Renewable Natural Resources (RNR)Research Programme in Bhutan : An Overview

The objective of this paper is to give an overview of how the Renewable Natural Resources (RNR) research is being implemented in Bhutan. The RNR sector comprises mainly of agriculture, livestock and forestry sectors. Agriculture research in Bhutan initially started in early 1960s through the introduction of new crop and fruit varieties from Indian Agriculture Research Institutes. Thereafter, livestock and forestry research started in livestock farms and forestry research institutes, respectively. The first Agriculture Research Institute in Bhutan was established in 1982 which underwent various changes culminating into the present Council for RNR Research of Bhutan (CoRRB) in 2003. The Council is chaired by the Minister of Agriculture and the Director of CoRRB functions as the member Secretary of the Council. Four research centres with national and regional mandates located at strategic locations of the country were established to cater to the research and development needs of the farmers living in different agro-ecological zones. These centres are mandated to carry out research in the field of agriculture, horticulture, livestock, forestry and other cross cutting sectors of soils, plant protection, water management, meteorology, socio-economic studies. Apart from the Regional centres of research, there are sub centres where ever the area to be covered by the centre is large. The different departments also conduct research in consultation with CoRRB to avoid duplication of efforts. The main objectives of the RNR Research are to generate relevant technologies for the farming communities for ensuring food security and enhancement of rural income. These technologies are packaged as extension materials,news letters and journals for dissemination to farmers and all concerned stakeholders.Article信州大学農学部紀要. 46(1-2): 139-153 (2010)departmental bulletin pape

A Quick Energy Performance Simulation of Typical Bhutanese Residential Building Using Building Information Modelling

Authors would like to sincerely thank to Naresuan University for rendering all sort of help in this research and advising staffs for valuable feedback and suggestions. Choten Tshering, a scholarship student, also expresses his gratitude to His Majesty the King of Bhutan, and Naresuan University for providing him with a fully paid master’s degree scholarship.Bhutan is a tiny nation located in the Himalayan Mountain between India and China, with scarce natural resources and energy. So, this study aims to examine the energy performance of typical Bhutanese residential buildings by applying building information modelling. The typical Bhutanese residential building uses traditionally plastered brick walls and concrete block walls without insulation, resulting in greater heat loss from the structure. The heat loss from the building has a direct impact on the energy consumption of the building. Throughout most districts, the weather is cold from September to February, and mild with humid temperatures in the summer. The models have been constructed and analyzed using software called ‘Open Building Designer’ and ‘Energy Simulator’ under 3 scenarios: concrete block wall/concrete slab, brick wall/concrete slab, and stone masonry wall/timber flooring. The study was carried out on these three scenarios to ascertain the energy consumption, heat loss, and heat gain on the designed period. According to the simulation results, the Bhutanese residential building had a high heat loss from building but less heat intake from outside. Timber flooring and stone masonry wall are preferred in colder region as it can retain more heat if no insulation walls are used. The result also revealed that most of the heat from the structure was dissipated through the walls. Following that, a few types of insulated walls were used for simulation reasons, mostly to improve the building’s thermal performance. Heat loss from the structure was minimized up to 46–65% by adopting the insulated walls

Rabies virus strains circulating in Bhutan: implications for control.

We report a molecular epidemiological study of rabies virus strains circulating in animal populations in Bhutan, and investigate potential origins of these viruses. Twenty-three rabies virus isolates originating from dogs and other domestic animals were characterized by sequencing the partial nucleoprotein (N) gene (395 bp). Phylogenetic analysis was conducted and the Bhutanese isolates were compared with rabies viruses originating from other parts of the world. Phylogenetic analysis showed that Bhutanese isolates were highly similar and were closely related to Indian strains and South Asian Arctic–like–1 viruses. Our study suggests that the rabies viruses spreading in southern parts of Bhutan have originated from a common ancestor, perhaps from the Indian virus strain. Keywords: molecular epidemiology, rabies, Arctic–like virus, BhutanThailand Research Fund (grant No.DBG5180026

Rabies virus strains circulating in Bhutan: implications for control.

postprintWe report a molecular epidemiological study of rabies virus strains circulating in animal populations in Bhutan, and investigate potential origins of these viruses. Twenty-three rabies virus isolates originating from dogs and other domestic animals were characterized by sequencing the partial nucleoprotein (N) gene (395 bp). Phylogenetic analysis was conducted and the Bhutanese isolates were compared with rabies viruses originating from other parts of the world. Phylogenetic analysis showed that Bhutanese isolates were highly similar and were closely related to Indian strains and South Asian Arctic–like–1 viruses. Our study suggests that the rabies viruses spreading in southern parts of Bhutan have originated from a common ancestor, perhaps from the Indian virus strain. Keywords: molecular epidemiology, rabies, Arctic–like virus, BhutanThailand Research Fund (grant No.DBG5180026

Analysis of head and neck cancer scRNA-seq data identified PRDM6 promotes tumor progression by modulating immune gene expression

Head and neck squamous cell carcinoma (HNSCC) is a biologically aggressive and heterogeneous group of cancers with limited treatment options for patients who do not respond to standard therapies. While HPV-related HNSCCs tend to show better therapeutic outcomes, we still have limited understanding of the immune mechanisms underlying these cancers. Immune-responsive genes (IRGs) have emerged as critical factors in regulating both tumor progression and immune response. Recent advances in single-cell RNA sequencing (scRNA-seq) and the development of cell-type specific regulon inference tools, such as IRIS3, have provided new insights into the tumor immune microenvironment. In this study, we leveraged the IRIS3 platform to analyze scRNA-seq data from HNSCC patient samples, identifying novel transcription factor (TF)-IRG networks that contribute to tumor proliferation and immune escape. Specifically, we identified PRDM6, a histone methyltransferase, possesses the previously unknown role in promoting tumor cell proliferation by inducing IRG expression. We further demonstrated that HPV viral oncoproteins (E6/E7) up-regulate the PRDM6 expression, which associates PRDM6 with HPV-positive HNSCC

Yield of Brain MRI in Clinically Diagnosed Epilepsy in the Kingdom of Bhutan: A Prospective Study

Background: People with epilepsy (PWE) in low- and middle-income countries may not access the health resources that are considered optimal for epilepsy diagnosis. The diagnostic yield of magnetic resonance imaging (MRI) has not been well studied in these settings. Objectives: To report the diagnostic yield of brain MRI and identify clinical associations of abnormal MRI findings among PWE in a neurocysticercosis-endemic, resource-limited setting and to identify the proportion and putative structural brain causes of drug-resistant epilepsy. Methods: PWE were prospectively enrolled at the Jigme Dorji Wangchuck National Referral Hospital in Bhutan (2014-2015). Each participant completed clinical questionnaires and a 1.5-Tesla brain MRI. Each MRI was reviewed by at least 1 radiologist and neurologist in Bhutan and the United States. A working definition of drug-resistant epilepsy for resource-limited settings was given as (a) seizures for >1 year, (b) at least 1 seizure in the prior year, and (c) presently taking 2 or more antiepileptic drugs (AEDs). Logistic regression models were constructed to test the cross-sectional association of an abnormal brain MRI with clinical variables. Findings: A total of 217 participants (125 [57%] female; 54 [25%] neurocysticercosis (n = 26, 12%, including 1 child) and congenital/perinatal abnormalities (n = 29, 14%, including 14 children). The number of AEDs (odds ratio = .59, 'P' = .03) and duration of epilepsy (odds ratio = 1.11, 'P' = .02) were significantly associated with an abnormal MRI. Seizure in the prior month was associated with the presence of mesial temporal sclerosis (odds ratio = .47, 'P' = .01). A total of 25 (12%) participants met our definition of drug-resistant epilepsy, with mesial temporal sclerosis (n = 10), congenital malformations (n = 5), and neurocysticercosis (n = 4) being the more common findings. Conclusions: The prevalence of abnormalities on brain MRI for PWE in resource-limited settings is high as a result of a diffuse range of etiologies, most commonly mesial temporal sclerosis. Drug-resistant epilepsy accounted for 12% of the referral population in a conservative estimation

Mapping recommendations towards an Asian Code Against Cancer (ACAC) as part of the World Code Against Cancer Framework: an Asian National Cancer Centers Alliance (ANCCA) initiative

This paper outlines the process undertaken by Asian National Cancer Centers Alliance (ANCCA) members in working towards an Asian Code Against Cancer (ACAC). The process involves: (i) identification of the criteria for selecting the existing set of national recommendations for ACAC (ii) compilation of existing national codes or recommendations on cancer prevention (iii) reviewing the scientific evidence on cancer risk factors in Asia and (iv) establishment of one or more ACAC under the World Code Against Cancer Framework. A matrix of national codes or key recommendations against cancer in ANCCA member countries is presented. These include taking actions to prevent or control tobacco consumption, obesity, unhealthy diet, physical inactivity, alcohol consumption, exposure to occupational and environmental toxins; and to promote breastfeeding, vaccination against infectious agents and cancer screening. ANCCA will continue to serve as a supportive platform for collaboration, development, and advocacy of an ACAC jointly with the International Agency for Research on Cancer/World Health Organization (IARC/WHO)