Differences in metal sequestration between zebra mussels from clean and polluted field locations

Organisms are able to detoxify accumulated metals by, e.g. binding them to metallothionein (MT) and/or sequestering them in metal-rich granules (MRG). The different factors involved in determining the capacity or efficiency with which metals are detoxified are not yet known.In this work we studied how the sub-cellular distribution pattern of cadmium, copper and zinc in whole tissue of zebra mussels from clean and polluted surface waters is influenced by the total accumulated metal concentration and by its physiological condition. Additionally we measured the metallothionein concentration in the mussel tissue. Metal concentration increased gradually in the metal-sensitive and detoxified sub-cellular fractions with increasing whole tissue concentrations. However, metal concentrations in the sensitive fractions did not increase to the same extent as metal concentrations in whole tissues. In more polluted mussels the contribution of MRG and MT became more important. Nevertheless, metal detoxification was not sufficient to prevent metal binding to heat-sensitive low molecular weight proteins (HDP fraction). Finally we found an indication that metal detoxification was influenced by the condition of the zebra mussels. MT content could be explained for up to 83% by variations in Zn concentration and physiological condition of the mussels

Occurrence and profiles of PCBs and PBDEs in harbour seals and harbour porpoises from the southern North Sea

Harbour porpoises (Phocoena phocoen) and harbour seals (Phoca vitulina), two representative top coastal pollution. Concentrations of sum PCBs were 1-2 orders of magnitude higher than concentrations of sum PBDEs (with median values of 23.1 μg.g- lw (lipid weight) and 12.4 μ.g-1 lw for sum PCBs and 0.33 μ.g- lw and 0.76 μ.g-1 lw for sum PBDEs in harbour seals and harbour porpoises respectively) and were highly dependent of age group and gender. For both species, the highest PCB concentrations were observed in adult males as the result of accumulation for years and years, while the highest PBDE concentrations were measured in juveniles probably due to better developed metabolic capacities for these congeners with age in adults. Results for PCBs were higher than observations in harbour seals and porpoises from other areas, while results for PBDEs were comparable indicating that the North Sea is a highly contaminated area. Relative PCB and PBDE profiles were constructed to compare metabolic capacities between harbour seals and porpoises. A higher contribution of lower chlorinated and nonpersistent congeners, such as CB 52, CB 95, CB 101, CB 118 and CB 149 indicated that harbour porpoises are unable to metabolize these compounds. Similar to PCBs, higher contributions of other PBDEs than BDE 47 were observed in harbour porpoises, suggesting that this species has difficulties to metabolize these congeners. In contrast, harbour seals showed a higher ability to metabolize PCBs and PBDEs