A importância das ações extensionistas na avaliação e acompanhamento das dislipidemias em idosos

Anais do 35º Seminário de Extensão Universitária da Região Sul - Área temática: SaúdeO projeto “Contribuição laboratorial à saúde em uma instituição de longa permanência de idosos de Ponta Grossa – Paraná” da UEPG promove a integração da universidade com idosos de uma Instituição de Longa Permanência para Idosos (ILPI). As ações extensionistas, desenvolvidas por professores e alunos do curso de Farmácia, visam contribuir no acompanhamento clínico-laboratorial e na melhoria qualidade de vida desses idosos com graus variados de dependência para atividades de autocuidado. O convênio entre UEPG e ILPI garante a execução de exames sem custos para os idosos e a formação acadêmica dos alunos. No contexto do atendimento e do envelhecimento populacional abordou-se, neste trabalho, a importância da dislipidemia com fator de risco em idosos. Participaram do estudo 68 idosos, avaliados no período de setembro de 2016 a julho de 2017. Coletou-se sangue venoso para a determinação do perfil lipídico, segundo a V Diretriz de Dislipidemia e Prevenção da Aterosclerose. A dislipidemia esteve presente em 51% dos idosos. Seu controle tem sido apontado como medida preventiva primária de eventos cardiovasculares e o projeto extensionista tem sido essencial para o mapeamento do risco nos idosos. Por outro lado, a integração das ações: ensino, pesquisa e extensão, enriquece a formação de recursos humanos para o atendimento em saúde no cenário do envelheciment

Correlation between running anaerobic sprint test and anaerobic work capacity in soccer players

Running anaerobic sprint test (RAST) is theoretically an anaerobic measurement. Similarly, anaerobic work capacity (AWC), also evaluates anaerobic performance. However, their relationships as scientifically validated anaerobic tests are still controversial. This study evaluates associations between AWC and maximum velocity (Vmax), mean velocity (Vm) velocity, peak (PP), mean (PMab), absolute (PPab), and relative (PPre) power, fatigue index (IF), and peak lactatemia concentration ([la-]p). Nine sub-17 category soccer players from Ponta Grossa, PR, Brazil were submitted to six 35m efforts separated by 10s intervals to determine PPab, PMab, PPre, PMre, and IF (RAST). After three, five, and seven minutes blood samples were collected to determine [la-]p. Later, exhaustive tests were performed on a treadmill at 100%, 110%, and 120% VO2peak (previously determined). Points obtained in relation to velocity versus 1/tlim were linearly adjusted so that the linear coefficient corresponded to AWC. Possible associations between AWC and the RAST parameters were evaluated by the Pearson correlation test (P\u3c0.05). No significant correlations were seen between AWC and any of the powers, IF, and [la-]p. However, AWC significantly correlated with Vmax (r=0.79) and Vmed (r=0.83). We can therefore conclude that AWC did not correlate with traditional anaerobic parameters determined by RAST, but could predict Vmax and Vmed provided from this test

Yttrium-90 Radioembolization of Hepatic Metastases from Colorectal Cancer

Liver metastases from colorectal cancer (CRC) result in substantial morbidity and mortality. The primary treatment is systemic chemotherapy, and in selected patients, surgical resection; however, for patients who are not surgical candidates and/or fail systemic chemotherapy, liver-directed therapies are increasingly being utilized. Yttrium-90 (Y-90) microsphere therapy, also known as selective internal radiation therapy (SIRT) or radioembolization, has proven to be effective in terms of extending time to progression of disease and also providing survival benefit. This review focuses on the use of Y-90 microsphere therapy in the treatment of liver metastases from CRC, including a comprehensive review of published clinical trials and prospective studies conducted thus far. We review the methodology, outcomes, and side effects of Y-90 microsphere therapy for metastatic CRC

The implementation of a novel graphical python editor (STREMECODER) in a rodent discrimination apparatus

As the interest in neuroscience and the desire to perform behavioral tasks in a higher level of specificity and accuracy increases, the need to have tools and techniques to conduct experimentations in a low-cost automated manner is essential. Although such methods have been proposed previously by other researchers, they have presented their data and tools in a manner that would have been difficult to comprehend for non-programmers. In labs that do not have the accessibility to individuals who can understand the published procedures, it is very difficult for them to get started and manipulate the published procedures to their interests. Here in this paper, I discuss designing and implementing a computer program for a rodent odor discrimination apparatus using a novel graphical python programming editor named STREMECODER. The objective of this project is to create a program interface that helps the laboratory staff conduct their experiments in an automated, dynamic, and userfriendly manner

PRELIMINARY PHYTOCHEMICAL SCREENING AND ANTIMICROBIAL ACTIVITY OF DRIED FLOWERS OF ADENIUM OBESUM

Objective: To estimate the antimicrobial activity of methanolic extract of Adenium obesum (family Apocyanaceae) in association with phytochemical analysis. Methods: The methanolic extract of the flowers of Adenium obesum was prepared and investigated for phytochemical constituents using standard methods. Antimicrobial activity of methanolic extract Adenium obesum has been carried out against one gram-positive bacteria Staphylococcus aureus and one gram-negative bacteria Escherichia coli and the antifungal activity of the plant extract was evaluated on Candida albicans species. The testing was done by the disc diffusion method to visualize the zone of inhibition of methanolic extract was compared with that of standard drug i.e., Ciprofloxacin for antibacterial activity and Fluconazole for antifungal activity. The minimum inhibition concentration was determined by micro dilution method (cup-plate method). Results: The present investigation shows the phytochemical analysis confirmed the presence of alkaloids, saponin glycosides, flavonoids, phenols, proteins and amino acids, cardiac glycosides, steroids, tannin, and phenolic compounds. The anti-microbial activity of the methanolic extract of the plant showed significant results against all the three of the test organisms in compare to the standard drugs. Conclusion: The present study concluded that methanolic extract of the flowers of adenium obesum proved the existence of phytochemical constituents. The methanolic extract of the was found to possess promising antimicrobial activity when compared with the standards and it can be useful for the development of antibiotics

REAL TIME LEARNING LEVEL ASSESSMENT USING EYE TRACKING

E-Learning is emerging as a convenient and effective learning tool. However, the challenge with eLearning is the lack of effective tools to assess levels of learning. Ability to predict difficult content in real time enables eLearning systems to dynamically provide supplementary content to meet learners’ needs. Recent developments have made possible low-cost eye trackers, which enables a new class of applications based on eye response. In comparison to past attempts using bio-metrics in learning assessments, with eye tracking, we can have access to the exact stimulus that is causing the response. A key aspect of the proposed approach is the temporal analysis of eye response and stimulus (concept) that is causing the response. Variations in eye response to the same concept over time may be indicative of levels of learning. The proposed system analyses slide images to extract words and then maps eye response to those words. We propose an analytical model (refer figure 1) for predicting various levels of learning in real time and the model achieves a prediction accuracy of 70%

Characterisation of the biflavonoid hinokiflavone as a premRNA splicing modulator that inhibits SENP

We have identified the plant biflavonoid hinokiflavone as an inhibitor of splicing in vitro and modulator of alternative splicing in cells. Chemical synthesis confirms hinokiflavone is the active molecule. Hinokiflavone inhibits splicing in vitro by blocking spliceosome assembly, leading to accumulation of the A complex. Cells treated with hinokiflavone show altered subnuclear organization specifically of splicing factors required for A complex formation, which relocalize together with SUMO1 and SUMO2 into enlarged nuclear speckles. Hinokiflavone increases protein SUMOylation levels, both in in vitro splicing reactions and in cells. Hinokiflavone also inhibited a purified, E. coli expressed SUMO protease, SENP1, in vitro, indicating the increase in SUMOylated proteins results primarily from inhibition of de-SUMOylation. Using a quantitative proteomics assay we identified many SUMO2 sites whose levels increased in cells following hinokiflavone treatment, with the major targets including 6 proteins that are components of the U2 snRNP and required for A complex formation

A CAPITE AD CALCEM APPROACH IMPORTANCE IN CASE OF SYPHILIS

Background Nowadays, the patient full examination is challenging by limited time in different medical fields (Musellim B. et al, 2017). It can lead inappropriate diagnosis and treatment. Therefore, it is important to use patient examination gold Approach a capite ad calcem. Methods The medical documentation, pathology slides and data of laboratory results were reviewed in the context of upto-date medical literature. Results A 34-year-old white man presents with a complaint about itching on his legs and arms that follow him around four months. In objective examination of the overall status – without any deviation of the norm. In the local status, we found infiltrative, hyperaemic papules with yellowish crusted erosions on both legs and arms with well demarcated. On both legs we found varicose veins. Sexual history said that last sexual contact had been two years ago. The full blood test, infection tests (HIV, RPR, TPHA, HCV), microbiology and skin punch biopsy were done. Work diagnosis at the beginning: exacerbation of chronic eczema. Important to keep in mind, cutaneous features of secondary syphilis sometime can show as chronic dermatitis. The blood test result, total IgE showed without any deviation of the norm. RPR – positive (4+), TPHA (4+, 1:1280), HIV – negative, HCV – negative. Excreted from the skin: Staphylococcus aureus, Streptococcus pyogenes (beta haemolytic group A). Other sexual transmissive disease – negative. The skin punch biopsy was done from arm skin. At the pathophysiological conclusion said that: histological picture is possible in the case of Lyme disease. Lyme disease parameters are not determined due to treatment interruption at the patient‘s request. Conclusion A capite ad calcem approach is an essential part in dermatovenerology practice. It can help for doctor find not just seemingly the main reason for patient‘s complain but allow watch wider.Peer reviewe

Quantitative phase imaging of cells using White Light Diffraction Phase Microscopy

Quantitative phase imaging (QPI) techniques are very advantageous compared to the qualitative phase imaging techniques like bright field microscopy, phase contrast microscopy, differential interference contrast microscopy as the QPI techniques render phase information quantitatively whereas qualitative phase imaging techniques yield phase information qualitatively and cannot provide exact phase values. In QPI techniques, there is no need of exogenous contrast agents to stain or tag the specimen. White light diffraction phase microscopy (wDPM) is one of the QPI techniques which is used to quantify the phase information from the samples