Découverte de cadres sémantiques dans le domaine de l'environnement: le cas de l'influence objective

Aquest article descriu una metodologia de descoberta de «marcs semàntics» (o frames, segons Fillmore) en un àmbit d'especialitat. Els principis que han guiat el nostre treball s'inspiren àmpliament en la semàntica de marcs (Fillmore, 1982; Fillmore i Baker, 2010) i en la seva aplicació en el recurs concebut per a l'anglès, és a dir FrameNet (Ruppenhofer et al., 2010). La nostra metodologia s'aplica a termes de natura predicativa (verbs, noms predicatius i adjectius) de l'àmbit del medi ambient. En aquest cas, ens centrem en els termes en anglès i francès que evoquen el marc d'influència objectiva (Objective_influence).This paper presents a methodology for discovering semantic frames in a specialized subject field. The principles on which it is based are derived from the Frame Semantics framework (Fillmore 1982; Fillmore and Baker 2010) and its application in the resource designed for English, i.e. FrameNet (Ruppenhofer et al. 2010). Our method is applied to predicative terms (verbs, predicative nouns and adjectives) used in the environment field The focus is on English and French terms that evoke the Objective_ influence frame

Propidium monoazide (PMA) and ethidium bromide monoazide(EMA) improve DNA array and high-throughput sequencing ofporcine reproductive and respiratory syndrome virus identification

Pan-viral DNA array (PVDA) and high-throughput sequencing (HTS) are useful tools to identify novel viruses of emerging diseases. However, both techniques have difficulties to identify viruses in clinical samples because of the host genomic nucleic acid content (hg/cont). Both propidium monoazide (PMA) and ethidium bromide monoazide (EMA) have the capacity to bind free DNA/RNA, but are cell membrane-impermeable. Thus, both are unable to bind protected nucleic acid such as viral genomes within intact virions. However, EMA/PMA modified genetic material cannot be amplified by enzymes. In order to assess the potential of EMA/PMA to lower the presence of amplifiable hg/cont in samples and improve virus detection, serum and lung tissue homogenates were spiked with porcine reproductive and respiratory virus (PRRSV) and were processed with EMA/PMA. In addition, PRRSV RT-qPCR positive clinical samples were also tested. EMA/PMA treatments significantly decreased amplifiable hg/cont and significantly increased the number of PVDA positive probes and their signal intensity compared to untreated spiked lung samples. EMA/PMA treatments also increased the sensitivity of HTS by increasing the number of specific PRRSV reads and the PRRSV percentage of coverage. Interestingly, EMA/PMA treatments significantly increased the sensitivity of PVDA and HTS in two out of three clinical tissue samples. Thus, EMA/PMA treatments offer a new approach to lower the amplifiable hg/cont in clinical samples and increase the success of PVDA and HTS to identify viruses

Combining the bulk transfer formulation and surface renewal analysis for estimating the sensible heat flux without involving the parameter KB-1

The single‐source bulk transfer formulation (based on the Monin‐Obukhov Similarity Theory, MOST) has been used to estimate the sensible heat flux, H, in the framework of remote sensing over homogeneous surfaces (HMOST). The latter involves the canopy parameter, , which is difficult to parameterize. Over short and dense grass at a site influenced by regional advection of sensible heat flux, HMOST with  = 2 (i.e., the value recommended) correlated strongly with the H measured using the Eddy Covariance, EC, method, HEC. However, it overestimated HEC by 50% under stable conditions for samples showing a local air temperature gradient larger than the measurement error, 0.4 km−1. Combining MOST and Surface Renewal analysis, three methods of estimating H that avoid dependency have been derived. These new expressions explain the variability of H versus , where is the friction velocity, is the radiometric surface temperature, and is the air temperature at height, z. At two measurement heights, the three methods performed excellently. One of the methods developed required the same readily/commonly available inputs as HMOST due to the fact that the ratio between and the ramp amplitude was found fairly constant under stable and unstable cases. Over homogeneous canopies, at a site influenced by regional advection of sensible heat flux, the methods proposed are an alternative to the traditional bulk transfer method because they are reliable, exempt of calibration against the EC method, and are comparable or identical in cost of application. It is suggested that the methodology may be useful over bare soil and sparse vegetation.This research was funded by CERESS project AGL2011–30498 (Ministerio de Economía y Competitividad of Spain, cofunded FEDER), CGL2012–37416‐C04‐01 (Ministerio de Ciencia y Innovación of Spain), and CEI Iberus, 2014 (Proyecto financiado por el Ministerio de Educación en el marco del Programa Campus de Excelencia Internacional of Spain)