The role of dendritic cells in the development of graft versus host disease

Available from British Library Document Supply Centre-DSC:DXN052475 / BLDSC - British Library Document Supply CentreSIGLEGBUnited Kingdo

A Panel of Four Anti-HSPG Monoclonal Antibodies Benefits in Increasing the Specificity in Detection of Colorectal Cancer

Colorectal cancer (CRC) is the second leading with main cause of death is liver and lung metastasis. Using of a combination of genetic and epigenetic markers are addressed but the results have not been approved in clinical practice. A set of serum biomarkers has been proposed to increase accuracy in early diagnosis of CRC. In addition, non-invasive as well as the best prognostic panel of biomarkers and define predictive biomarkers for treatment of CRC are all aims of future research. HSPGs is an important biomolecule involving in cancer cell proliferation, differentiation, and migration. Membrane HSPGs shed into blood circulation and matrix in particular circumstance can be used as a specific biomarker for some cancer cells. In order to evaluate the benefit of a panel of anti-HSPGs monoclonal antibodies in increasing specificity to detect CRC, four clones of anti-HSPGs were studied for its specific reaction on various tumor cell lines by indirect immunofluorescent technique and analyzed by flow cytometer compared to normal white blood cells. A combination of two or more clones were focused. The results showed that all four clones presented a variation in reaction to all solid tumor cell lines tested but negative to normal white blood cells from different ABO blood groups. Interestingly, amongst those cells tested, HT29, a colorectal cancer cell lines were significantly reacted with all four monoclonal antibodies. Taken together, we proposed a panel of four anti-HSPGs monoclonal antibodies to be applied in various detection platforms to increase the specificity in screening of CRC. Keywords: Cancer biomarkers, Colorectal cancer, HSPG</jats:p

Effects of ascorbic acid supplementation on immune status in healthy women following a single bout of exercise

Abstract Background: This study was designed to determine the effects of ascorbic acid (AA) supplementation on immune status following a single bout of exercise. Materials/Methods: In a crossover design with a 1 week wash-out period, 20 healthy sedentary women performed 30 minutes moderate-intensity cycling with (1,000AA) or without (0AA) ingesting 1,000 mg of AA daily for 1 week. Blood samples were taken immediately before, immediately after and 24 hours post-exercise to determine the oxidative stress markers, hematological parameters, immunophenotyping of peripheral blood lymphocytes and neutrophil phagocytic function with Candida albicans. Results: Moderate-intensity exercise in subjects ranged in age from 21 to 23 years, showed no significant changes in oxidative stress markers in both cohorts. Plasma total creatine kinase was increased immediately after exercise and returned to baseline at 24 h post-exercise in both cohorts. Subjects ingesting 1,000 mg AA demonstrated significant higher level of plasma AA at pre-exercise and post-exercise as compared with the same time point in 0AA group. White blood cell and absolute neutrophil counts were increased immediately after exercise and returned to baseline at 24 h post-exercise in both cohorts. Exercise resulted in increased lymphocyte count, CD4+ T cells, CD8+ T cells, CD45+/CD3-/CD4- cells, CD45+/CD3+/CD4- cells, CD45+/CD3-/CD8- cells, CD45+/CD3+/CD8- cells and CD45+/CD3-/CD8+ cells immediately after exercise (p&lt;0.05) with a return to baseline at 24 hours in 0AA group. AA supplement mitigated effects of exercise on CD4+ T cells and CD45+/CD3-/CD8- cells. No significant change in neutrophil phagocytic function were observed when incubated with low or high concentrations of C. albicans in both cohorts. Conclusion: A single bout of exercise induced muscle damage and transient changes in neutrophil count as well as lymphocyte subpopulations in sedentary women. Ascorbic supplementation does not show beneficial effect to the moderate-intensity exercise.</jats:p