8 research outputs found
Synergistic biodegradation of poly(ethylene terephthalate) using Microbacterium oleivorans and Thermobifida fusca cutinase
No full textDisplay of fungal hydrophobin on the Pichia pastoris cell surface and its influence on Candida antarctica lipase B
No full textA Comprehensive Phylogenetic and Bioinformatics Assessment of Hydrophobin Protein (HYPAI) for Drug Delivery: an In Silico Analysis
No full textSurface engineering of polyester-degrading enzymes to improve efficiency and tune specificity
No full textCertain members of the carboxylesterase superfamily can act at the interface between water and water-insoluble substrates. However, nonnatural bulky polyesters usually are not efficiently hydrolyzed. In the recent years, the potential of enzyme engineering to improve hydrolysis of synthetic polyesters has been demonstrated. Regions on the enzyme surface have been modified by using site-directed mutagenesis in order to tune sorption processes through increased hydrophobicity of the enzyme surface. Such modifications can involve specific amino acid substitutions, addition of binding modules, or truncation of entire domains improving sorption properties and/or dynamics of the enzyme. In this review, we provide a comprehensive overview on different strategies developed in the recent years for enzyme surface engineering to improve the activity of polyester-hydrolyzing enzymes
