On the absolute age of the Globular Cluster M92

We present precise and deep optical photometry of the globular M92. Data were collected in three different photometric systems: Sloan Digital Sky Survey (g',r',i',z'; MegaCam@CFHT), Johnson-Kron-Cousins (B, V, I; various ground-based telescopes) and Advanced Camera for Surveys (ACS) Vegamag (F475W, F555W, F814W; Hubble Space Telescope). Special attention was given to the photometric calibration, and the precision of the ground-based data is generally better than 0.01 mag. We computed a new set of {\alpha}-enhanced evolutionary models accounting for the gravitational settling of heavy elements at fixed chemical composition ([{\alpha}/Fe]=+0.3, [Fe/H]=-2.32 dex, Y=0.248). The isochrones -- assuming the same true distance modulus ({\mu}=14.74 mag), the same reddening (E(B-V)=0.025+-0.010 mag), and the same reddening law -- account for the stellar distribution along the main sequence and the red giant branch in different Color-Magnitude Diagrams (i',g'-i' ; i',g'-r' ; i',g'-z' ; I,B-I ; F814W,F475W-F814W). The same outcome applies to the comparison between the predicted Zero-Age-Horizontal-Branch (ZAHB) and the HB stars. We also found a cluster age of 11 +/- 1.5 Gyr, in good agreement with previous estimates. The error budget accounts for uncertainties in the input physics and the photometry. To test the possible occurrence of CNO-enhanced stars, we also computed two sets of {\alpha}- and CNO-enhanced (by a factor of three) models both at fixed total metallicity ([M/H]=-2.10 dex) and at fixed iron abundance. We found that the isochrones based on the former set give the same cluster age (11 +/- 1.5 Gyr) as the canonical {\alpha}-enhanced isochrones. The isochrones based on the latter set also give a similar cluster age (10 +/- 1.5 Gyr). These indings support previous results concerning the weak sensitivity of cluster isochrones to CNO-enhanced chemical mixtures.Comment: This paper makes use of data obtained from the Isaac Newton Group Archive which is maintained as part of the CASU Astronomical Data Centre at the Institute of Astronomy, Cambridge. This research used the facilities of the Canadian Astronomy Data Centre operated by the National Research Council of Canada with the support of the Canadian Space Agenc

On the Delta V_HB_bump parameter in Globular Clusters

We present new empirical estimates of the Delta V_HB_bump parameter for 15 Galactic globular clusters (GGCs) using accurate and homogeneous ground-based optical data. Together with similar evaluations available in the literature, we ended up with a sample of 62 GGCs covering a very broad range in metal content (-2.16<=[M/H]<=-0.58 dex). Adopting the homogeneous metallicity scale provided either by Kraft & Ivans (2004) or by Carretta et al. (2009), we found that the observed Delta V_HB_bump parameters are larger than predicted. In the metal-poor regime ([M/H]<=-1.7, -1.6 dex) 40% of GCs show discrepancies of 2sigma (~0.40 mag) or more. Evolutionary models that account either for alpha- and CNO-enhancement or for helium enhancement do not alleviate the discrepancy between theory and observations. The outcome is the same if we use the new Solar heavy-element mixture. The comparison between alpha- and CNO-enhanced evolutionary models and observations in the Carretta et al. metallicity scale also indicates that observed Delta V_HB_bump parameters, in the metal-rich regime ([M/H]=>0), might be systematically smaller than predicted

Ions modulate stress-induced nano-texture in supported fluid lipid bilayers.

Most plasma membranes comprise a large number of different molecules including lipids and proteins. In the standard fluid mosaic model, the membrane function is effected by proteins whereas lipids are largely passive and serve solely in the membrane cohesion. Here we show, using supported 1,2-dioleoyl-sn-glycero-3-phosphocholine lipid bilayers in different saline solutions, that ions can locally induce ordering of the lipid molecules within the otherwise fluid bilayer when the latter is supported. This nanoordering exhibits a characteristic length scale of ∼20 nm, and manifests itself clearly when mechanical stress is applied to the membrane. Atomic force microscopy (AFM) measurements in aqueous solutions containing NaCl, KCl, CaCl2, and Tris buffer show that the magnitude of the effect is strongly ion-specific, with Ca2+ and Tris, respectively, promoting and reducing stress-induced nanotexturing of the membrane. The AFM results are complemented by fluorescence recovery after photobleaching experiments, which reveal an inverse correlation between the tendency for molecular nanoordering and the diffusion coefficient within the bilayer. Control AFM experiments on other lipids and at different temperatures support the hypothesis that the nanotexturing is induced by reversible, localized gel-like solidification of the membrane. These results suggest that supported fluid phospholipid bilayers are not homogenous at the nanoscale, but specific ions are able to locally alter molecular organization and mobility, and spatially modulate the membrane’s properties on a length scale of ∼20 nm. To illustrate this point, AFM was used to follow the adsorption of the membrane-penetrating antimicrobial peptide Temporin L in different solutions. The results confirm that the peptides do not absorb randomly, but follow the ion-induced spatial modulation of the membrane. Our results suggest that ionic effects have a significant impact for passively modulating the local properties of biological membranes, when in contact with a support such as the cytoskeleton

A multi-center study on the attitudes of Malaysian emergency health care staff towards allowing family presence during resuscitation of adult patients

BACKGROUND The practice of allowing family members to witness on-going active resuscitation has been gaining ground in many developed countries since it was first introduced in the early 1990s. In many Asian countries, the acceptability of this practice has not been well studied. AIM We conducted a multi-center questionnaire study to determine the attitudes of health care professionals in Malaysia towards family presence to witness ongoing medical procedures during resuscitation. METHODS Using a bilingual questionnaire (in Malay and English language), we asked our respondents about their attitudes towards allowing family presence (FP) as well as their actual experience of requests from families to be allowed to witness resuscitations. Multiple logistic regression was used to analyze the association between the many variables and a positive attitude towards FP. RESULTS Out of 300 health care professionals who received forms, 270 responded (a 90% response rate). Generally only 15.8% of our respondents agreed to allow relatives to witness resuscitations, although more than twice the number (38.5%) agreed that relatives do have a right to be around during resuscitation. Health care providers are significantly more likely to allow FP if the procedures are perceived as likely to be successful (e.g., intravenous cannulation and blood taking as compared to chest tube insertion). Doctors were more than twice as likely as paramedics to agree to FP (p-value = 0.002). This is probably due to the Malaysian work culture in our health care systems in which paramedics usually adopt a 'follow-the-leader' attitude in their daily practice. CONCLUSION The concept of allowing FP is not well accepted among our Malaysian health care providers

Diagnosis of acute aortic syndromes with ultrasound and D-dimer: the PROFUNDUS study

Background: In patients complaining common symptoms such as chest/abdominal/back pain or syncope, acute aortic syndromes (AAS) are rare underlying causes. AAS diagnosis requires urgent advanced aortic imaging (AAI), mostly computed tomography angiography. However, patient selection for AAI poses conflicting risks of misdiagnosis and overtesting. Objectives: We assessed the safety and efficiency of a diagnostic protocol integrating clinical data with point-of-care ultrasound (POCUS) and d-dimer (single/age-adjusted cutoff), to select patients for AAI. Methods: This prospective study involved 12 Emergency Departments from 5 countries. POCUS findings were integrated with a guideline-compliant clinical score, to define the integrated pre-test probability (iPTP) of AAS. If iPTP was high, urgent AAI was requested. If iPTP was low and d-dimer was negative, AAS was ruled out. Patients were followed for 30 days, to adjudicate outcomes. Results: Within 1979 enrolled patients, 176 (9 %) had an AAS. POCUS led to net reclassification improvement of 20 % (24 %/-4 % for events/non-events, P &lt; 0.001) over clinical score alone. Median time to AAS diagnosis was 60 min if POCUS was positive vs 118 if negative (P = 0.042). Within 941 patients satisfying rule-out criteria, the 30-day incidence of AAS was 0 % (95 % CI, 0-0.41 %); without POCUS, 2 AAS were potentially missed. Protocol rule-out efficiency was 48 % (95 % CI, 46-50 %) and AAI was averted in 41 % of patients. Using age-adjusted d-dimer, rule-out efficiency was 54 % (difference 6 %, 95 % CI, 4-9 %, vs standard cutoff). Conclusions: The integrated algorithm allowed rapid triage of high-probability patients, while providing safe and efficient rule-out of AAS. Age-adjusted d-dimer maximized efficiency. Clinical trial registration: Clinicaltrials.gov, NCT04430400

Au25(SCnH2 n+1)18 Clusters in Biomimetic Membranes: Role of Size, Charge, and Transmembrane Potential in Direct Membrane Permeation

Gold nanoclusters and nanoparticles are promising materials for applications in nanomedicine, and therefore, understanding their interaction with cell membranes is of particular importance. A series of neutral and anionic Au25(SCnH2n+1)18 monolayer protected clusters (MPCs) (briefly, Cn0 and Cn- clusters), was embedded into two types of biomimetic membranes supported by mercury electrodes. The first was a dioleoylphosphatidylcholine (DOPC) self-assembled monolayer (SAM), whereas the second was a tethered bilayer lipid membrane (tBLM) obtained by first anchoring a thiolipid monolayer to the mercury surface and then self-assembling a DOPC monolayer on top of it. The diameter of these clusters, from 1.7 to 2.7 nm depending on the thiolate ligand, is smaller than the thickness of biomembranes and biomimetic membranes. Both neutral and anionic Au25(SCnH2n+1)18 MPCs can penetrate the lipid bilayer moiety of the tBLM, without disrupting it; in particular, anionic Au25 clusters require positive transmembrane potentials to do so. Neutral Au25 clusters exchange one electron with mercury in a DOPC SAM, where they can come in contact with the mercury surface, whereas they are prevented from doing so at the tBLM because of their inability to cross the hydrophilic chain separating the lipid bilayer moiety from the mercury surface. The potential of these Au25 clusters to penetrate directly the plasma membrane is particularly convenient for targeted drug delivery. They are highly stable, biocompatible, and catalytic, and their uniform size is of importance in nanomedicine. Moreover, they may induce an efficient energy transfer to 3O2, allowing applications in radiotherapy and antimicrobial activity. \ua