Fibrin association at hybrid biointerfaces made of clot-binding peptides and polythiophene

The properties as biointerfaces of electroactive conducting polymer-peptide biocomposites formed by poly(3,4-ethylenedioxythiophene) (PEDOT) and CREKA or CR(NMe)EKA peptide sequences (where Glu has been replaced by N-methyl-Glu in the latter) have been compared. CREKA is a linear pentapeptide that recognizes clotted plasma proteins and selectively homes to tumors, while CR(NMe)EKA is an engineer to improve such properties by altering peptide-fibrin interactions. Differences between PEDOT-CREKA and PEDOT-CR(NMe)EKA reflect dissemblance in the organization of the peptides into the polymeric matrix. Both peptides affect fibrinogen thrombin-catalyzed polymerization causing the immediate formation of fibrin, whereas in the absence of thrombin this phenomenon is only observed for CR(NMe)EKA. Consistently, the fibrin-adsorption capacity is higher for PEDOT-CR(NMe)EKA than for PEDOT-CREKA, even though in both cases adsorbed fibrin exhibits round-like morphologies rather than the characteristic fibrous structure. PEDOT-peptide films coated with fibrin are selective in terms of cell adhesion, promoting the attachment of metastatic cells with respect to normal cells.Peer ReviewedPostprint (author's final draft

Conformal Robotic Stereolithography

Additive manufacturing by layerwise photopolymerization, commonly called stereolithography (SLA), is attractive due to its high resolution and diversity of materials chemistry. However, traditional SLA methods are restricted to planar substrates and planar layers that are perpendicular to a single-axis build direction. Here, we present a robotic system that is capable of maskless layerwise photopolymerization on curved surfaces, enabling production of large-area conformal patterns and the construction of conformal freeform objects. The system comprises an industrial six-axis robot and a custom-built maskless projector end effector. Use of the system involves creating a mesh representation of the freeform substrate, generation of a triangulated toolpath with curved layers that represents the target object to be printed, precision mounting of the substrate in the robot workspace, and robotic photopatterning of the target object by coordinated motion of the robot and substrate. We demonstrate printing of conformal photopatterns on spheres of various sizes, and construction of miniature three-dimensional objects on spheres without requiring support features. Improvement of the motion accuracy and development of freeform toolpaths would enable construction of polymer objects that surpass the size and support structure constraints imparted by traditional SLA systems.American Society for Engineering Education. National Defense Science and Engineering Graduate FellowshipNational Institute of Mental Health (U.S.) (University of Michigan Microfluidics in Biomedical Sciences Training Program. 5T32-EB005582)Singapore-MIT Alliance for Research and Technology (SMART

Conversations as a Holistic Assessment Tool : An Action Research Report

As Japanese educational policy places greater emphasis on spoken communication skills (MEXT, 26 September 2014), a greater need arises for appropriate assessments that can provide meaningful information to all stakeholders. This paper presents data collected from student performances on an experimental spoken English assessment task conducted at a Japanese university. Designed as the foundation for a reconstructed English communication curriculum for first-year university students enrolled in compulsory English classes (Tempest, 2018), the assessment task elicited free-form conversation, which was then transcribed and analyzed for specific metrics of spoken L2 output. Data deriving from student-generated transcriptions of recorded conversations included total words spoken, total turns taken, average turn length, and longest turn length. Preliminary analysis of the data indicates positive student gains over the course of the semester. As an investigative report of the assessment task trial period, few claims can be made at this point. However, future studies are forthcoming with support from JSPS KAKENHI grant number 19K13309

Report on New PBL Elective Course: Adventure Education and Event Planning

This report outlines the development, implementation, and evaluation of an eight-week experimental project-based learning (PBL) elective course that was trialed during the spring semester of 2023 at Sojo University. The course project consisted of a real-world educational and recreational event attended by local elementary and junior high school students. The report serves as a process-oriented example for potential course authors of similar elective courses featuring experiential and project-based learning, as well as an outcomes-based justification for future development of a related community program. 本報告書は、崇城⼤学で 2023 年度前期（春学期）に試⾏された、8 週間の実験的プロジ ェクトベース学習（PBL）選択コースの開発、実施、評価を概説したものである。このコ ースのプロジェクトは、地元の⼩中学⽣が参加する現実的教育及びレクリエーション活動 で構成された。 本報告書は、同様の体験学習型選択コースの作成者に向けプロセス指向 の例として、また将来のコミュニティ・プログラム関連の開発に対する成果ベースの根拠 として役⽴つものである。departmental bulletin pape

Helping behaviour during cooperative learning and learning gains

Is helping behaviour (i.e., solicited help and peer tutoring) during cooperative learning (CL) related to subsequent learning gains? And can teachers influence pupils’ helping behaviour? One hundred one 5th grade pupils from multiethnic schools, 10-12 years old, participated in the study. Forty two pupils (31 immigrant) worked in an experimental condition, characterized by the stimulation of solicited high quality help and 59 (24 immigrant) worked in a control condition. It was found that learning gains were predicted positively by pupils’ unsolicited helping behaviour (i.e., peer tutoring) and negatively by solicited help. Furthermore, teachers were able to affect pupils’ low quality solicited help only. Lastly, immigrant pupils used less helping behaviour than local pupils, irrespective of CL setting

Balancing Work and Family: A Controlled Evaluation of the Triple P – Positive Parenting Program as a Work-Site Intervention

Despite a wealth of evidence showing that behavioural family intervention is an effective intervention for parents of children with behavioural and emotional problems, little attention has been given to the relationship between parents functioning at work and their capacity to manage parenting and other home responsibilities. This study evaluated the effects of a group version of the Triple-P Positive Parenting Program (WPTP) designed specifically for delivery in the workplace.Participants were 42 general and academic staff from a major metropolitan university who were reporting difficulties managing home and work responsibilities and behavioural difficulties with their children. Participants were randomly assigned to WPTP, or to a waitlist control (WL) condition.Following intervention, parents in WPTP reported significantly lower levels of disruptive child behaviour, dysfunctional parenting practices, and higher levels of parental self-efficacy in managing both home and work responsibilities, than parents in the WL condition. These short-term improvements were maintained at 4-months follow-up. There were also additional improvements in reported levels of work stress and parental distress at follow-up in the WPTP group compared to post-intervention.Implications for the development of 'family-friendly' work environments and the prevention of child behaviour problems are discussed

Plasma-treated polyethylene as electrochemical mediator for enzymatic glucose sensors: Toward bifunctional glucose and dopamine sensors

"This is the peer reviewed version of the following article: Buendía JJ, Fabregat G, Castedo A, Llorca J, Alemán C. Plasma-treated polyethylene as electrochemical mediator for enzymatic glucose sensors: Toward bifunctional glucose and dopamine sensors. Plasma Process Polym. 2017;e1700133, which has been published in final form at https://doi.org/10.1002/ppap.201700133. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-ArchivingThe application of inert and insulating low density polyethylene (LDPE) in electrochemical detection is null. However, in a recent study it was found that reactive species formed onto the surface of plasma-treated LDPE and other polymers promote the electrocatalytic oxidation of dopamine. In this work, we examine the role of plasma-treated LDPE as mediator in enzymatic glucose biosensors based on Glucose oxidase and glass carbon substrate. Results indicate that plasma-induced changes facilitate the electrocommunication between the enzyme and the substrate. The chronoamperometric response of these sensors prove their bifunctionality since the oxidation of glucose to gluconolactone, which is catalyzed by the GOx, coexists with the oxidation of dopamine that is electrocatalytized by the plasma activated LDPE surface.Peer ReviewedPostprint (author's final draft

An Examination of Body Dissatisfaction and Media Exposure

The goal of the current study was to examine the relationship between muscle magazine consumption and body dissatisfaction. The study also examined the relationship between muscle magazine consumption and the amount of disparity between ideal and real body shape. Participants (N = 108) were recruited via study board, and also on a volunteer basis, from a mid-Western university with a population of 20,674 students. The first hypothesis stated that men with greater exposure to muscle magazines would indicate that their actual body shape falls further away from their ideal body shape, in terms of both muscularity and fat level, than men who read fewer magazines that focus on muscle building. The second hypothesis stated that men with greater exposure to muscle magazines would indicate higher levels of body dissatisfaction than men who read fewer magazines focused on muscle building. To evaluate the first hypothesis, a linear regression analysis was conducted to determine whether magazine consumption would predict the distance between real and ideal body shape, in terms of both fat and muscle content. Results did not support the first hypothesis and are not consistent with prior research that showed higher levels of muscle and fitness magazine consumption correlating positively with a greater drive for thinness and a greater drive for muscularity. Results did not support the second hypothesis: greater exposure to muscle magazines did not lead to greater levels of body dissatisfaction. Regression analysis found that there was no predictive relationship between muscle magazine consumption and body dissatisfaction or muscle magazine consumption and greater distance between ideal and real body shapes. Additional t-test analysis found that men who read muscle magazines actually indicated significantly lower levels of dissatisfaction with their bodies than men who did not read muscle magazines; however, that result is restricted to collegiate athletes only. Results from this study are important in that they indicate, in men, there are other factors involved in determining how individuals end up with increased levels of body dissatisfaction. Prior findings also concluded that men’s concerns with body shape thrive as a combination of multiple constructs involving social, personal, and mass media factors. Although magazine literature has some bearing on body image concern, particularly with women, it is unclear as to whether magazine consumption contributes to its effect on men. One limitation of this study is that participants reported on the degree to which they were dissatisfied with their bodies. Some individuals may not have acknowledged their level of dissatisfaction with their own bodies. Information based upon self-reporting measures may have been inaccurate due to under or over reporting in surveys. Another limitation of this study, particularly when using the results as a comparison tool toward previous studies, is that the measures used to determine levels of body dissatisfaction differ. Researchers abroad have used different measures to arrive at what would be considered similar conclusions regarding body dissatisfaction. There are multiple measures that can be used to determine a person’s level of satisfaction and drive for muscularity. Some measures are better than others at capturing variable characteristics. Another important limitation is that muscle magazines only represent one form of media consumption. A study that incorporated other forms of media may be more fruitful in its findings. Therefore, results should be interpreted with caution. However, with everexpanding information technology capabilities, society is increasingly bombarded with information more so than ever before. This study provides additional insight into the question of whether or not more is better. It also identifies a gender difference in the predicting power of magazine consumption and body dissatisfaction

Continuous PDE Dynamics Forecasting with Implicit Neural Representations

Effective data-driven PDE forecasting methods often rely on fixed spatial and / or temporal discretizations. This raises limitations in real-world applications like weather prediction where flexible extrapolation at arbitrary spatiotemporal locations is required. We address this problem by introducing a new data-driven approach, DINo, that models a PDE's flow with continuous-time dynamics of spatially continuous functions. This is achieved by embedding spatial observations independently of their discretization via Implicit Neural Representations in a small latent space temporally driven by a learned ODE. This separate and flexible treatment of time and space makes DINo the first data-driven model to combine the following advantages. It extrapolates at arbitrary spatial and temporal locations; it can learn from sparse irregular grids or manifolds; at test time, it generalizes to new grids or resolutions. DINo outperforms alternative neural PDE forecasters in a variety of challenging generalization scenarios on representative PDE systems