Squamous Cell Carcinoma of Endometrium with Extensive Icthyosis Uteri

We report a rare case of squamous cell carcinoma of endometrium arising in icthyosis uteri in a 60 years old lady presenting with vaginal bleedin

P R A methods for studying the impact of technology adoption in crop and poultry enterprises in a rural village

Participatory Rural Appraisal (PRA) methods have of late been highly successful in bridging the gap between development functionaries and target groups for whom the development straties and efforts have been focused at. PRA describes a growing family of approaches and methods to enable local people to share, enhance and analyze their knowledge of life and conditions, to plan and to act. (Chambers, 1994)

Livelihood analysis using wealth ranking tool of PRA

The present study was undertaken in Maroorpatti village in Namakkal district of Tamilnadu. From the results o/the livelihood analysis conducted/or the three different categories a/wealth ranking, it may be pointed out that with respect to the size of land holdings the rich farmer had double the size a/the medium and as such large as 10 times that the poor farmer. The rich farmer had an incomparably large number o/cattle with respect to his counterparts. While the rich farmer received monthly income from livestock, the medium farmer, who was thus classified solely by his agricultural land holdings, always expected his agricultural field to boost his monthly income. Furthermore, from the expenditure pal1ern observed, the rich farmer's were inclined to save nearly 35% of their monthly income, while the poor farmers were always in need of 25% loan to meet their monthly needs. As a consequence, the crises analysis indicated that the poor farmer as well as the medium farmer usually takes a loan of 40% towards meeting any calamities faced in their agricultural and livestock sectors

Application of PRA technique for field problem identification

This paper deals with the application of participatory rural appraisal technique for identification of agricultural field problems of Maroorpatti village . The economic importance of problems was also worked out

Highly efficient transfection of human induced pluripotent stem cells using magnetic nanoparticles.

PurposeThe delivery of transgenes into human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes (hiPSC-CMs) represents an important tool in cardiac regeneration with potential for clinical applications. Gene transfection is more difficult, however, for hiPSCs and hiPSC-CMs than for somatic cells. Despite improvements in transfection and transduction, the efficiency, cytotoxicity, safety, and cost of these methods remain unsatisfactory. The objective of this study is to examine gene transfection in hiPSCs and hiPSC-CMs using magnetic nanoparticles (NPs).MethodsMagnetic NPs are unique transfection reagents that form complexes with nucleic acids by ionic interaction. The particles, loaded with nucleic acids, can be guided by a magnetic field to allow their concentration onto the surface of the cell membrane. Subsequent uptake of the loaded particles by the cells allows for high efficiency transfection of the cells with nucleic acids. We developed a new method using magnetic NPs to transfect hiPSCs and hiPSC-CMs. HiPSCs and hiPSC-CMs were cultured and analyzed using confocal microscopy, flow cytometry, and patch clamp recordings to quantify the transfection efficiency and cellular function.ResultsWe compared the transfection efficiency of hiPSCs with that of human embryonic kidney (HEK 293) cells. We observed that the average efficiency in hiPSCs was 43%±2% compared to 62%±4% in HEK 293 cells. Further analysis of the transfected hiPSCs showed that the differentiation of hiPSCs to hiPSC-CMs was not altered by NPs. Finally, robust transfection of hiPSC-CMs with an efficiency of 18%±2% was obtained.ConclusionThe difficult-to-transfect hiPSCs and hiPSC-CMs were efficiently transfected using magnetic NPs. Our study offers a novel approach for transfection of hiPSCs and hiPSC-CMs without the need for viral vector generation

Web Page Designing for Academic Libraries in Cyber Age

With the explosive growth of web today, libraries in their old concepts are no more in existence. A good and well designed and developed library web page is not only excellent media for publicizing the libra ry functions, activities, programs, resources and services but also helps to bring to the notice of users all significant information which they must know in developing and using their library in the process of time. In this respect, considerable thought should go to determining the kind of information and its presentation on the web. In view of above concepts an attempt is made in this paper to develop a model of web page designing for academic libraries basing on the existing available web pages

Studies on crossability between elite types of cardamom (Elettaria cardamomum Maton)

A 8 x 8 set of crosses including reciprocals were made between elite Malabar selections of cardamom (Elettaria cardamomum) namely, CCS-1 and RR-1 and six cardamom mosaic disease (katte) resistant lines (NKE-12, NKE-27, NKE-34, NK3,9, NKE-3 and NKE-19) to incorporate desirable characters in the hybrids. All the elite selections were compatible with each other; however, the degree of compatibility varied with the parents selected for hybridisation. The crossability was highest and significant in the cross NKE-19 x NKE-34 (92%) followed by NKE-12 x NKE-19 (77%) and NKE-12 x RR-1 (69%). The crossability in CCS-1 x RR-1, NKE-27 x CCS-1, NKE-12 x RR-1, NKE-9 x RR-1 and NKE-3 x RR-1 was high (>50%) indicating the scope for combining yield and disease resistance in these crosses. &nbsp

Studies on crossability between elite types of cardamom (Elettaria cardamomum Maton)

A 8 x 8 set of crosses including reciprocals were made between elite Malabar selections of cardamom (Elettaria cardamomum) namely, CCS-1 and RR-1 and six cardamom mosaic disease (katte) resistant lines (NKE-12, NKE-27, NKE-34, NK3,9, NKE-3 and NKE-19) to incorporate desirable characters in the hybrids. All the elite selections were compatible with each other; however, the degree of compatibility varied with the parents selected for hybridisation. The crossability was highest and significant in the cross NKE-19 x NKE-34 (92%) followed by NKE-12 x NKE-19 (77%) and NKE-12 x RR-1 (69%). The crossability in CCS-1 x RR-1, NKE-27 x CCS-1, NKE-12 x RR-1, NKE-9 x RR-1 and NKE-3 x RR-1 was high (>50%) indicating the scope for combining yield and disease resistance in these crosses. &nbsp

Studies on crossability between elite types of cardamom (Elettaria cardamomum Maton)

A 8 x 8 set of crosses including reciprocals were made between elite Malabar selections of cardamom (Elettaria cardamomum) namely, CCS-1 and RR-1 and six cardamom mosaic disease (katte) resistant lines (NKE-12, NKE-27, NKE-34, NK3,9, NKE-3 and NKE-19) to incorporate desirable characters in the hybrids. All the elite selections were compatible with each other; however, the degree of compatibility varied with the parents selected for hybridisation. The crossability was highest and significant in the cross NKE-19 x NKE-34 (92%) followed by NKE-12 x NKE-19 (77%) and NKE-12 x RR-1 (69%). The crossability in CCS-1 x RR-1, NKE-27 x CCS-1, NKE-12 x RR-1, NKE-9 x RR-1 and NKE-3 x RR-1 was high (>50%) indicating the scope for combining yield and disease resistance in these crosses. &nbsp