Lithium production on a low-mass secondary in a black hole soft X-ray transient

We examine production of Li on the surface of a low-mass secondary in a black hole soft X-ray transient (BHSXT) through the spallation of CNO nuclei by neutrons which are ejected from a hot (> 10 MeV) advection-dominated accretion flow (ADAF) around the black hole. Using updated binary parameters, cross sections of neutron-induced spallation reactions, and mass accretion rates in ADAF derived from the spectrum fitting of multi-wavelength observations of quiescent BHSXTs, we obtain the equilibrium abundances of Li by equating the production rate of Li and the mass transfer rate through accretion to the black hole. The resulting abundances are found to be in good agreement with the observed values in seven BHSXTs. We note that the abundances vary in a timescale longer than a few months in our model. Moreover, the isotopic ratio Li6/Li7 is calculated to be about 0.7--0.8 on the secondaries, which is much higher than the ratio measured in meteorites. Detection of such a high value is favorable to the production of Li via spallation and the existence of a hot accretion flow, rather than an accretion disk corona system in quiescent BHSXT.Comment: 4 pages, 3 figures, and 2 tables, submitted to Astrophyscal Jounal Letter

P-Process Nucleosynthesis inside Supernova-Driven Supercritical Accretion Disks

We investigate p-process nucleosynthesis in a supercritical accretion disk around a compact object of 1.4 M_solar, using the self-similar solution of an optically thick advection dominated flow. Supercritical accretion is expected to occur in a supernova with fallback material accreting onto a new-born compact object. It is found that appreciable amounts of p-nuclei are synthesized via the p-process in supernova-driven supercritical accretion disks (SSADs) when the accretion rate m_dot = M_dot c^2/(16 L_Edd) >10^5, where L_Edd is the Eddington luminosity. Abundance profiles of p-nuclei ejected from SSADs have similar feature to those of the oxygen/neon layers in Type II supernovae when the abundance of the fallback gas far from the compact object is that of the oxygen/neon layers in the progenitor. The overall abundance profile is in agreement with that of the solar system. Some p-nuclei, such as Mo, Ru, Sn, and La, are underproduced in the SSADs as in Type II supernovae. If the fallback gas is mixed with a small fraction of proton through Rayleigh-Taylor instability during the explosion, significant amounts of Mo92 are produced inside the SSADs. Ru96 and La138 are also produced when the fallback gas contains abundant proton though the overall abundance profile of p-nuclei is rather different from that of the solar system. The p-process nucleosynthesis in SSADs contributes to chemical evolution of p-nuclei, in particular Mo92, if several percents of fallback matter are ejected via jets and/or winds.Comment: 15 pages, 7 figures included, 3 tables, LaTeX emulateapj5.sty, accepted for publication by the Astronomical Journal (March, 2003

Energy Extraction from a Rotating Black Hole by Magnetic Reconnection in Ergosphere

We investigate mechanisms of energy extraction from a rotating black hole in terms of negative energy-at-infinity. In addition to the Penrose process through particle fission, the Blandford-Znajek mechanism by magnetic tension, and the magnetohydrodynamic Penrose process, we examine energy extraction from a black hole caused by magnetic reconnection in the ergosphere. The reconnection redistributes the angular momentum efficiently to yield the negative energy-atinfinity. We derive a condition for the process to operate in a simple situation, where the plasma is incompressible and the magnetic energy is converted completely to the plasma kinetic energy locally. Astrophysical situations of magnetic reconnection around the black holes are also discussed.Comment: 26 pages, 8 figures, to be published in The Astrophysical Journa

Requirement of Retinoic Acid Receptor β for Genipin Derivative-Induced Optic Nerve Regeneration in Adult Rat Retina

Like other CNS neurons, mature retinal ganglion cells (RGCs) are unable to regenerate their axons after nerve injury due to a diminished intrinsic regenerative capacity. One of the reasons why they lose the capacity for axon regeneration seems to be associated with a dramatic shift in RGCs\u27 program of gene expression by epigenetic modulation. We recently reported that (1R)-isoPropyloxygenipin (IPRG001), a genipin derivative, has both neuroprotective and neurite outgrowth activities in murine RGC-5 retinal precursor cells. These effects were both mediated by nitric oxide (NO)/S-nitrosylation signaling. Neuritogenic activity was mediated by S-nitrosylation of histone deacetylase-2 (HDAC2), which subsequently induced retinoic acid receptor β (RARβ) expression via chromatin remodeling in vitro. RARβ plays important roles of neural growth and differentiation in development. However, the role of RARβ expression during adult rat optic nerve regeneration is not clear. In the present study, we extended this hypothesis to examine optic nerve regeneration by IPRG001 in adult rat RGCs in vivo. We found a correlation between RARβ expression and neurite outgrowth with age in the developing rat retina. Moreover, we found that IPRG001 significantly induced RARβ expression in adult rat RGCs through the S-nitrosylation of HDAC2 processing mechanism. Concomitant with RARβ expression, adult rat RGCs displayed a regenerative capacity for optic axons in vivo by IPRG001 treatment. These neuritogenic effects of IPRG001 were specifically suppressed by siRNA for RARβ. Thus, the dual neuroprotective and neuritogenic actions of genipin via S-nitrosylation might offer a powerful therapeutic tool for the treatment of RGC degenerative disorders. © 2013 Koriyama et al

Neuritogenic activity of a genipin derivative in retinal ganglion cells is mediated by retinoic acid receptor β expression through nitric oxide/S-nitrosylation signaling

Genipin, a herbal iridoid, is known to have both neuroprotective and neuritogenic activity in neuronal cell lines. As it is structurally similar to tetrahydrobiopterin, its activity is believed to be nitric oxide (NO)-dependent. We previously proposed a novel neuroprotective activity of a genipin derivative, (1R)-isoPropyloxygenipin (IPRG001), whereby it reduces oxidative stress in RGC-5, a neuronal precursor cell line of retinal origin through protein S-nitrosylation. In the present study, we investigated another neuritogenic property of IPRG001 in RGC-5 cells and retinal explant culture where in we focused on the NO-cGMP-dependent and protein S-nitrosylation pathways. IPRG001 stimulated neurite outgrowth in RGC-5 cells and retinal explant culture through NO-dependent signaling, but not NO-dependent cGMP signaling. Neurite outgrowth with IPRG001 requires retinoic acid receptor β (RARβ) expression, which is suppressed by an RAR blocking agent and siRNA inhibition. Thereby, we hypothesized that RARβ expression is mediated by protein S-nitrosylation. S-nitrosylation of histone deacetylase 2 is a key mechanism in chromatin remodeling leading to transcriptional gene activation. We found a parallelism between S-nitrosylation of histone diacetylase 2 and the induction of RARβ expression with IPRG001 treatment. The both neuroprotective and neuritogenic activities of genipin could be a new target for the regeneration of retinal ganglion cells after glaucomatous conditions. © 2011 International Society for Neurochemistry

In vitro modeling to determine mutation specificity of EGFR tyrosine kinase inhibitors against clinically relevant EGFR mutants in non-small-cell lung cancer

EGFR mutated lung cancer accounts for a significant subgroup of non-small-cell lung cancer (NSCLC). Over the last decade, multiple EGFR tyrosine kinase inhibitors (EGFR-TKIs) have been developed to target mutated EGFR. However, there is little information regarding mutation specific potency of EGFR-TKIs against various types of EGFR mutations. The purpose of this study is to establish an in vitro model to determine the “therapeutic window” of EGFR-TKIs against various types of EGFR mutations, including EGFR exon 20 insertion mutations. The potency of 1st (erlotinib), 2nd (afatinib) and 3rd (osimertinib and rociletinib) generation EGFR-TKIs was compared in vitro for human lung cancer cell lines and Ba/F3 cells, which exogenously express mutated or wild type EGFR. An in vitro model of mutation specificity was created by calculating the ratio of IC50 values between mutated and wild type EGFR. The in vitro model identified a wide therapeutic window of afatinib for exon 19 deletions and L858R and of osimertinib and rociletinib for T790M positive mutations. The results obtained with our models matched well with previously reported preclinical and clinical data. Interestingly, for EGFR exon 20 insertion mutations, most of which are known to be resistant to 1st and 2nd generation EGFR-TKIS, osimertinib was potent and presented a wide therapeutic window. To our knowledge, this is the first report that has identified the therapeutic window of osimertinib for EGFR exon 20 insertion mutations. In conclusion, this model will provide a preclinical rationale for proper selection of EGFR-TKIs against clinically-relevant EGFR mutations

Effect of shelter acclimation on the post-release movement and putative predation mortality of hatchery-reared black-spot tuskfish Choerodon schoenleinii, determined by acoustic telemetry

In this study, the effect of shelter acclimation on the post-release movement and putative predation mortality of hatchery-reared black-spot tuskfish Choerodon schoenleinii was examined using acoustic telemetry. We acclimated four 1-year-old fish to shelters in cages before release and compared their movements with six nonacclimated fish. Since it was not possible to compare the behavioral pattern between the former and the latter fish due to the short periods the latter fish were available to be monitored, we also compared their movements with those of large nonacclimated fish that were less likely to be preyed upon. Sixty-seven percent of the nonacclimated fish showed atypical movements before the signals ceased to be detected, a pattern that suggested a predation event had occurred, whereas none of the acclimated and large nonacclimated fish showed the atypical movements. In addition, the probability of detection cessation was about 13 times lower in the acclimated than nonacclimated fish. The signal detection patterns suggest that the acclimated fish utilized night-time shelters from the first night after release, while the large nonacclimated fish started to utilize shelters several days after release. Therefore, it is likely that the shelter acclimation enhanced the shelter utilization by tuskfish, possibly decreasing post-release predation mortality

A new technique for monitoring grazing behavior of Hawksbill turtles (Eretmochelys imbricata) using acceleration data loggers

Organized by Graduate School of Informatics, Kyoto University ; JSPS Bangkok Liaison Office ; Japanese Society of Bio-logging Science ; Informatics Research Center for Development of Knowledge Society InfrastructureDecember 13-15, 2004, Imperial Tara Hotel, Bangkok, ThailandGrazing behavior of sea turtles is important to understand their behavioral ecology. However, there is a shortage of effective techniques available for monitoring the grazing behavior accurately over a long period. In this study, the grazing behavior of hawksbill turtles (Eretmochelys imbricata) was monitored with acceleration data loggers which recorded depth, temperature, and accelerations in two axes. A Juvenile hawksbill turtle was attached with two acceleration data loggers on both head and carapace. During the experiment, we recorded the behavior of turtles on the underwater digital video camera. Their behaviors were distinguished into four patterns through the acceleration profiles and the underwater observation as follows; resting, swimming, grazing and breathing. The new technique can clarify when and where turtles graze quantitatively as well as time allocation of their behavior patterns