Distinct mechanisms of Drosophila CRYPTOCHROME-mediated light-evoked membrane depolarization and in vivo clock resetting.

Drosophila CRYPTOCHROME (dCRY) mediates electrophysiological depolarization and circadian clock resetting in response to blue or ultraviolet (UV) light. These light-evoked biological responses operate at different timescales and possibly through different mechanisms. Whether electron transfer down a conserved chain of tryptophan residues underlies biological responses following dCRY light activation has been controversial. To examine these issues in in vivo and in ex vivo whole-brain preparations, we generated transgenic flies expressing tryptophan mutant dCRYs in the conserved electron transfer chain and then measured neuronal electrophysiological phototransduction and behavioral responses to light. Electrophysiological-evoked potential analysis shows that dCRY mediates UV and blue-light-evoked depolarizations that are long lasting, persisting for nearly a minute. Surprisingly, dCRY appears to mediate red-light-evoked depolarization in wild-type flies, absent in both cry-null flies, and following acute treatment with the flavin-specific inhibitor diphenyleneiodonium in wild-type flies. This suggests a previously unsuspected functional signaling role for a neutral semiquinone flavin state (FADH•) for dCRY. The W420 tryptophan residue located closest to the FAD-dCRY interaction site is critical for blue- and UV-light-evoked electrophysiological responses, while other tryptophan residues within electron transfer distance to W420 do not appear to be required for light-evoked electrophysiological responses. Mutation of the dCRY tryptophan residue W342, more distant from the FAD interaction site, mimics the cry-null behavioral light response to constant light exposure. These data indicate that light-evoked dCRY electrical depolarization and clock resetting are mediated by distinct mechanisms

Application of inertial instruments for DSN antenna pointing and tracking

The feasibility of using inertial instruments to determine the pointing attitude of the NASA Deep Space Network antennas is examined. The objective is to obtain 1 mdeg pointing knowledge in both blind pointing and tracking modes to facilitate operation of the Deep Space Network 70 m antennas at 32 GHz. A measurement system employing accelerometers, an inclinometer, and optical gyroscopes is proposed. The initial pointing attitude is established by determining the direction of the local gravity vector using the accelerometers and the inclinometer, and the Earth's spin axis using the gyroscopes. Pointing during long-term tracking is maintained by integrating the gyroscope rates and augmenting these measurements with knowledge of the local gravity vector. A minimum-variance estimator is used to combine measurements to obtain the antenna pointing attitude. A key feature of the algorithm is its ability to recalibrate accelerometer parameters during operation. A survey of available inertial instrument technologies is also given

Galvanic replacement of sub 20 nm Ag nanoparticles in organic media

Galvanic replacement is a versatile synthetic strategy for the synthesis of alloy and hollow nanostructures. The structural evolution of single crystalline and multiply twinned nanoparticles <20 nm in diameter and capped with oleylamine is systematically studied. Changes in chemical composition are dependent on the size and crystallinity of the parent nanoparticle. The effects of reaction temperature and rate of precursor addition are also investigated. Galvanic replacement of single crystal spherical and truncated cubic nanoparticles follows the same mechanism to form hollow octahedral nanoparticles, a mechanism which is not observed for galvanic replacement of Ag templates in aqueous systems. Multiply twinned nanoparticles can form nanorings or solid alloys by manipulating the reaction conditions. Oleylamine-capped Ag nanoparticles are highly adaptable templates to synthesize a range of hollow and alloy nanostructures with tuneable localised surface plasmon resonance

Conditional Hardness of Earth Mover Distance

The Earth Mover Distance (EMD) between two sets of points A, B subseteq R^d with |A| = |B| is the minimum total Euclidean distance of any perfect matching between A and B. One of its generalizations is asymmetric EMD, which is the minimum total Euclidean distance of any matching of size |A| between sets of points A,B subseteq R^d with |A| <= |B|. The problems of computing EMD and asymmetric EMD are well-studied and have many applications in computer science, some of which also ask for the EMD-optimal matching itself. Unfortunately, all known algorithms require at least quadratic time to compute EMD exactly. Approximation algorithms with nearly linear time complexity in n are known (even for finding approximately optimal matchings), but suffer from exponential dependence on the dimension. In this paper we show that significant improvements in exact and approximate algorithms for EMD would contradict conjectures in fine-grained complexity. In particular, we prove the following results: - Under the Orthogonal Vectors Conjecture, there is some c>0 such that EMD in Omega(c^{log^* n}) dimensions cannot be computed in truly subquadratic time. - Under the Hitting Set Conjecture, for every delta>0, no truly subquadratic time algorithm can find a (1 + 1/n^delta)-approximate EMD matching in omega(log n) dimensions. - Under the Hitting Set Conjecture, for every eta = 1/omega(log n), no truly subquadratic time algorithm can find a (1 + eta)-approximate asymmetric EMD matching in omega(log n) dimensions

Space Shuttle program communication and tracking systems interface analysis

The Space Shuttle Program Communications and Tracking Systems Interface Analysis began April 18, 1983. During this time, the shuttle communication and tracking systems began flight testing. Two areas of analysis documented were a result of observations made during flight tests. These analyses involved the Ku-band communication system. First, there was a detailed analysis of the interface between the solar max data format and the Ku-band communication system including the TDRSS ground station. The second analysis involving the Ku-band communication system was an analysis of the frequency lock loop of the Gunn oscillator used to generate the transmit frequency. The stability of the frequency lock loop was investigated and changes to the design were reviewed to alleviate the potential loss of data due the loop losing lock and entering the reacquisition mode. Other areas of investigation were the S-band antenna analysis and RF coverage analysis

Celebrity Coverage

Celebrity coverage is defined as mediated attention provided to well‐known individuals who through their state of fame become newsworthy. Coverage of celebrities ranges from stories about famous people in categories such as Hollywood, sports, politics, and music as well as celebrities who become famous through digital media. Celebrity journalism focuses on the process of becoming famous, known as the celebrification of individuals, and the influence celebrities have on politics, culture, and activism known as celebritization. Through an increasing interest in its content, celebrity coverage has turned into an economic good for media organizations. While celebrities continue to rely on mass media channels, digital media have changed not only celebrity culture, but also celebrity journalism

Addressing health literacy in patient decision aids

MethodsWe reviewed literature for evidence relevant to these two aims. When high-quality systematic reviews existed, we summarized their evidence. When reviews were unavailable, we conducted our own systematic reviews.ResultsAim 1: In an existing systematic review of PtDA trials, lower health literacy was associated with lower patient health knowledge (14 of 16 eligible studies). Fourteen studies reported practical design strategies to improve knowledge for lower health literacy patients. In our own systematic review, no studies reported on values clarity per se, but in 2 lower health literacy was related to higher decisional uncertainty and regret. Lower health literacy was associated with less desire for involvement in 3 studies, less question-asking in 2, and less patient-centered communication in 4 studies; its effects on other measures of patient involvement were mixed. Only one study assessed the effects of a health literacy intervention on outcomes; it showed that using video to improve the salience of health states reduced decisional uncertainty. Aim 2: In our review of 97 trials, only 3 PtDAs overtly addressed the needs of lower health literacy users. In 90% of trials, user health literacy and readability of the PtDA were not reported. However, increases in knowledge and informed choice were reported in those studies in which health literacy needs were addressed.ConclusionLower health literacy affects key decision-making outcomes, but few existing PtDAs have addressed the needs of lower health literacy users. The specific effects of PtDAs designed to mitigate the influence of low health literacy are unknown. More attention to the needs of patients with lower health literacy is indicated, to ensure that PtDAs are appropriate for lower as well as higher health literacy patients

Concorde noise-induced building vibrations John F. Kennedy International Airport

The outdoor and indoor noise levels resulting from aircraft flyovers and certain nonaircraft events were recorded at six home sites along with the associated vibration levels in the walls, windows, and floors of these test homes. Limited subjective tests conducted to examine the human detection and annoyance thresholds for building vibration and rattle caused by aircraft noise showed that both vibration and rattle were detected subjectively in several houses for some operations of both the Concorde and subsonic aircraft. Preliminary results indicate that the relationship between window vibration and aircraft noise is: (1) linear, with vibration levels being accurately predicted from OASPL levels measured near the window; (2) consistent from flyover to flyover for a given aircraft type under approach conditions; (3) no different for Concorde than for other conventional jet transports (in the case of window vibrations induced under approach power conditions); and (4) relatively high levels of window vibration measured during Concorde operations are due more to higher OASPL levels than to unique Concorde source characteristics

An information-bearing seed for nucleating algorithmic self-assembly

Self-assembly creates natural mineral, chemical, and biological structures of great complexity. Often, the same starting materials have the potential to form an infinite variety of distinct structures; information in a seed molecule can determine which form is grown as well as where and when. These phenomena can be exploited to program the growth of complex supramolecular structures, as demonstrated by the algorithmic self-assembly of DNA tiles. However, the lack of effective seeds has limited the reliability and yield of algorithmic crystals. Here, we present a programmable DNA origami seed that can display up to 32 distinct binding sites and demonstrate the use of seeds to nucleate three types of algorithmic crystals. In the simplest case, the starting materials are a set of tiles that can form crystalline ribbons of any width; the seed directs assembly of a chosen width with >90% yield. Increased structural diversity is obtained by using tiles that copy a binary string from layer to layer; the seed specifies the initial string and triggers growth under near-optimal conditions where the bit copying error rate is 17 kb of sequence information. In sum, this work demonstrates how DNA origami seeds enable the easy, high-yield, low-error-rate growth of algorithmic crystals as a route toward programmable bottom-up fabrication