Limitations of variable number of tandem repeat typing identified through whole genome sequencing of Mycobacterium avium subsp. paratuberculosis on a national and herd level

Background: Mycobacterium avium subsp. paratuberculosis (MAP), the causative bacterium of Johne’s disease in dairy cattle, is widespread in the Canadian dairy industry and has significant economic and animal welfare implications. An understanding of the population dynamics of MAP can be used to identify introduction events, improve control efforts and target transmission pathways, although this requires an adequate understanding of MAP diversity and distribution between herds and across the country. Whole genome sequencing (WGS) offers a detailed assessment of the SNP-level diversity and genetic relationship of isolates, whereas several molecular typing techniques used to investigate the molecular epidemiology of MAP, such as variable number of tandem repeat (VNTR) typing, target relatively unstable repetitive elements in the genome that may be too unpredictable to draw accurate conclusions. The objective of this study was to evaluate the diversity of bovine MAP isolates in Canadian dairy herds using WGS and then determine if VNTR typing can distinguish truly related and unrelated isolates.<p></p> Results: Phylogenetic analysis based on 3,039 SNPs identified through WGS of 124 MAP isolates identified eight genetically distinct subtypes in dairy herds from seven Canadian provinces, with the dominant type including over 80% of MAP isolates. VNTR typing of 527 MAP isolates identified 12 types, including “bison type” isolates, from seven different herds. At a national level, MAP isolates differed from each other by 1–2 to 239–240 SNPs, regardless of whether they belonged to the same or different VNTR types. A herd-level analysis of MAP isolates demonstrated that VNTR typing may both over-estimate and under-estimate the relatedness of MAP isolates found within a single herd.<p></p> Conclusions: The presence of multiple MAP subtypes in Canada suggests multiple introductions into the country including what has now become one dominant type, an important finding for Johne’s disease control. VNTR typing often failed to identify closely and distantly related isolates, limiting the applicability of using this typing scheme to study the molecular epidemiology of MAP at a national and herd-level.<p></p&gt

Worst of the bunch: visual comparative benchmarks change evaluations of government performance

Benchmarking theories argue voters use information about other countries’ performances, usually on the economy and obtained through experience or media, to evaluate their own governments. Yet existing observational evidence is relatively fragile and struggles to distinguish how people become more knowledgeable. Using a pre-registered experiment, we showed UK respondents a chart displaying the UK’s exceptionally high cumulative COVID-19 deaths either in isolation or alongside European countries with fewer deaths. Mimicking widely-circulated charts, this visual treatment enhances our study’s external validity and tests the media-based channel for benchmarking. Aligned with pre-registered expectations, seeing the UK as “worst of the bunch” compared to UK-only data caused more negative government evaluations. Unexpectedly, partisanship did not moderate the information effects, while exploratory tests revealed the visuals generated more negative evaluations among respondents with high political trust. Our study shows international comparisons in visual forms can change domestic opinion, and on matters beyond strictly economic performance

As we like it: did the UK's 2016 EU referendum reveal the "will of the people?"

Rhetoric surrounding the United Kingdom’s 2016 referendum on continued European Union (EU) membership frequently has invoked the “will of the people.” Addressing the House of Commons in March 2019, then–Prime Minster Theresa May stated that “my sense of responsibility and duty has meant that I have kept working to ensure that we deliver on the result and the will of the people” (March 27, 2019).1 May’s successor, Boris Johnson, appealed to the same notion when suggesting in the Daily Telegraph (September 15, 2019) that opposition parties were “united in wanting to cancel the referendum result…and overturn the will of the people.” On the other side of the debate, Caroline Lucas (currently the sole Member of Parliament for the UK’s Green Party) stated that “[e]very recent opinion poll shows that the will of the people has changed since [the referendum]” (December 4, 2018)

Exploring Protein–Nanoparticle Interactions with Coarse‐Grained Protein Folding Models

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/136668/1/smll201603748.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/136668/2/smll201603748-sup-0001-S1.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/136668/3/smll201603748_am.pd

Determinants of participation in a web-based health risk assessment and consequences for health promotion programs

Background: The health risk assessment (HRA) is a type of health promotion program frequently offered at the workplace. Insight into the underlying determinants of participation is needed to evaluate and implement these interventions. Objective: To analyze whether individual characteristics including demographics, health behavior, self-rated health, and work-related factors are associated with participation and nonparticipation in a Web-based HRA. Methods: Determinants of participation and nonparticipation were investigated in a cross-sectional study among individuals employed at five Dutch organizations. Multivariate logistic regression was performed to identify determinants of participation and nonparticipation in the HRA after controlling for organization and all other variables. Results: Of the 8431 employees who were invited, 31.9% (2686/8431) enrolled in the HRA. The online questionnaire was completed by 27.2% (1564/5745) of the nonparticipants. Determinants of participation were some periods of stress at home or work in the preceding year (OR 1.62, 95% CI 1.08-2.42), a decreasing number of weekdays on which at least 30 minutes were spent on moderate to vigorous physical activity (ORdayPA0.84, 95% CI 0.79-0.90), and increasing alcohol consumption. Determinants of nonparticipation were less-than-positive self-rated health (poor/very poor vs very good, OR 0.25, 95% CI 0.08-0.81) and tobacco use (at least weekly vs none, OR 0.65, 95% CI 0.46-0.90). Conclusions: This study showed that with regard to isolated health behaviors (insufficient physical activity, excess alcohol consumption, and stress), those who could benefit most from the HRA were more likely to participate. However, tobacco users and those who rate

The effects of divorce in a society with ever-changing family structures

Divorce rates have doubled since the 1970s, this has influenced both the structure and the impact of the family. It is imperative not to overlook the large and growing population of children affected by divorce. This paper will discuss the differences of important variables involved, such as age, gender and cultural background. In addition, it will discuss in detail outreach ideas and intervention strategies including small groups, mentoring programs, and bibliotherapy that have all been effective in supporting these children and their families in this crucial change of family structure. Children in single parent and blended families are considered at-risk because research shows these children are at a high risk for veering off the path of becoming productive adults who contribute positively to society (Anderson, 2002). School counselors and faculty are at times the only advocates for children when parents are distracted with working on their own issues that are brought on by the divorce. A school can provide a neutral environment, allowing continued involvement by both parents in their child\u27s life, which promotes academic success. School counselors can act as advocates because of their continued involvement, their knowledge of developmental needs, and their training in interventions that can reduce at-risk behavior

Proactive and politically skilled professionals: What is the relationship with affective occupational commitment?

The aim of this study is to extend research on employee affective commitment in three ways: (1) instead of organizational commitment the focus is on occupational commitment; (2) the role of proactive personality on affective occupational commitment is examined; and (3) occupational satisfaction is examined as a mediator and political skills as moderator in the relationship between proactive personality and affective occupational commitment. Two connected studies, one in a hospital located in the private sector and one in a university located in the public sector, are carried out in Pakistan, drawing on a total sample of over 400 employees. The results show that proactive personality is positively related to affective occupational commitment, and that occupational satisfaction partly mediates the relationship between proactive personality and affective occupational commitment. No effect is found for a moderator effect of political skills in the relationship between proactive personality and affective occupational commitment. Political skills however moderate the relationship between proactive personality and affective organizational commitment

Human Rights in the Context of Environmental Conservation on the US-Mexico Border

At Cabeza Priesta National Wildlife Refuge, a wilderness area on the US-Mexico border in Arizona, conflicting policies permit the provision of supplementary water for wildlife but not for undocumented immigrants passing through the area. Federal refuge environmental policy prioritizes active management of endangered and threatened species. Vast systems of water resources have been developed to support wildlife conservation in this extremely hot and dry environment. At the same time, humanitarian groups are not allowed to supply water to undocumented border crossers in the park. Human border-crossers must utilize non-potable wildlife water guzzlers for survival and face risk of illness or death by dehydration. This article analyzes human rights via an ethnographic lens. From this perspective, water policy at the wildlife refuge brings into question the value of human life in a border conservation context, especially for those entering the site illegally