Modelling And Simulation Of The Adsorption Of The Lipase From Geotrichum Sp. On Hydrophobic Interaction Columns

The Langmuir model fitted well the adsorption isotherms of lipase on the hydrophobic resin. The model parameters, Qm and kd, were affected by NaCl concentration: Qm increased from 31 to 80 U g-1 resin, and kd changed from 9.4 to 3 U ml-1. Column modelling and the simulation data were compared with the experimental data with good agreement. The highest achieved column efficiency was 71%. Notations: C: lipase concentration in solution (U ml-1); C*: lipase concentration in solution at equilibrium (U ml-1); Co: lipase concentration in the feed (U ml-1); d: resin particle diameter (cm); D: enzyme molecular diffusivity (cm2 min-1); E: axial dispersion coefficient (cm2 min-1); K: global mass transfer coefficient (cm2 min-1); kd: kinetic constant (U ml-1); k1: adsorption kinetic constant (ml U-1 min-1); k2: desorption kinetic constant (min-1); L: bed height (cm); Q: amount of adsorbed lipase in the resin (U ml-1 resin); Q*: amount of adsorbed lipase in the resin at equilibrium (U ml-1 resin); Qm: maximum adsorption capacity of the resin (U ml-1 resin); R: column radius (cm); Rs: rate of lipase adsorption (U ml-1 resin min-1); υ: superficial velocity (cm min-1); t: time; Z: column length (cm); ε: bed porosity. Subscript: i: time, j: position.2310781786Baillargeon, M.W., McCarthy, S.G., Geotrichum candidum NRRL Y-533 lipase: Purification, characterization and fatty acid specificity (1991) Lipids, 26, pp. 831-836Balcão, V.M., Vieira, M.C., Maleata, F.X., Adsorption of protein from several commercial lipase preparations onto hollow-fiber membrane module (1986) Biotechnol. Progr., 12, pp. 164-172Chase, H.A., Prediction of the performance of preparative affinity chromatography (1984) J. Chromatogr., 297, pp. 179-202Diogo, M.M., Silva, S., Cabral, J.M.S., Queiroz, J., A hydrophobic interaction on chromatography of Chromobacterium viscosum lipase on polypropylene glycol immobilised on Sepharose (1999) J. Chromatogr., 849, pp. 413-419Gitlesen, T., Bauer, M., Adlercreutz, P., Adsorption of lipase on polypropylene powder (1997) Biochim. Biophys. Acta, 1345, pp. 188-196Gustavsson, P.-E., Axelsson, A., Larsson, P.-O., Superporous agarose on bead as hydrophobic interaction chromatography support (1999) J. Chromatogr., 830, pp. 275-284Jacobsen, T., Olsen, J., Allerman, K., Substrate specifities of Geotrichum candidum lipase preparations (1990) Biotechnol. Lett., 12, pp. 121-126Kamimura, E.S., Mendieta, O., Sato, H.H., Pastore, G.M., Maugeri, F., Production of lipase from Geotrichum sp. and adsorption studies on affinity resin (1999) Brazilian J. Chem. Eng., 16, pp. 102-112Macêdo, G.A., Park, Y.K., Pastore, G.M., Partial purification and characterization of an extracellular lipase from a newly isolated strain of Geotrichum sp. (1997) Rev. Microbiol., 28, pp. 90-95Noriega, J., Tejada, A., Magaña, I., Ortega, J., Guzman, R., Modelling column regeneration effects on dye-ligand affinity chromatography (1997) Biotechnol. Prog., 13, pp. 296-300Phillips, A., Pretorious, H.J., Purification and characterization of an extracellular lipase of Galactomyces and Geotrichum (1991) Biotechnol. Lett., 13, pp. 833-838Porath, J., Salt promoted adsorption: Recent developments (1986) J. Chromatogr., 376, pp. 331-341Warwel, S., Borgdorf, R., Substrate selectivity of lipases in the esterification of cis-trans-isomers and positional isomers of conjugated linoleic acid (CLA) (2000) Biotechnol. Lett., 22, pp. 1151-115

Fungal Cutinases: Properties And Industrial Applications - Review [cutinases Fúngicas: Propriedades E Aplicações Industriais]

Cutinases (EC 3.1.1.74) are also known as cutin hidrolases. These enzymes share catalytic properties of lipases and esterases, presenting a unique feature of being active regardless the presence of an oil-water interface, making them interesting as biocatalysts in several industrial processes involving hydrolysis, esterification and trans-esterification reactions. They are also active in different reaction media, allowing their applications in different areas such as food industry, cosmetics, fine chemicals, pesticide and insecticide degradation, treatment and laundry of fiber textiles and polymer chemistry. The present review describes the characteristics, potential applications and new perspectives for these enzymes.31821182123Breccia, J.D., Krook, M., Ohlin, M., Hatti-Kaul, R., (2003) Enzyme Microb. Technol, 33, p. 244Kerry, N.L., Abbey, M., (1997) Atherosclerosis, 135, p. 93Pio, T.A., Macedo, G.A., (2007) Enzyme Microb. Technol, 41, p. 613Villeneuve, P., Muderwha, J.M., Graille, J., Hass, M.J., (2000) J. Mol. Catal. B: Enzym, 9, p. 113http://www.brenda.uni-koeln.de, acessada em Setembro 2007Ferreira, B.S., Calado, C.R.C., Keulen, F., Fonseca, L.P., Cabral, J.M.S., Fonseca, M.M.R., (2004) J. Biotechnol, 109, p. 159Egmond, M.R., De Vlieg, J., (2000) Biochimie, 82, p. 1015Soares, C.M., Teixeira, V.H., Baptista, A.M., (2003) Biophys. J, 84, p. 1628Borreguero, I., Carvalho, C.M.L., Cabral, J.M.S., Sinisterra, J.V., Alcántara, A.R., (2001) J. Mol. Catal. B: Enzym, 11, p. 613Melo, E.P., Baptista, R.P., Cabral, J.M.S., (2003) J. Mol. Catal. B: Enzym, 22, p. 299Petersen, S.B., Johnson, P.H., Fojan, P., Petersen, E.I., Petersen, M.T.N., Hansen, S., Ishak, R.J., Hough, E., (1998) J. Biotechnol, 66, p. 11Kolattukudy, P.E.E., (1984) Lipases, p. 471. , Borgstrom, B, Brockman, T, eds, Elsevier: AmsterdamCarvalho, C.M.L., Serralheiro, M.L.M., Cabral, J.M.S., Aires-Barros, M.R., (1997) Enzyme Microb. Technol, 22, p. 117Carvalho, C.M.L., Aires-Barros, M.R., Cabral, J.M.S., (1999) Biotechnol. Bioeng, 60, p. 17Carvalho, C.M.L., Aires-Barros, M.R., Cabral, J.M.S., (2000) J. Biotechnol, 81, p. 1Melo, E.P., Costa, S.M.B., Cabral, J.M.S., Fojan, P., Petersen, S.B., (2003) Chem. Phys. Lipids, 124, p. 37Ternström, T., Svendsen, A., Akke, M., Adlercreutz, P., (2005) Biochim. Biophys. Acta, 1748, p. 74Sebastião, M.J., Cabral, J.M.S., Aires-Barros, M.R., (1993) Biotechnol. Bioeng, 42, p. 326Gonçalves, A.M., Schacht, E., Matthjis, G., Aires-Barros, M.R., Cabral, J.M.S., Gil, M.H., (1999) Enzyme Microb. Technol, 24, p. 60Macedo, G.A., Pio, T.F., (2005) Braz. J. Microbiol, 36, p. 388Filipsen, J.A.C., Appel, A.C.M., Van Der Hidjen, H.T.W.M., Ve- Verrips, C.T., (1998) Enzyme Microb. Technol, 23, p. 274Klibanov, A.M., (2001) Nature, 409, p. 241Clauss, J., (1996) Óleos e Grãos, 5, p. 31Gonçalves, L.A.G., (1996) Óleos e Grãos, 5, p. 27Ahmed, J.I., (1995) Food Sci. Technol. Res, 9, p. 228Lima, J.R., Nassu, R.T., (1996) Quim. Nova, 19, p. 127Casey, J., Macrae, A.R., (1992) Inform, 3, p. 203Gunstone, F.D., (1999) Fett-Lipid, 101, p. 124Hammond, E. G.Glatz, B. A. Em Food BiotechnologyKling, R. D.Cheetham, P. S. J., eds.Elsevier Publishers Limited: Amsterdam, 1988Regado, M.A., Cristóvão, B.M., Moutinho, C.G., Balcão, V.M., Aires-Barros, R., Ferreira, J.P.M., Malcata, F.X., (2007) Int. J. Food Sci. Technol, 42, p. 961Gillies, B., Yamazaki, H., Armstrong, D.W., (1987) Biotechnol. Lett, 10, p. 709Armstrong, D. W.Gillies, B.Yamazaki, H. Em Flavour Chemistry, Trends and DevelopmentCharalambous, G., ed.Elsevier Science Publishers: New York, 1989Longo, M.A., Sanromán, M.A., (2006) Food Technol. Biotechnol, 44, p. 335Vandamme, E.J., Soetaert, W., (2002) J. Chem. Technol. Biotechnol, 77, p. 1323Larsson, K.M., Adlercreutz, P., Mattiasson, B., (1990) Biotechnol. Bioeng, 36, p. 135Barros, D.P.C., Fonseca, L.P., Cabral, J.M.S., (2007) J. Biotechnol, 7, p. 188Silva, F.A.M., Borges, F., Guimarães, C., Lima, J.L.F.C., Matos, C., Reis, S., (2000) J. Agric. Food Chem, 48, p. 2122Espinoza, M.C.F., Villeneuve, P., (2005) J. Agric. Food Chem, 53, p. 2779Hills, G., (2003) Eur. J. Lipid Sci. Technol, 105, p. 601Stamatis, H., Sereti, V., Kolisis, F.M., (1999) J. Am. Oil Chem. Soc, 12, p. 1505Chambers, W.H.E., (1992) Organophosphates, Chemistry, Fate, and Effects, , Chambers, J. E, Levi, P. E, eds, Academic Press: San DiegoBarlas, M.E., (1996) Environ. Contam. Toxicol, 57, p. 705Indeerjeet, K., Mathur, R.P., Tandon, S.N., Prem, D., (1997) Biomed. Chromatogr, 11, p. 352Galloway, T., Handy, R., (2003) Ecotoxicology, 12, p. 345Kim, Y.H., Ahn, J.Y., Moom, S.H., Lee, J., (2006) Chemosphere, 60, p. 1349Walz, I., Schwack, W., (2007) Eur. Food Res. Technol, 225, p. 593Walz, I., Schwack, W., (2008) Eur. Food Res. Technol, 22 (6), p. 1135Silva, C.M., Carneiro, F., O'Neill, A., Fonseca, L.P., Cabral, J.M.S., Guebitz, G., Cavaco-Paulo, A., (2005) J. Polym. Sci, 43, p. 2448Carvalho, C.M.L., Aires-Barros, M.R., Cabral, J.M.S., (1998) Electron. J. Biotechnol, 1, p. 28Vertommen, M.A.M.E., Nierstrasz, V.A., Van Der Veer, M., Warmoeskerken, M.M.C.G., (2005) J. Biotechnol, 120, p. 376Fischer-Colbrie, G., Heumann, S., Liebminger, S., Almansa, E., Cavaco-Paulo, A., Gubitz, G.M., (2004) Biocatal. Biotransform, 22, p. 341Alisch, M., Feuerhack, A., Blosfeld, A., Andreaus, J., Zimmermann, W., (2004) Biocatal. Biotransform, 22, p. 347Matamá, T., Silva, C., O'Neill, A., Casal, M., Soares, C., Gubitz, G.M., Cavaco-Paulo, A., (2004) 3rd International Conference on Textile Biotechnology, , abstract 5Yoon, M., Kellis, J., Poulouse, A.J., (2002) AATCC Rev, 2, p. 33Creveld, L.D., Meijberg, W., Berendsen, H.J.C., Pepermans, H.A.M., (2001) Biophys. Chem, 92, p. 67Degani, O., Gepstein, S., Dosoretz, C.G., (2002) Appl. Biochem. Biotechnol, 102, p. 277Hunsen, M., Azim, A., Mang, H., Wallner, S.R., Ronkvist, A., Xie, W., Gross, R., (2007) Macromolecules, 40, p. 148Mueller, R.J., (2006) Process Biochem, 41, p. 2124Doi, Y., (1990) Microbial polyesters, , VCH Publishers: New YorkBastioli, C.E., (1995) Degradable polymers, principles and applications, , Scott, G, Gilead, D, eds, Chapman & Hall: LondonMayer, J.M., Kaplin, D.L., (1994) Trends Polym. Sci, 2, p. 227Murphy, C.A., Cameron, J.A., Huang, S.J., Vinopal, R.T., (1996) Appl. Environ. Microbiol, 62, p. 456Sung, H.H., Kao, W.Y., Su, Y.J., (2003) Aquat. Toxicol, 66, p. 25Chang, B.V., Yang, C.M., Cheng, C.H., Yuan, S.Y., (2004) Chemosphere, 55, p. 533Kavlock, R., Boekelheide, K., Chapin, R., Cunningham, M., Faustman, E., Foster, P., Golub, M., Henderson, R., (2002) Reprod. Toxicol, 16, p. 709Carvalho, P.O., Calafatti, S.A., Marassi, M., Silva, D.M., Contesini, F.J., Bizaco, R., Macedo, G.A., (2005) Quim. Nova, 28, p. 614Mannesse, M.L.M., Cox, R.C., Koops, B.C., Verheij, H.M., Haas, G.H., Egmond, M., Van Der Hijden, H.T.W., Vlieg, J., (1995) Biochemistry, 34, p. 6400Andersen, A., Svendsen, A., Vind, J., Lassen, S.F., Hjort, C., Borch, K., Patkar, S.A., (2002) Colloids Surf. B: Biointerf, 26, p. 47http://v3.espacenet.com/textdoc?DB=EPODOC&IDX=WO8809367&F=0, acessada em Junho 2008http://v3.espacenet.com/textdoc?DB=EPODOC&IDX=JP3088897&F=0, acessada em Junho 2008http://v3.espacenet.com/textdoc?DB=EPODOC&IDX=NZ337239&F=0, acessada em Junho 2008http://v3.espacenet.com/textdoc?DB=EPODOC&IDX=EP1694903&F=0, acessada em Junho 2008http://v3.espacenet.com/textdoc?DB=EPODOC&IDX=JP2005058228&F=0, acessada em Junho 2008http://www.wikipatents.com/ca/2480912.html, acessada em Junho 2008http://www.wikipatents.com/ca/1262860.html, acessada em Junho 2008http://www.wikipatents.com/ca/2060510.html, acessada em Junho 2008http://www.wikipatents.com/ca/2465250.html, acessada em Junho 200

Supercritical Extraction Of Coumarin From Guaco (mikania Laevigata And Mikania Glomerata) For Pharmaceutical Applications

Guaco has several applications in the chemical and pharmaceutical industries. This plant is a source of coumarin, a diterpene compound with expectorants properties. In Brazil, guaco extracts are used as medicinal products authorized by the National Health Surveillance Agency (ANVISA). Studies proved that guaco extracts are also effective against cancer, ulcers and infectious diseases. Supercritical extractions have been widely used to obtain highly pure natural extracts that do not contain organic solvents and have not lost their thermosensitive compounds and therefore are suitable for pharmaceutical applications. In this study, coumarin was obtained from guaco leaves extracts by supercritical extraction under different experimental conditions according to a central composite rotational design (CCRD) with two independent variables, temperature (T) and pressure (P). Lower values of T were used to maintain the stability of thermosensitive compounds and because coumarin is highly soluble in supercritical CO2 even at low T. The responses surface analyzes (RSA) acquired by fitting of linear and quadratic models to experimental extraction yields and coumarin concentrations showed similar conditions for improving yields from both plant species. Although the extract yield values were similar for both plant species, coumarin concentrations were higher in Mikania laevigata than Mikania glomerata. The greater extract yield of 4.20% coumarin, related to the higher coumarin concentration (0.43 g × 100 g-1 in guaco leaves), was obtained under extraction conditions of P above 175 bar and T above 45 C. © 2013 Elsevier B.V. All rights reserved.836571Bolina, R.C., Garcia, E.F., Duarte, M.G.R., Estudo comparativo da composição química das espécies vegetais Mikania glomerata Sprengel e Mikania laevigata Schultz Bip. Ex Baker (2009) Revista Brasileira de Farmacognosia, 19, pp. 294-298Panizza, S., (1997) Plantas Que Curam, pp. 117-119. , Ibrasa São PauloOliveira, S., Akizue, G., (1995) Fundamentos de Farmacobotânica, , Atheneu São Paulo pp.149Rehder, V.L.G., Bighettia, A.E., Antonio, M.A., Kohn, L.K., Foglio, M.A., Possenti, A., Vilela, L., Carvalho, J.E., Antiulcerogenic activity of a crude hydroalcoholic extract and coumarin isolated from Mikania laevigata Schultz bip (2005) Phytomedicine, 12, pp. 72-77Celeghini, R.M.S., (1997) Extração e Análise Cromatográfica (HPLC) de Cumarinas em Plantas Medicinais Brasileiras, Tese de Doutorado, , Instituto de Química, Universidade de São PauloCeleghini, R.M.S., Vilegas, J.H.Y., Vilegas, F.M., Extraction and quantitative HPLC analysis of coumarin in hydroalcoholic extracts of Mikania glomerata Spreng, ("Guaco") leaves (2001) J. Brazilian Chemical Society, 12, pp. 706-709Cohen, A.J., Critical review of the toxicology of coumarin with special reference to interspecies differences in methabolism and hepatotoxic response and their significance to man (1979) Food and Chemical Toxicology, 17, pp. 277-289Zhou, Q., Wintersteen, C.L., Cadwallader, K.R., Identification and quantification of aroma-active components that contribute to the distinct malty flavor of buckwheat honey (2002) J. Agricultural and Food Chemistry, 50, pp. 2016-2021Opdyke, D.L.J., Monographs on fragrance raw materials: Coumarin (1974) Food and Cosmetics Toxicology, 12, pp. 385-388Fentem, J.H., Fry, J.R., Metabolism of coumarin by rat, gerbil and human livemicrosomes (1992) Xenobiotica, 22, pp. 357-367Michaeli, D., Molavi, A., Mirelman, D., Hanoch, A., Wein-Stein, L., Mode of action of coumermycin A: Comparisons with novobiocin (1970) Antimicrobians Agents Chemotherapy, 10, pp. 95-99Higgins, N.P., Peebles, C.L., Sugino, A., Cozzarelli, N.R., Purification of subunits of Escherichia coli DNA gyrase and reconstitution of enzymatic-activity (1978) Proceeding Academic Science, 75, pp. 1773-1777Paya, M., Halliwel, B., Hoult, J.R., Interactions of a Series of Coumarins with reactive oxygen species - Scavenging of superoxide, hypochlorous acid and hydroxyl radicals (1992) Biochemical Pharmacology, 44, pp. 205-214Leal, L.K.A.M., Ferreira, A.A.G., Bezerra, G.A., Matos, F.J.A., Viana, G.S.B., Antinociceptive, anti-inflammatory and bronchodilator activities of Brazilian medicinal plants containing coumarin: A comparative study (2000) J. Ethnopharmacology, 70, pp. 151-159Rostagno, M.A., Palma, M., Barroso, C.G., Ultrasound-assisted extraction of isoflavones from soy beverages blended with fruit juices (2007) Analytica Chimica Acta, 588, pp. 274-282Choksi, P.M., Joshi, V.Y., A review on lycopene - Extraction, purification, stability and applications (2007) International J. Food Properties, 10, pp. 289-298Wu, Z., Wu, S., Shi, X., Supercritical fluid extraction and determination of lutein in heterotrophically cultivated Chlorella pyrenoidosa (2007) J. Food Process Engineering, 30, pp. 174-185Marongiu, B., Piras, A., Porcedda, S., Tuveri, E., Maxia, A., Comparative analysis of the oil and supercritical CO2 extract of Ridolfia segetum (L.) Moris (2007) Natural Product Research, 21, pp. 412-417Coleman III, W.M., Dube, M.F., Ashraf-Khorassani, M., Taylor, L.T., Influence of plant growth stage on the essential oil content and composition in Davana (Artemisia pallens Wall.) (2007) J. Agricultural and Food Chemistry, 55, pp. 3037-3043Ling, J.Y., Zhang, G.Y., Cui, Z.J., Zhang, C.K., Supercritical fluid extraction of quinolizidine alkaloids from Sophora flavescens Ait. and purification by high-speed counter-current chromatography (2007) J. Chromatography A, 1145, pp. 123-127Bocevska, M., Sovová, H., Supercritical CO2 extraction of essential oil from yarrow (2007) J. Supercritical Fluids, 40, pp. 360-367Brunner, G., (1994) Gas Extraction An Introduction to Fundamentals of Supercritical Fluids and the Applications to Separation Processes, , Steinkopff Darmstadt Springer New York pp. 387Brunner, G., Supercritical fluids: Technology and application to food processing (2005) J. Food Engineering, 67, pp. 21-33Sandler, S.I., (1989) Chemical and Engineering Thermodynamics, , 2nd ed. Wiley Series USA pp. 622Rizvi, S.S.H., Benado, A.L., Zollweg, J.A., Daniels, J.A., Supercritical fluid extraction: Fundamental principles and modeling metods (1986) Food Technology, 40, pp. 55-65Abbas, K.A., Mohamed, A., Abdulamir, A.S., Abas, H.A., A review on supercritical fluid extraction as new analytical method (2008) American J. Biochemistry and Biotechnology, 4, pp. 345-353Gasparetto, J.C., Campos, F.R., Budel, J.M., Pontarolo, R., Mikania glomerata Spreng. E M. Laevigata Sch. Bip. Ex Baker, Asteraceae: Estudos agronômicos, genéticos, morfoanatômicos, químicos, farmacológicos, toxicológicos e uso nos programas de fitoterapia do Brasil (2010) Revista Brasileira de Farmacognosia, 20, pp. 627-640(1997) Official Methods of Analysis of AOAC International, , AOAC International ArligtonVilegas, J.H.Y., Marchi, E., Lanças, F.M., Extraction of low-polarity compounds (with emphasis on coumarin and kaurenoic acid) from Mikania glomerata ("guaco") leaves (1997) Phytochemical Analysis, 8, pp. 266-270Rodrigues, R.F., Tashima, A.K., Pereira, R.M.S., Mohamed, R.S., Cabral, F.A., Coumarin solubility and extraction from Emburana (Torresea cearensis) seeds with supercritical carbon dioxide (2008) J. Supercritical Fluids, 43, pp. 375-382Barros Neto, B., Scarmino, I.S., Bruns, R.E., (1996) Planejamento e Otimização Experimentos, , UNICAMP CampinasBox, G.E.P., Hunter, W.G., Hunter, J.S., (1978) Statistics for Experimenters - An Introduction of Design Data Analysis and Model Building, , Wiley New YorkRodrigues, R.F., (2005) Extração da Cumarina A Partir das Sementes da Emburana (Torresea Cearensis) Utilizando Dióxido de Carbono Supercrítico, pp. 33-36. , Dissertação de mestrado Unicamp CampinasVilegas, J.H.Y., Marchi, E., Lanças, F.M., Determination of coumarin and kaurenoic acid in Mikania glomerata ("guaco") leaves by high resolution gas chromatography (1997) Phytochemical Analysis, 8, pp. 74-77Mezzomo, N., Martínez, J., Ferreira, S.R.S., Supercritical fluid extraction of peach (Prunus persica) almond oil: Kinetics, mathematical modeling and scale-up (2009) J. Supercritical Fluids, 51, pp. 10-16Piantino, C.R., Supercritical CO2 extraction of phenolic compounds from Baccharis dracunculifolia (2008) J. Supercritical Fluids, 47, pp. 209-21