Interrelationship between contractility, protein synthesis and metabolism in mantle of juvenile cuttlefish (Sepia officinalis)

Young juvenile cuttlefish (Sepia officinalis) can grow at rates as high as 12% body weight per day. How the metabolic demands of such a massive growth rate impacts muscle performance that competes for ATP is unknown. Here, we integrate aspects of contractility, protein synthesis, and energy metabolism in mantle of specimens weighing 1.1 g to lend insight into the processes. Isolated mantle muscle preparations were electrically stimulated and isometric force development monitored. Preparations were forced to contract at 3 Hz for 30 s to simulate a jetting event. We then measured oxygen consumption, glucose uptake and protein synthesis in the hour following the stimulation. Protein synthesis was inhibited with cycloheximide and glycolysis was inhibited with iodoacetic acid in a subset of samples. Inhibition of protein synthesis impaired contractility and decreased oxygen consumption. An intact protein synthesis is required to maintain contractility possibly due to rapidly turning over proteins. At least, 41% of whole animal ṀO2 is used to support protein synthesis in mantle, while the cost of protein synthesis (50 μmol O2 mg protein-1) in mantle was in the range reported for other aquatic ectotherms. A single jetting challenge stimulated protein synthesis by approximately 25% (2.51-3.12% day-1) over a 1 h post contractile period, a similar response to that which occurs in mammalian skeletal muscle. Aerobic metabolism was not supported by extracellular glucose leading to the contention that at this life stage either glycogen or amino acids are catabolized. Regardless, an intact glycolysis is required to support contractile performance and protein synthesis in resting muscle. It is proposed that glycolysis is needed to maintain intracellular ionic gradients. Intracellular glucose at approximately 3 mmol L-1 was higher than the 1 mmol L-1 glucose in the bathing medium suggesting an active glucose transport mechanism. Octopine did not accumulate during a single physiologically relevant jetting challenge; however, octopine accumulation increased following a stress that is sufficient to lower Arg-P and increase free arginine.Agência financiadora Natural Sciences and Engineering Research Council of Canada (NSERC) NSERC CPB section of the Canadian Society of Zoologists Fundacao para a Ciencia e a Tecnologia (FCT) IF/00576/2014 Portuguese national funds from Programa Operacional Mar2020 (Portugal2020/FEAMP) - Project SEPIACUL 16-02-01-FMP-53 FCT through Plurennial UID/Multi/04326/2019 EMBRC. PT ALG-01-0145-FEDER-022121 Portuguese node of EMBRC-ERICinfo:eu-repo/semantics/publishedVersio

Vaccination and Small-Pox in Japan

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Leadership Strategies for Reducing Medical Errors

Medical errors can be preventable with ongoing education and training. However, some health care leaders struggle to develop and implement new strategies for reducing medical errors. Healthcare leaders must develop and implement new strategies to reduce errors, as medical errors may lead to a decrease in patients seeking medical services, financial liability, and a lack of community trust. Grounded in transformational leadership theory and the define, measure, analyze, improve, and control process, the purpose of this qualitative single case study was to explore leadership strategies for reducing medical errors. The participants included six healthcare leaders with 5 years of experience at a single hospital in Southern California. Data were collected using semistructured interviews. Thematic analysis resulted in the emergence of five common themes: (a) team collaboration; (b) team communication; (c) coaching, education, and training; (d) performance improvement; and (e) leadership. A key recommendation is for health care leaders to include healthcare workers in the feedback loop when developing new strategies and introducing new product lines and processes. The implication for positive social change includes the potential for higher quality care, reduced medical errors, and increased community trust

Leadership Strategies for Reducing Medical Errors

Medical errors can be preventable with ongoing education and training. However, some health care leaders struggle to develop and implement new strategies for reducing medical errors. Healthcare leaders must develop and implement new strategies to reduce errors, as medical errors may lead to a decrease in patients seeking medical services, financial liability, and a lack of community trust. Grounded in transformational leadership theory and the define, measure, analyze, improve, and control process, the purpose of this qualitative single case study was to explore leadership strategies for reducing medical errors. The participants included six healthcare leaders with 5 years of experience at a single hospital in Southern California. Data were collected using semistructured interviews. Thematic analysis resulted in the emergence of five common themes: (a) team collaboration; (b) team communication; (c) coaching, education, and training; (d) performance improvement; and (e) leadership. A key recommendation is for health care leaders to include healthcare workers in the feedback loop when developing new strategies and introducing new product lines and processes. The implication for positive social change includes the potential for higher quality care, reduced medical errors, and increased community trust

Automatic Discrimination between Scomber japonicus and Scomber australasicus by Geometric and Texture Features

This paper proposes a method for automatic discrimination of two mackerel species: Scomber japonicus (chub mackerel) and Scomber australasicus (blue mackerel). Because S. japonicus has a much higher market price than S. australasicus, the two species must be properly sorted before shipment, but their similar appearance makes discrimination difficult. These species can be effectively distinguished using the ratio of the base length between the dorsal fin’s first and ninth spines to the fork length. However, manual measurement of this ratio is time-consuming and reduces fish freshness. The proposed technique instead uses image processing to measure these lengths. We were able to successfully discriminate between the two species using the ratio as a geometric feature, in combination with several texture features. We then quantitatively verified the effectiveness of the proposed method and demonstrated that it is highly accurate in classifying mackerel

Triptolide, an active compound identified in a traditional Chinese herb, induces apoptosis of rheumatoid synovial fibroblasts

BACKGROUND: Extracts of Tripterygium wilfordii Hook F (TWHF), a traditional Chinese herb, have been reported to show efficacy in patients with rheumatoid arthritis (RA). Since RA is not only characterized by inflammation but also by synovial proliferation in the joints, we examined whether triptolide (a constituent of TWHF) could influence the proliferation of rheumatoid synovial fibroblasts (RSF) by induction of apoptosis. RESULTS: RSF were obtained from RA patients during surgery and were treated with triptolide under various conditions. The viability and proliferation of RSF were measured by the 4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate (WST-1) assay and by 5-bromo-2'-deoxyuridine incorporation, respectively. Apoptosis was identified by detection of DNA fragmentation using an enzyme-linked immunosorbent assay and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL). The role of caspases in apoptosis of RSF was analyzed by measuring caspase-3 activity. Activation of the peroxisome proliferator-activated receptor (PPAR) γ was assessed by a luciferase reporter gene assay using RSF transfected with a plasmid containing the peroxisome proliferator response element. Triptolide decreased viability, inhibited proliferation, and induced apoptosis of RSF in a concentration-dependent manner at very low (nM) concentrations. Caspase-3 activity was increased by treatment with triptolide and was suppressed by caspase inhibitors. Although PPARγ activation was induced by 15-deoxy-Δ(12,14)-prostaglandin J(2), triptolide did not induce it under the same experimental conditions. An extract of TWHF also induced DNA fragmentation in RSF. CONCLUSION: The mechanism of action remains to be studied; however, triptolide may possibly have a disease-modifying effect in patients with RA