Loss of APC induces polyploidy as a result of a combination of defects in mitosis and apoptosis

Mutations in the adenomatous polyposis coli (APC) tumor suppressor gene initiate a majority of colorectal cancers. Acquisition of chromosomal instability is an early event in these tumors. We provide evidence that the loss of APC leads to a partial loss of interkinetochore tension at metaphase and alters mitotic progression. Furthermore, we show that inhibition of APC in U2OS cells compromises the mitotic spindle checkpoint. This is accompanied by a decrease in the association of the checkpoint proteins Bub1 and BubR1 with kinetochores. Additionally, APC depletion reduced apoptosis. As expected from this combination of defects, tetraploidy and polyploidy are consequences of APC inhibition in vitro and in vivo. The removal of APC produced the same defects in HCT116 cells that have constitutively active β-catenin. These data show that the loss of APC immediately induces chromosomal instability as a result of a combination of mitotic and apoptotic defects. We suggest that these defects amplify each other to increase the incidence of tetra- and polyploidy in early stages of tumorigenesis

The First Data Release from SweetSpot: 74 Supernovae in 36 Nights on WIYN+WHIRC

SweetSpot is a three-year National Optical Astronomy Observatory (NOAO) Survey program to observe Type Ia supernovae (SNe Ia) in the smooth Hubble flow with the WIYN High-resolution Infrared Camera (WHIRC) on the WIYN 3.5-m telescope. We here present data from the first half of this survey, covering the 2011B-2013B NOAO semesters, and consisting of 493 calibrated images of 74 SNe Ia observed in the rest-frame near-infrared (NIR) from $0.02 < z < 0.09$. Because many observed supernovae require host galaxy subtraction from templates taken in later semesters, this release contains only the 186 NIR ($JHK_s$) data points for the 33 SNe Ia that do not require host-galaxy subtraction. The sample includes 4 objects with coverage beginning before the epoch of B-band maximum and 27 beginning within 20 days of B-band maximum. We also provide photometric calibration between the WIYN+WHIRC and Two-Micron All Sky Survey (2MASS) systems along with light curves for 786 2MASS stars observed alongside the SNe Ia. This work is the first in a planned series of three SweetSpot Data Releases. Future releases will include the full set of images from all 3 years of the survey, including host-galaxy reference images and updated data processing and host-galaxy reference subtraction. SweetSpot will provide a well-calibrated sample that will help improve our ability to standardize distance measurements to SNe Ia, examine the intrinsic optical-NIR colors of SNe Ia at different epochs, explore nature of dust in other galaxies, and act as a stepping stone for more distant, potentially space-based surveys.Comment: Published in AJ. 10 tables. 11 figures. Lightcurve plots included as a figureset and available in source tarball. Data online at http://www.phyast.pitt.edu/~wmwv/SweetSpot/DR1_data

The First Data Release from SweetSpot: 74 Supernovae in 36 Nights on WIYN+WHIRC

SweetSpot is a three-year National Optical Astronomy Observatory (NOAO) Survey program to observe Type Ia supernovae (SNe Ia) in the smooth Hubble flow with the WIYN High-resolution Infrared Camera (WHIRC) on the WIYN 3.5-m telescope. We here present data from the first half of this survey, covering the 2011B-2013B NOAO semesters, and consisting of 493 calibrated images of 74 SNe Ia observed in the rest-frame near-infrared (NIR) from $0.02 < z < 0.09$. Because many observed supernovae require host galaxy subtraction from templates taken in later semesters, this release contains only the 186 NIR ($JHK_s$) data points for the 33 SNe Ia that do not require host-galaxy subtraction. The sample includes 4 objects with coverage beginning before the epoch of B-band maximum and 27 beginning within 20 days of B-band maximum. We also provide photometric calibration between the WIYN+WHIRC and Two-Micron All Sky Survey (2MASS) systems along with light curves for 786 2MASS stars observed alongside the SNe Ia. This work is the first in a planned series of three SweetSpot Data Releases. Future releases will include the full set of images from all 3 years of the survey, including host-galaxy reference images and updated data processing and host-galaxy reference subtraction. SweetSpot will provide a well-calibrated sample that will help improve our ability to standardize distance measurements to SNe Ia, examine the intrinsic optical-NIR colors of SNe Ia at different epochs, explore nature of dust in other galaxies, and act as a stepping stone for more distant, potentially space-based surveys.Comment: Published in AJ. 10 tables. 11 figures. Lightcurve plots included as a figureset and available in source tarball. Data online at http://www.phyast.pitt.edu/~wmwv/SweetSpot/DR1_data

Ageing, physical function, and the diurnal rhythms of cortisol and dehydroepiandrosterone

The present study examined the relationship between ageing, physical function and the diurnal rhythms of cortisol and dehydroepiandrosterone (DHEA). Participants were 36 community dwelling older adults aged between 65-86 years old. Salivary cortisol and DHEA were measured over the course of one day: immediately upon awakening, 30 min later, and then 3 h, 6 h, 9 h and 12 h post-awakening. Participants completed the Nottingham extended activities of daily living index, the Berg Balance Scale and their handgrip strength was assessed. Older participants had a significantly higher cortisol area under the curve (AUC), lower overall DHEA levels, lower DHEA AUC, a decreased diurnal slope of decline and increased cortisol:DHEA ratio. Lower diurnal cortisol levels were associated with poorer performance on the Berg Balance Scale and lower handgrip strength, and those with a flattened DHEA diurnal profile reported less independence in carrying out daily tasks. These associations withstood adjustment for age. In conclusion, this study suggests an association between cortisol, DHEA, ageing and physical function

Benefits of Promoting College Awareness for Low Income Middle and High School Students

The disadvantages that low-income students face in today’s society make it difficult for them to move on to a post-secondary education. Since higher education is no longer for the elite few and the number of students attending colleges and universities is rapidly increasing, all students need the necessary resources to prepare for a college ready future. In order to reach low-income students and close the learning gap between low, middle, and upper income peers, there will be a need for college awareness programs in the high poverty areas. This capstone examines the barriers low-income students face and how these college awareness programs develop and promote equal opportunity to benefit the students that they target

Habitat Use of Shovelnose Sturgeon Scaphirhynchus Platorynchus in the Lower Mississippi River

The habitat requirements of shovelnose sturgeon populations are unknown for the lower Mississippi River. Active acoustic telemetry was used to measure temporal variation in habitat use of shovelnose sturgeon and preference for depth, surface current velocity, and riverbed rugosity and slope by the population and within sandbar microhabitats. Shovelnose sturgeon occupied habitats differently throughout the year; and, within habitats, areas of moderate depth and surface current velocity and smooth riverbed were preferred. Within sandbars, environmental conditions did not differ from the surrounding environment, yet frequent aggregations of individuals in the lower portion of sandbars often in close proximity suggest habitat preference at a scale greater than the 5 ha measured. Results of this study provide information on seasonal habitat use patterns and methods that can be applied to a long-term dataset to identify the habitat requirements of shovelnose sturgeon