The Globular Cluster System of NGC 5128 II. Ages, Metallicities, Kinematics, and Formation

We present a study of the nearby post-merger giant elliptical galaxy, NGC 5128 (Centaurus A), in which we use the properties of its globular cluster (GC) and planetary nebula (PN) systems to constrain its evolution. Using photometric and spectroscopic data for 215 GCs presented in Paper I, we study trends in age, metallicity, and kinematics for the GC system. We confirm that the GC metallicity distribution is bimodal, and show that these two sub-populations have different properties. Using spectral line index measurements of the brightest clusters, the metal-poor GCs have old ages like the Milky Way globular clusters, while the metal-rich GCs have H-beta line-strengths that could be interpreted as a mean age of ~5 (+3/-2) Gyr. Both populations appear to have [Mg/Fe] ratios consistent with that of the Galactic GC system, although this quantity is not very well-constrained. The kinematics of the metal-rich GCs are similar to those of the planetary nebulae, exhibiting significant rotation about a misaligned axis, while the metal-poor GCs have a higher velocity dispersion and show a weaker kinematic correlation with the field stars. The total gravitating mass of NGC 5128 derived from the GCs is in excellent agreement with the value derived from stellar (PN) kinematics. We suggest that these and other data support a picture in which the main body of NGC 5128 was formed 3-8 Gyr ago by the dissipational merger of two unequal-mass disk galaxies supplemented by the continual accretion of both gas-rich and gas-poor satellites.Comment: 15 pages, 21 figures (figures 14-20 best viewed in color), accepted for publication in the Astrophysical Journa

Fashionably Late? Building up the Milky Way's Inner Halo

Using a sample of 248 metal-poor stars (RR Lyraes, red giants and RHB stars) which is remarkable for the accuracy of its 6-D kinematical data, we find a new component for the local halo which has an axial ratio c/a ~ 0.2, a similar flattening to the thick disk. It has a small prograde rotation but is supported by velocity anisotropy, and contains more intermediate-metallicity stars (with -1.5 < [Fe/H] < -1.0) than the rest of our sample. We suggest that this component was formed quite late, during or after the formation of the disk. It formed either from the gas that was accreted by the last major mergers experienced by the Galaxy, or by dynamical friction of massive infalling satellite(s) with the halo and possibly the stellar disk or thick disk. The remainder of the stars in our sample exhibit a clumpy distribution in energy and angular momentum, suggesting that the early, chaotic conditions under which the inner halo formed were not violent enough to erase the record of their origins. The clumpy structure suggests that a relatively small number of progenitors were responsible for building up the inner halo, in line with theoretical expectations. We find a difference in mean binding energy between the RR Lyrae variables and the red giants in our sample, suggesting that more of the RR Lyraes in the sample belong to the outer halo, and that the outer halo may be somewhat younger, as first suggested by Searle and Zinn (1978). We also find that the RR Lyrae mean rotation is more negative than the red giants, which is consistent with the recent result of Carollo et al.(2007) that the outer halo has a retrograde rotation and with the difference in kinematics seen between RR Lyraes and BHB stars by Kinman et al.(2007).Comment: 16 pages, 10 figures, this version accepted by Ap

Mapping the Galactic Halo I. The `Spaghetti' Survey

We describe a major survey of the Milky Way halo designed to test for kinematic substructure caused by destruction of accreted satellites. We use the Washington photometric system to identify halo stars efficiently for spectroscopic followup. Tracers include halo giants (detectable out to more than 100 kpc), blue horizontal branch stars, halo stars near the main sequence turnoff, and the ``blue metal-poor stars'' of Preston et al (1994). We demonstrate the success of our survey by showing spectra of stars we have identified in all these categories, including giants as distant as 75 kpc. We discuss the problem of identifying the most distant halo giants. In particular, extremely metal-poor halo K dwarfs are present in approximately equal numbers to the distant giants for V fainter than 18, and we show that our method will distinguish reliably between these two groups of metal-poor stars. We plan to survey 100 square degrees at high galactic latitude, and expect to increase the numbers of known halo giants, BHB stars and turnoff stars by more than an order of magnitude. In addition to the strong test that this large sample will provide for the question `was the Milky Way halo accreted from satellite galaxies?', we will improve the accuracy of mass measurements of the Milky Way beyond 50 kpc via the kinematics of the many distant giants and BHB stars we will find. We show that one of our first datasets constrains the halo density law over galactocentric radii of 5-20 kpc and z heights of 2-15 kpc. The data support a flattened power-law halo with b/a of 0.6 and exponent -3.0. More complex models with a varying axial ratio may be needed with a larger dataset.Comment: 55 pages, 22 figures, to appear in the Astronomical Journa

Exploring what patients with musculoskeletal conditions want from first point of contact health practitioners

Objectives This research was conducted to support the development of the Musculoskeletal (MSK) Health Capabilities Framework to ensure the framework reflected patients’ priorities. The aim of this study was to explore what patients with MSK problems want from their initial consultation with a first contact health practitioner and from the patient perspective what characterises a good first contact health practitioner. Methods Focus groups were held in four locations across England. Sixteen participants with a self-declared MSK condition aged 19 to 75 took part (11 female, five male). Participants discussed the questions they want answered when first going to see a health professional about a MSK problem and how they would describe a good first contact health provider. Results Participants wanted answers to questions about: the nature of the problem, the management of the problem, where to get information and support to help themselves, what activities they can do and what the future holds. Values and behaviours they expect and value from first contact health practitioners include: good communication skills, appreciation of impact, a willingness to discuss alternative and complementary therapies, shared decision making and an awareness of own limitations and when to refer. Conclusion The MSK core capabilities framework for first contact health practitioners aims to ensure a person-centred approach in the first stages of managing any MSK problem a person may present with. The focus groups enabled the developers of the framework to achieve a greater understanding of patient priorities, expectations and needs and allowed the patient perspective to be included in this national framework

Identification of Class I HLA T Cell Control Epitopes for West Nile Virus

The recent West Nile virus (WNV) outbreak in the United States underscores the importance of understanding human immune responses to this pathogen. Via the presentation of viral peptide ligands at the cell surface, class I HLA mediate the T cell recognition and killing of WNV infected cells. At this time, there are two key unknowns in regards to understanding protective T cell immunity: 1) the number of viral ligands presented by the HLA of infected cells, and 2) the distribution of T cell responses to these available HLA/viral complexes. Here, comparative mass spectroscopy was applied to determine the number of WNV peptides presented by the HLA-A*11:01 of infected cells after which T cell responses to these HLA/WNV complexes were assessed. Six viral peptides derived from capsid, NS3, NS4b, and NS5 were presented. When T cells from infected individuals were tested for reactivity to these six viral ligands, polyfunctional T cells were focused on the GTL9 WNV capsid peptide, ligands from NS3, NS4b, and NS5 were less immunogenic, and two ligands were largely inert, demonstrating that class I HLA reduce the WNV polyprotein to a handful of immune targets and that polyfunctional T cells recognize infections by zeroing in on particular HLA/WNV epitopes. Such dominant HLA/peptide epitopes are poised to drive the development of WNV vaccines that elicit protective T cells as well as providing key antigens for immunoassays that establish correlates of viral immunity. © 2013 Kaabinejadian et al

The sensitivity of primary productivity in Disko Bay, a coastal Arctic ecosystem, to changes in freshwater discharge and sea ice cover

The Greenland ice sheet is melting, and the rate of ice loss has increased 6-fold since the 1980s. At the same time, the Arctic sea ice extent is decreasing. Meltwater runoff and sea ice reduction both influence light and nutrient availability in the coastal ocean, with implications for the timing, distribution, and magnitude of phytoplankton production. However, the integrated effect of both glacial and sea ice melt is highly variable in time and space, making it challenging to quantify. In this study, we evaluate the relative importance of these processes for the primary productivity of Disko Bay, west Greenland, one of the most important areas for biodiversity and fisheries around Greenland. We use a high-resolution 3D coupled hydrodynamic–biogeochemical model for 2004–2018 validated against in situ observations and remote sensing products. The model-estimated net primary production (NPP) varied between 90–147 gC m−2 yr−1 during 2004–2018, a period with variable freshwater discharges and sea ice cover. NPP correlated negatively with sea ice cover and positively with freshwater discharge. Freshwater discharge had a strong local effect within ∼ 25 km of the source-sustaining productive hot spots during summer. When considering the annual NPP at bay scale, sea ice cover was the most important controlling factor. In scenarios with no sea ice in spring, the model predicted a ∼ 30 % increase in annual production compared to a situation with high sea ice cover. Our study indicates that decreasing ice cover and more freshwater discharge can work synergistically and will likely increase primary productivity of the coastal ocean around Greenland.publishedVersio

Modeled soil erosion potential is low across California's annual rangelands

We used the Revised Universal Soil Loss Equation (RUSLE) to evaluate how different residual forage dry matter (RDM) levels affect erosion potential in rangelands across California. The model was adapted to operate in a geographic information system (GIS) to model 14.8 million acres (6.0 million hectares) of land. Average erosion potential was low among all RDM scenarios and increased from an estimated 0.05 ton per acre per year (0.11 megagram per hectare per year) with the high RDM scenario to 0.12 ton per acre per year (0.27 megagram per hectare per year) with the low RDM scenario. Considering all RDM scenarios, fewer than 174,733 acres (70,710 hectares, or 1.2% of land) had erosion potential that exceeded soil loss tolerance values. Although achieving a uniform RDM target across a landscape may be an oversimplification of reality, simulations suggest that erosion potential on average is low in California's annual rangelands across high, moderate and low RDM recommendations. Moreover, our findings indicate that grazing management (maintaining moderate or high RDM) to mitigate erosion can be effective when targeted at areas of high vulnerability

Dose-response of myofibrillar protein synthesis to ingested whey protein during energy restriction in overweight postmenopausal women: a randomized, controlled trial

BackgroundDiet-induced weight loss is associated with a decline in lean body mass, as mediated by an impaired response of muscle protein synthesis (MPS). The dose-response of MPS to ingested protein, with or without resistance exercise, is well characterized during energy balance but limited data exist under conditions of energy restriction in clinical populations.ObjectiveTo determine the dose-response of MPS to ingested whey protein following short-term diet-induced energy restriction in overweight, postmenopausal, women at rest and postexercise.DesignForty middle-aged (58.6±0.4 y), overweight (BMI: 28.6±0.4), postmenopausal women were randomly assigned to 1 of 4 groups: Three groups underwent 5 d of energy restriction (∼800 kcal/d). On day 6, participants performed a unilateral leg resistance exercise bout before ingesting either a bolus of 15g (ERW15, n = 10), 35g (ERW35, n = 10) or 60g (ERW60, n = 10) of whey protein. The fourth group (n = 10) ingested a 35g whey protein bolus after 5 d of an energy balanced diet (EBW35, n = 10). Myofibrillar fractional synthetic rate (FSR) was calculated under basal, fed (FED) and postexercise (FED-EX) conditions by combining an L-[ring-13C6] phenylalanine tracer infusion with the collection of bilateral muscle biopsies.ResultsMyofibrillar FSR was greater in ERW35 (0.043±0.003%/h, P = 0.013) and ERW60 (0.042±0.003%/h, P = 0.026) than ERW15 (0.032 ± 0.003%/h), with no differences between ERW35 and ERW60 (P = 1.000). Myofibrillar FSR was greater in FED (0.044 ± 0.003%/h, P < 0.001) and FED-EX (0.048 ± 0.003%/h, P < 0.001) than BASAL (0.027 ± 0.003%/h), but no differences were detected between FED and FED-EX (P = 0.732) conditions. No differences in myofibrillar FSR were observed between EBW35 (0.042 ± 0.003%/h) and ERW35 (0.043 ± 0.003%/h, P = 0.744).ConclusionA 35 g dose of whey protein, ingested with or without resistance exercise, is sufficient to stimulate a maximal acute response of MPS following short-term energy restriction in overweight, postmenopausal women, and thus may provide a per serving protein recommendation to mitigate muscle loss during a weight loss program.Trial registryclinicaltrials.gov (ID: NCT03326284)

Surgical site infection after gastrointestinal surgery in high-income, middle-income, and low-income countries: a prospective, international, multicentre cohort study

Background: Surgical site infection (SSI) is one of the most common infections associated with health care, but its importance as a global health priority is not fully understood. We quantified the burden of SSI after gastrointestinal surgery in countries in all parts of the world. Methods: This international, prospective, multicentre cohort study included consecutive patients undergoing elective or emergency gastrointestinal resection within 2-week time periods at any health-care facility in any country. Countries with participating centres were stratified into high-income, middle-income, and low-income groups according to the UN's Human Development Index (HDI). Data variables from the GlobalSurg 1 study and other studies that have been found to affect the likelihood of SSI were entered into risk adjustment models. The primary outcome measure was the 30-day SSI incidence (defined by US Centers for Disease Control and Prevention criteria for superficial and deep incisional SSI). Relationships with explanatory variables were examined using Bayesian multilevel logistic regression models. This trial is registered with ClinicalTrials.gov, number NCT02662231. Findings: Between Jan 4, 2016, and July 31, 2016, 13 265 records were submitted for analysis. 12 539 patients from 343 hospitals in 66 countries were included. 7339 (58·5%) patient were from high-HDI countries (193 hospitals in 30 countries), 3918 (31·2%) patients were from middle-HDI countries (82 hospitals in 18 countries), and 1282 (10·2%) patients were from low-HDI countries (68 hospitals in 18 countries). In total, 1538 (12·3%) patients had SSI within 30 days of surgery. The incidence of SSI varied between countries with high (691 [9·4%] of 7339 patients), middle (549 [14·0%] of 3918 patients), and low (298 [23·2%] of 1282) HDI (p < 0·001). The highest SSI incidence in each HDI group was after dirty surgery (102 [17·8%] of 574 patients in high-HDI countries; 74 [31·4%] of 236 patients in middle-HDI countries; 72 [39·8%] of 181 patients in low-HDI countries). Following risk factor adjustment, patients in low-HDI countries were at greatest risk of SSI (adjusted odds ratio 1·60, 95% credible interval 1·05–2·37; p=0·030). 132 (21·6%) of 610 patients with an SSI and a microbiology culture result had an infection that was resistant to the prophylactic antibiotic used. Resistant infections were detected in 49 (16·6%) of 295 patients in high-HDI countries, in 37 (19·8%) of 187 patients in middle-HDI countries, and in 46 (35·9%) of 128 patients in low-HDI countries (p < 0·001). Interpretation: Countries with a low HDI carry a disproportionately greater burden of SSI than countries with a middle or high HDI and might have higher rates of antibiotic resistance. In view of WHO recommendations on SSI prevention that highlight the absence of high-quality interventional research, urgent, pragmatic, randomised trials based in LMICs are needed to assess measures aiming to reduce this preventable complication