relationship between the charge of the CPE side group and their association with zwitterionic phospholipid bilayers. The interaction of dipalmitoylphosphatidylcholine (DPPC) phospholipid vesicles with a series of poly(thiophene)s bearing zwitterionic, cationic, or anionic terminal groups (P3Zwit, P3TMAHT and P3Anionic, respectively) has been probed. Although all CPEs showed an affinity for the zwitterionic vesicles, the calculated partition coefficients determined using photoluminescence spectroscopy suggested preferential incorporation within the lipid bilayer in the order P3Zwit > P3Anionic ≫ P3TMAHT. The polarity probe Prodan was used to further qualify the position of the CPE inside the vesicle bilayers via Förster resonance energy transfer (FRET) studies. The varying proximity of the CPEs to Prodan was reflected in the Stern-Volmer quenching constants and decreased in the order P3Anionic > P3TMAHT ≫ P3Zwit. Dynamic light scattering measurements showed an increase in the hydrodynamic diameter of the DPPC vesicles upon addition of each poly(thiophene), but to the greatest extent for P3Anionic. Small-angle neutron scattering studies also revealed that P3Anionic specifically increased the thickness of the headgroup region of the phospholipid bilayer. Epifluorescence and atomic force microscopy imaging showed that P3TMAHT formed amorphous agglomerates on the vesicle surface, P3Zwit was buried throughout the bilayer, and P3Anionic formed a shell of protruding chains around the surface, which promoted vesicle fusion. The global data indicate three distinctive modes of interaction for the poly(thiophene)s within DPPC vesicles, whereby the nature of the association is ultimately controlled by the pendant charge group on each CPE chain. Our results suggest that charge-mediated self-assembly may provide a simple and effective route to design luminescent CPE probes capable of specific localization within phospholipid membranes

Making Mas: TruDynasty Carnival Takes Josephine Baker to the Caribbean Carnival

Jacqueline Taucar, in conversation with Thea and Dario Jackson, investigates the sculptural qualities of the Josephine Baker Mas for the Scotiabank Caribbean Carnival Festival in 2011. This article traces the conception, construction, and complexities of choreography for this carnivalesque reimagining of Baker in Paris of the twenties for a contemporary Canadian ambulant expression. This Queen Mas talks back to the objectification by Parisians and embodying Queen Mas as an instance of female empowerment

ER and HER2 expression are positively correlated in HER2 non-overexpressing breast cancer

PMCID: PMC3446380This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Sequential detection of multiple phase transitions in model biological membranes using a red-emitting conjugated polyelectrolyte.

The anionic conjugated polyelectrolyte, poly[3-(6-sulfothioatehexyl)thiophene] (P3Anionic), functions as a highly sensitive probe of membrane order, uniquely capable of sequentially detecting the three key phase transitions occurring within model phospholipid bilayers. The observed sensitivity is the result of charge-mediated, selective localisation of P3Anionic within the head-groups of the phospholipid bilayer

Unlocking Structure-Self-Assembly Relationships in Cationic Azobenzene Photosurfactants.

Azobenzene photosurfactants are light-responsive amphiphiles that have garnered significant attention for diverse applications including delivery and sorting systems, phase transfer catalysis, and foam drainage. The azobenzene chromophore changes both its polarity and conformation (trans-cis isomerization) in response to UV light, while the amphiphilic structure drives self-assembly. Detailed understanding of the inherent relationship between the molecular structure, physicochemical behavior, and micellar arrangement of azobenzene photosurfactants is critical to their usefulness. Here, we investigate the key structure-function-assembly relationships in the popular cationic alkylazobenzene trimethylammonium bromide (AzoTAB) family of photosurfactants. We show that subtle changes in the surfactant structure (alkyl tail, spacer length) can lead to large variations in the critical micelle concentration, particularly in response to light, as determined by surface tensiometry and dynamic light scattering. Small-angle neutron scattering studies also reveal the formation of more diverse micellar aggregate structures (ellipsoids, cylinders, spheres) than predicted based on simple packing parameters. The results suggest that whereas the azobenzene core resides in the effective hydrophobic segment in the trans-isomer, it forms part of the effective hydrophilic segment in the cis-isomer because of the dramatic conformational and polarity changes induced by photoisomerization. The extent of this shift in the hydrophobic-hydrophilic balance is determined by the separation between the azobenzene core and the polar head group in the molecular structure. Our findings show that judicious design of the AzoTAB structure enables selective tailoring of the surfactant properties in response to light, such that they can be exploited and controlled in a reliable fashion

Multimodal Control of Liquid Crystalline Mesophases from Surfactants with Photoswitchable Tails

Non-invasive manipulation of the hierarchical structure of functional materials is a key challenge in the advancement of optoelectronics, energy conversion and storage devices and drug delivery systems. Here, using a combination of small-angle X-ray scattering and polarised optical microscopy, we decipher the various structure/self- assembly relationships of neutral surfactants bearing photoswitchable tails, which self-organise into a rich variety of lyotropic liquid crystalline mesophases. Facile, multimodal control of the nanoscale morphology of these single-component systems is achieved through: i) molecular structure, via careful selection of the alkyl tail/ethylene oxide headgroup lengths; ii) concentration; iii) temperature; and iv) photoisomerisation. The nanoscale architectures range from the weakly concentrated hyperswollen lamellar phases, the more common lyotropic lamellar and hexagonal phases, to pure thermotropic liquid crystals; all of which are accessible at room temperature. Photoisomerisation with UV light leads to the reversible destruction of the liquid crystalline phase, which can be spatially controlled through the use of a mask. This extensive study demonstrates the versatility of neutral photosurfactants and paves the way for them to be investigated for new applications, such as photoresponsive templates or drug delivery systems.Isaac Newton Trust/University of Cambridge Early Career Support Scheme grant. Irish Research Council EU COST action MP120

A self-assembly toolbox for thiophene-based conjugated polyelectrolytes: surfactants, solvent and copolymerisation.

Targeted control of the aggregation, morphology and optical properties of conjugated polymers is critical for the development of high performance optoelectronic devices. Here, self-assembly approaches are used to strategically manipulate the order, conformation and spatial distribution of conjugated polymers in solution and subsequently prepared thin films. The supramolecular complex organisation of phosphonium-functionalised homo- (P3HTPMe3) and diblock (P3HT-b-P3HTPMe3) ionic conjugated polythiophenes upon solvent-mediation and co-assembly with oppositely charged surfactants is investigated. UV/Vis absorption and photoluminescence spectroscopies, small-angle neutron scattering (SANS), cryo-transmission electron microscopy (cryo-TEM) and atomic force microscopy (AFM) are used to probe the organisation and photophysical response of the aggregates formed. Subtle differences in the surfactant mole fraction and structure, as well as the solvent polarity, yield differences in the nature of the resultant homopolyelectrolyte-surfactant complexes. In contrast, only moderate structural transformations are observed for the amphiphilic diblock copolyelectrolyte, emphasising the structure "anchoring" effect of a neutral polymer block when amphiphilic copolymers are dissolved in polar solvents. These results highlight the versatility of self-assembly to access a range of nanomorphologies, which could be crucial for the design of the next generation of organic optoelectronic devices

Light-responsive self-assembly of a cationic azobenzene surfactant at high concentration.

The formation of high-concentration mesophases by a cationic azobenzene photosurfactant is described for the first time. Using a combination of polarised optical microscopy and small-angle X-ray scattering, optically anisotropic, self-assembled structures with long-range order are reported. The mesophases are disrupted or lost upon UV irradiation

Healthcare Staff Wellbeing, Burnout, and Patient Safety: A Systematic Review

Objective To determine whether there is an association between healthcare professionals’ wellbeing and burnout, with patient safety. Design Systematic research review. Data Sources PsychInfo (1806 to July 2015), Medline (1946 to July 2015), Embase (1947 to July 2015) and Scopus (1823 to July 2015) were searched, along with reference lists of eligible articles. Eligibility Criteria for Selecting Studies Quantitative, empirical studies that included i) either a measure of wellbeing or burnout, and ii) patient safety, in healthcare staff populations. Results Forty-six studies were identified. Sixteen out of the 27 studies that measured wellbeing found a significant correlation between poor wellbeing and worse patient safety, with six additional studies finding an association with some but not all scales used, and one study finding a significant association but in the opposite direction to the majority of studies. Twenty-one out of the 30 studies that measured burnout found a significant association between burnout and patient safety, whilst a further four studies found an association between one or more (but not all) subscales of the burnout measures employed, and patient safety. Conclusions Poor wellbeing and moderate to high levels of burnout are associated, in the majority of studies reviewed, with poor patient safety outcomes such as medical errors, however the lack of prospective studies reduces the ability to determine causality. Further prospective studies, research in primary care, conducted within the UK, and a clearer definition of healthcare staff wellbeing are needed. Implications This review illustrates the need for healthcare organisations to consider improving employees’ mental health as well as creating safer work environments when planning interventions to improve patient safety