Immunohistochemical detection of laminin-1 and Ki-67 in radicular cysts and keratocystic odontogenic tumors

<p>Abstract</p> <p>Background</p> <p>Odontogenic cysts are those which arise from the epithelium associated with the development of teeth. Some odontogenic cysts were found to have special biological features that make them distinct from other lesions. This study was conducted to detect the immunoepxression of laminin-1 and Ki-67 in both radicular cysts (RCs) and keratocystic odontogenic tumors (KCOTs) and to examine the possible predictive value of these markers.</p> <p>Methods</p> <p>Thirteen cases of RCs and twelve cases of KCOTs were included in this study. Antibodies against laminin-1 and Ki-67 were used as primary antibodies.</p> <p>Results</p> <p>ten cases out of thirteen cases of RCs were immunopositive to laminin-1. The immunonegative cases of RCs showed high degree of inflammation inside the connective tissue wall. One case out of twelve cases of KCOTs was immunopositive to laminin-1 and the rest were immunonegative. Seven cases out of thirteen cases of RCs showed immunopositivity for Ki-67 with increased numbers of immunopositive cells when the inflammation was severe in the connective tissue wall. All KCOTS were immunopositive to Ki-67.</p> <p>Conclusions</p> <p>The benign nature of radicular cysts and the aggressive behavior of keratocystic odontogenic tumors could be explained by the expression of laminin and Ki-67. Laminin-1 and Ki-67 could be valuable markers for the prediction of the biologic behavior of cystic lesions.</p

Conhecimento e práticas de autocuidado na prevenção do pé diabético

DOI: https://doi.org/10.31948/rev.criterios/26.1-art3 Objective: To evaluate the knowledge and practices of self-care to prevent diabetic foot in people with Type 2 Diabetes Mellitus. Methodology: observational, descriptive, cross-sectional study, through which information was obtained by survey about knowledge and practices to prevent diabetic foot, in patients with diabetes mellitus type 2, without diagnosis of the complication. Sample of 70 patients. Results: 68.6% of the participants have a high level of knowledge regarding their disease and the prevention of diabetic foot; the minority of people is located at a level of knowledge equivalent to 4.3%. Regarding the practices for the prevention of diabetic foot, it was evidenced that 63.2% perform adequate self-care practices, although there is a lack of adherence to pharmacological treatment. Conclusions: There is a high level of knowledge, adequate practices in the prevention of diabetic foot and lack of therapeutic adherence. It is necessary to continue creating strategies that allow greater knowledge and motivation in the exercise of appropriate practices including adherence.Objetivo: Evaluar los conocimientos y prácticas de autocuidado para prevenir pie diabético en personas con Diabetes Mellitus tipo 2. Metodología: estudio observacional, descriptivo, transversal, mediante el cual se obtuvo información por encuesta acerca de conocimientos y prácticas para prevenir pie diabético, en una muestra de 70 pacientes con diabetes mellitus tipo 2 y sin diagnóstico de la complicación. Resultados: El 68,6 % de los participantes tiene un alto nivel de conocimientos con respecto a su enfermedad y la prevención del pie diabético; la minoría de las personas se ubica en un nivel de conocimiento bajo, equivalente al 4,3 %. Con relación a las prácticas para prevención del pie diabético, se evidenció que el 63,2 % realiza adecuadas prácticas de autocuidado; no obstante, existe falta de adherencia al tratamiento farmacológico. Conclusiones: Existe alto nivel de conocimiento, prácticas adecuadas en la prevención del pie diabético y falta de adherencia terapéutica; se hace necesario continuar creando estrategias que permitan mayor conocimiento y motivación en el ejercicio de las prácticas adecuadas, incluida la adherencia.DOI: https://doi.org/10.31948/rev.criterios/26.1-art3 Objetivo: Avaliar as práticas de conhecimento e auto-cuidado para evitar pé diabético em pessoas com diabetes mellitus tipo 2. Metodologia: Estudo observacional, descritivo, transversal, pelo qual a informação foi obtida por pesquisa de conhecimentos e práticas para prevenir pacientes de pé diabético com diabetes mellitus tipo 2, sem diagnóstico da complicação, com uma amostra de 70 pacientes. Resultados: 68,6% dos participantes possuem um alto nível de conhecimento sobre a doença e a prevenção do pé diabético; a minoria de pessoas está localizada em um nível de conhecimento equivalente a 4,3%. Em relação às práticas de prevenção do pé diabético, evidenciou-se que 63,2% realizaram práticas adequadas de autocuidado, embora haja falta de adesão ao tratamento farmacológico. Conclusões: Existe um alto nível de conhecimento, práticas adequadas na prevenção do pé diabético e falta de adesão terapêutica. É necessário continuar a criar estratégias que permitam maior conhecimento e motivação no exercício de práticas adequadas, incluindo a adesão

Chromosomics: Bridging the Gap between Genomes and Chromosomes

The recent advances in DNA sequencing technology are enabling a rapid increase in the number of genomes being sequenced. However, many fundamental questions in genome biology remain unanswered, because sequence data alone is unable to provide insight into how the genome is organised into chromosomes, the position and interaction of those chromosomes in the cell, and how chromosomes and their interactions with each other change in response to environmental stimuli or over time. The intimate relationship between DNA sequence and chromosome structure and function highlights the need to integrate genomic and cytogenetic data to more comprehensively understand the role genome architecture plays in genome plasticity. We propose adoption of the term ‘chromosomics’ as an approach encompassing genome sequencing, cytogenetics and cell biology, and present examples of where chromosomics has already led to novel discoveries, such as the sex-determining gene in eutherian mammals. More importantly, we look to the future and the questions that could be answered as we enter into the chromosomics revolution, such as the role of chromosome rearrangements in speciation and the role more rapidly evolving regions of the genome, like centromeres, play in genome plasticity. However, for chromosomics to reach its full potential, we need to address several challenges, particularly the training of a new generation of cytogeneticists, and the commitment to a closer union among the research areas of genomics, cytogenetics, cell biology and bioinformatics. Overcoming these challenges will lead to ground-breaking discoveries in understanding genome evolution and functio

Conocimientos y prácticas de autocuidado en la prevención del pie diabético

Objetivo: Evaluar los conocimientos y prácticas de autocuidado para prevenir pie diabético en personas con Diabetes Mellitus tipo 2. Metodología: estudio observacional, descriptivo, transversal, mediante el cual se obtuvo información por encuesta acerca de conocimientos y prácticas para prevenir pie diabético, en una muestra de 70 pacientes con diabetes mellitus tipo 2 y sin diagnóstico de la complicación. Resultados: El 68,6 % de los participantes tiene un alto nivel de conocimientos con respecto a su enfermedad y la prevención del pie diabético; la minoría de las personas se ubica en un nivel de conocimiento bajo, equivalente al 4,3 %. Con relación a las prácticas para prevención del pie diabético, se evidenció que el 63,2 % realiza adecuadas prácticas de autocuidado; no obstante, existe falta de adherencia al tratamiento farmacológico. Conclusiones: Existe alto nivel de conocimiento, prácticas adecuadas en la prevención del pie diabético y falta de adherencia terapéutica; se hace necesario continuar creando estrategias que permitan mayor conocimiento y motivación en el ejercicio de las prácticas adecuadas, incluida la adherencia.</jats:p

Chromosomics: Bridging the Gap between Genomes and Chromosomes

The recent advances in DNA sequencing technology are enabling a rapid increase in the number of genomes being sequenced. However, many fundamental questions in genome biology remain unanswered, because sequence data alone is unable to provide insight into how the genome is organised into chromosomes, the position and interaction of those chromosomes in the cell, and how chromosomes and their interactions with each other change in response to environmental stimuli or over time. The intimate relationship between DNA sequence and chromosome structure and function highlights the need to integrate genomic and cytogenetic data to more comprehensively understand the role genome architecture plays in genome plasticity. We propose adoption of the term ‘chromosomics’ as an approach encompassing genome sequencing, cytogenetics and cell biology, and present examples of where chromosomics has already led to novel discoveries, such as the sex-determining gene in eutherian mammals. More importantly, we look to the future and the questions that could be answered as we enter into the chromosomics revolution, such as the role of chromosome rearrangements in speciation and the role more rapidly evolving regions of the genome, like centromeres, play in genome plasticity. However, for chromosomics to reach its full potential, we need to address several challenges, particularly the training of a new generation of cytogeneticists, and the commitment to a closer union among the research areas of genomics, cytogenetics, cell biology and bioinformatics. Overcoming these challenges will lead to ground-breaking discoveries in understanding genome evolution and function.</jats:p

Chromosomics: Bridging the Gap between Genomes and Chromosomes

The recent advances in DNA sequencing technology are enabling a rapid increase in the number of genomes being sequenced. However, many fundamental questions in genome biology remain unanswered, because sequence data alone is unable to provide insight into how the genome is organised into chromosomes, the position and interaction of those chromosomes in the cell, and how chromosomes and their interactions with each other change in response to environmental stimuli or over time. The intimate relationship between DNA sequence and chromosome structure and function highlights the need to integrate genomic and cytogenetic data to more comprehensively understand the role genome architecture plays in genome plasticity. We propose adoption of the term 'chromosomics' as an approach encompassing genome sequencing, cytogenetics and cell biology, and present examples of where chromosomics has already led to novel discoveries, such as the sex-determining gene in eutherian mammals. More importantly, we look to the future and the questions that could be answered as we enter into the chromosomics revolution, such as the role of chromosome rearrangements in speciation and the role more rapidly evolving regions of the genome, like centromeres, play in genome plasticity. However, for chromosomics to reach its full potential, we need to address several challenges, particularly the training of a new generation of cytogeneticists, and the commitment to a closer union among the research areas of genomics, cytogenetics, cell biology and bioinformatics. Overcoming these challenges will lead to ground-breaking discoveries in understanding genome evolution and function

Chromosomics : bridging the gap between genomes and chromosomes

The recent advances in DNA sequencing technology are enabling a rapid increase in the number of genomes being sequenced. However, many fundamental questions in genome biology remain unanswered, because sequence data alone is unable to provide insight into how the genome is organised into chromosomes, the position and interaction of those chromosomes in the cell, and how chromosomes and their interactions with each other change in response to environmental stimuli or over time. The intimate relationship between DNA sequence and chromosome structure and function highlights the need to integrate genomic and cytogenetic data to more comprehensively understand the role genome architecture plays in genome plasticity. We propose adoption of the term 'chromosomics' as an approach encompassing genome sequencing, cytogenetics and cell biology, and present examples of where chromosomics has already led to novel discoveries, such as the sex-determining gene in eutherian mammals. More importantly, we look to the future and the questions that could be answered as we enter into the chromosomics revolution, such as the role of chromosome rearrangements in speciation and the role more rapidly evolving regions of the genome, like centromeres, play in genome plasticity. However, for chromosomics to reach its full potential, we need to address several challenges, particularly the training of a new generation of cytogeneticists, and the commitment to a closer union among the research areas of genomics, cytogenetics, cell biology and bioinformatics. Overcoming these challenges will lead to ground-breaking discoveries in understanding genome evolution and function