The SunBEAm Birth Cohort: Protocol Design

BACKGROUND: Food allergy (FA) and atopic dermatitis (AD) are common conditions that often present in the first year of life. Identification of underlying mechanisms and environmental determinants of FA and AD is essential to develop and implement effective prevention and treatment strategies. Objectives: We sought to describe the design of the Systems Biology of Early Atopy (SunBEAm) birth cohort. METHODS: Funded by the National Institute of Allergy and Infectious Diseases (NIAID) and administered through the Consortium for Food Allergy Research (CoFAR), SunBEAm is a US population-based, multicenter birth cohort that enrolls pregnant mothers, fathers, and their newborns and follows them to 3 years. Questionnaire and biosampling strategies were developed to apply a systems biology approach to identify environmental, immunologic, and multiomic determinants of AD, FA, and other allergic outcomes. RESULTS: Enrollment is currently underway. On the basis of an estimated FA prevalence of 6%, the enrollment goal is 2500 infants. AD is defined on the basis of questionnaire and assessment, and FA is defined by an algorithm combining history and testing. Although any FA will be recorded, we focus on the diagnosis of egg, milk, and peanut at 5 months, adding wheat, soy, cashew, hazelnut, walnut, codfish, shrimp, and sesame starting at 12 months. Sampling includes blood, hair, stool, dust, water, tape strips, skin swabs, nasal secretions, nasal swabs, saliva, urine, functional aspects of the skin, and maternal breast milk and vaginal swabs. CONCLUSIONS: The SunBEAm birth cohort will provide a rich repository of data and specimens to interrogate mechanisms and determinants of early allergic outcomes, with an emphasis on FA, AD, and systems biology

Efficacy of a Non-Hypercalcemic Vitamin-D2 Derived Anti-Cancer Agent (MT19c) and Inhibition of Fatty Acid Synthesis in an Ovarian Cancer Xenograft Model

BACKGROUND:Numerous vitamin-D analogs exhibited poor response rates, high systemic toxicities and hypercalcemia in human trials to treat cancer. We identified the first non-hypercalcemic anti-cancer vitamin D analog MT19c by altering the A-ring of ergocalciferol. This study describes the therapeutic efficacy and mechanism of action of MT19c in both in vitro and in vivo models. METHODOLOGY/PRINCIPAL FINDING:Antitumor efficacy of MT19c was evaluated in ovarian cancer cell (SKOV-3) xenografts in nude mice and a syngenic rat ovarian cancer model. Serum calcium levels of MT19c or calcitriol treated animals were measured. In-silico molecular docking simulation and a cell based VDR reporter assay revealed MT19c-VDR interaction. Genomewide mRNA analysis of MT19c treated tumors identified drug targets which were verified by immunoblotting and microscopy. Quantification of cellular malonyl CoA was carried out by HPLC-MS. A binding study with PPAR-Y receptor was performed. MT19c reduced ovarian cancer growth in xenograft and syngeneic animal models without causing hypercalcemia or acute toxicity. MT19c is a weak vitamin-D receptor (VDR) antagonist that disrupted the interaction between VDR and coactivator SRC2-3. Genome-wide mRNA analysis and western blot and microscopy of MT19c treated xenograft tumors showed inhibition of fatty acid synthase (FASN) activity. MT19c reduced cellular levels of malonyl CoA in SKOV-3 cells and inhibited EGFR/phosphoinositol-3kinase (PI-3K) activity independently of PPAR-gamma protein. SIGNIFICANCE:Antitumor effects of non-hypercalcemic agent MT19c provide a new approach to the design of vitamin-D based anticancer molecules and a rationale for developing MT19c as a therapeutic agent for malignant ovarian tumors by targeting oncogenic de novo lipogenesis

Fostering Collaborations and Partnerships Through OSEP Grants

Presenters from three universities discussed how Office of Special Education Programs (OSEP) grant projects have fostered interdisciplinary collaborations and school/community partnerships. This session discussed the powerful lessons that have emerged from those collaborations, which have involved related services personnel in schools (e.g., school psychology, school counseling, special education, speech and language pathology, and school social work), as well as community partners (e.g., transportation specialists, urban designers, and geographers)

Public Interest Technology

The impact of technology on our society is one of the defining challenges and opportunities of our time. In this context, it is critical to consider how technology and innovation affects not just our industrial systems, but entire political, social, and economic systems. Public interest technologies put people and society at the centre of our technological choices and strive to ensure that the benefits of technology are widely shared. However, there is limited understanding in Australia of the notion of public interest technology, in part because the use and meaning of the term is contingent on the ongoing and – in the wake of Covid – emerging questions about what constitutes the public interest. Drawing on perspectives from three experts in industry and academia, this forum socialises the concept of public interest technology, reveals recent applications of public interest technology, and issues a call to action for cross-sector engagement and collaboration to advance the development and implementation of public interest technology.</p

Webinar - Technology x Society Forum: Public Interest Technology

The impact of technology on our society is one of the defining challenges and opportunities of our time. In this context, it is critical to consider how technology and innovation affects not just our industrial systems, but entire political, social, and economic systems. Public interest technologies put people and society at the centre of our technological choices and strive to ensure that the benefits of technology are widely shared. However, there is limited understanding in Australia of the notion of public interest technology, in part because the use and meaning of the term is contingent on the ongoing and – in the wake of Covid – emerging questions about what constitutes the public interest. Drawing on perspectives from three experts in industry and academia, this forum socialises the concept of public interest technology, reveals recent applications of public interest technology, and issues a call to action for cross-sector engagement and collaboration to advance the development and implementation of public interest technology

Adapting to CONNECT: modifying a nursing home-based team-building intervention to improve hospital care team interactions, functioning, and implementation readiness

Background: Clinical interventions often need to be adapted from their original design when they are applied to new settings. There is a growing literature describing frameworks and approaches to deploying and documenting adaptations of evidence-based practices in healthcare. Still, intervention modifications are often limited in detail and justification, which may prevent rigorous evaluation of interventions and intervention adaptation effectiveness in new contexts. We describe our approach in a case study, combining two complementary intervention adaptation frameworks to modify CONNECT for Quality, a provider-facing team building and communication intervention designed to facilitate implementation of a new clinical program. Methods: This process of intervention adaptation involved the use of the Planned Adaptation Framework and the Framework for Reporting Adaptations and Modifications, for systematically identifying key drivers, core and non-core components of interventions for documenting planned and unplanned changes to intervention design. Results: The CONNECT intervention's original context and setting is first described and then compared with its new application. This lays the groundwork for the intentional modifications to intervention design, which are developed before intervention delivery to participating providers. The unpredictable nature of implementation in real-world practice required unplanned adaptations, which were also considered and documented. Attendance and participation rates were examined and qualitative assessment of reported participant experience supported the feasibility and acceptability of adaptations of the original CONNECT intervention in a new clinical context. Conclusion: This approach may serve as a useful guide for intervention implementation efforts applied in diverse clinical contexts and subsequent evaluations of intervention effectiveness

Ontogenetic variability in external morphology of native (Canadian) and non-native (Slovak) populations of pumpkinseed <i>Lepomis gibbosus</i> (Linnaeus 1758)

External morphology of native Canadian (River Otonabee, Looncall Lake) and non-native Slovak (River Danube) pumpkinseed was examined using both triple regression analysis (distance-based measurements) and geometrical analysis (coordinate-based measurements) within an ontogenetical aspect. In general, the results from the geometrical analysis comply with those from the triple regression analysis. The smallest pumpkinseed (predominantly juveniles) differed significantly from the largest pumpkinseed (predominantly adults) in all the three populations. The major difference was that adults had a deeper body and larger belly area than juveniles, which is probably associated with more space for gonads in mature fish. Developmental patterns and external morphology in pumpkinseed from the River Otonabee seemed to be closer to pumpkinseed from the Danube than to those from Lake Looncall. This suggests that, in the pumpkinseed examined, ontogenetic changes in external shape depend on environmental conditions (epigenetical information) rather than on geographical and/or genetical isolation. Further examination of early development, fecundity, number of spawning acts per season, parental care, egg size, age at maturation, etc., will follow to test this hypothesis