Application of 3D printing to prototype and develop novel plant tissue culture systems

Additional file 6. STL (StereoLithography) file was designed for an accessory lid without fan slot using SketchUp or Fusion 360 (Autodesk) software and the STL file was processed using MatterControl 3D printing software and exported as gcode files

3D printing for the development and evaluation of three novel liquid media systems for plant tissue culture

Labour represents a significant portion of the cost for micropropagated plants, and automation is a potential solution to increase efficiency. Micropropagation most often uses semisolid media, but many authors suggest that liquid systems are the key to developing efficient automated systems. However, liquid cultures require ancillary pneumatic or mechanical systems to prevent hypoxia or hyperhydricity of tissues. In this study, three novel liquid culture systems were designed, produced, and evaluated. The design and production of these systems was enabled using low-cost consumer-grade 3D printing technology. The first system uses hydrostatics to enable passive thin-media layer liquid culture. The second uses a two-pieces scaffold to facilitate rooting in liquid media. The third device overcomes the main disadvantages of mechanical temporary immersion systems by allowing full immersion timing control and a small footprint. Together, these novel approaches to liquid culture offer new choices to industry and may help move toward increased automation

Biofunctionalized Zinc Oxide Field Effect Transistors for Selective Sensing of Riboflavin with Current Modulation

Zinc oxide field effect transistors (ZnO-FET), covalently functionalized with single stranded DNA aptamers, provide a highly selective platform for label-free small molecule sensing. The nanostructured surface morphology of ZnO provides high sensitivity and room temperature deposition allows for a wide array of substrate types. Herein we demonstrate the selective detection of riboflavin down to the pM level in aqueous solution using the negative electrical current response of the ZnO-FET by covalently attaching a riboflavin binding aptamer to the surface. The response of the biofunctionalized ZnO-FET was tuned by attaching a redox tag (ferrocene) to the 3′ terminus of the aptamer, resulting in positive current modulation upon exposure to riboflavin down to pM levels

Regeneration of shoots from immature and mature inflorescences of Cannabis sativa

Cannabis sativais usually clonally propagated from plants in the vegetative phase. However, phenotypic traits such as yield and chemical composition can only be assessed in unfertilized plants reaching the end of their life cycle and there are no peer-reviewed methods to propagate flowering plants. In this study, immature (three cultivars) and mature (one cultivar) floral explants were cultured on thidiazuron and shoot development was observed in both the immature and mature explants. This provides the first report of micropropagation from floral tissues in C. sativaand will enable plants to be clonally propagated up to the date of harvestThe accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author

Regeneration of shoots from immature and mature inflorescences of<i>Cannabis sativa</i>

Cannabis sativa is usually clonally propagated from plants in the vegetative phase. However, phenotypic traits such as yield and chemical composition can only be assessed in unfertilized plants reaching the end of their life cycle and there are no peer-reviewed methods to propagate flowering plants. In this study, immature (three cultivars) and mature (one cultivar) floral explants were cultured on thidiazuron and shoot development was observed in both the immature and mature explants. This provides the first report of micropropagation from floral tissues in C. sativa and will enable plants to be clonally propagated up to the date of harvest.</jats:p

MOESM1 of Application of 3D printing to prototype and develop novel plant tissue culture systems

Additional file 1: Figure S1. Variation in spectra of light across the shelf area represented by contour plot / heat map of Correlated Colour Temperature (CCT) values over a shelf