Therapeutic efficacy of favipiravir against Bourbon virus in mice

Bourbon virus (BRBV) is an emerging tick-borne RNA virus in the orthomyxoviridae family that was discovered in 2014. Although fatal human cases of BRBV have been described, little is known about its pathogenesis, and no antiviral therapies or vaccines exist. We obtained serum from a fatal case in 2017 and successfully recovered the second human infectious isolate of BRBV. Next-generation sequencing of the St. Louis isolate of BRBV (BRBV-STL) showed >99% nucleotide identity to the original reference isolate. Using BRBV-STL, we developed a small animal model to study BRBV-STL tropism in vivo and evaluated the prophylactic and therapeutic efficacy of the experimental antiviral drug favipiravir against BRBV-induced disease. Infection of Ifnar1-/- mice lacking the type I interferon receptor, but not congenic wild-type animals, resulted in uniformly fatal disease 6 to 10 days after infection. RNA in situ hybridization and viral yield assays demonstrated a broad tropism of BRBV-STL with highest levels detected in liver and spleen. In vitro replication and polymerase activity of BRBV-STL were inhibited by favipiravir. Moreover, administration of favipiravir as a prophylaxis or as post-exposure therapy three days after infection prevented BRBV-STL-induced mortality in immunocompromised Ifnar1-/- mice. These results suggest that favipiravir may be a candidate treatment for humans who become infected with BRBV

Direct measurement of the pion valence quark momentum distribution, the pion light-cone wave function squared

We present the first direct measurements of the pion valence quark momentum distribution which is related to the square of the pion light-cone wave function. The measurements were carried out using data on diffractive dissociation of 500 GeV/c $\pi^-$ into di-jets from a platinum target at Fermilab experiment E791. The results show that the $|q\bar {q}>$ light-cone asymptotic wave function, which was developed using perturbative QCD methods, describes the data well for $Q^2 \sim 10 ~{\rm (GeV/c)^2}$ or more. We also measured the transverse momentum distribution of the diffractive di-jets.Comment: 13 pages, 4 figure

Study of the Ds+→π−π+π+D^+_s \to \pi^- \pi^+ \pi^+ decay and measurement of f0f_0 masses and widths

From a sample of 848 $\pm$ 44 $D_s^+ \to \pi^- \pi^+ \pi^+$ decays, we find $\Gamma(D_s^+ \to \pi^- \pi^+ \pi^+) / \Gamma(D_s^+ \to \phi \pi^+) = 0.245 \pm 0.028^{+0.019}_{-0.012}$. Using a Dalitz plot analysis of this three body decay, we find significant contributions from the channels $\rho^0(770)\pi^+$, $\rho^0(1450)\pi^+$, $f_0(980)\pi^+$, $f_2(1270)\pi^+$, and $f_0(1370)\pi^+$. We present also the values obtained for masses and widths of the resonances $f_0(980)$ and $f_0(1370)$.Comment: 10 pages, 3 eps figure

Search for the Flavor-Changing Neutral-Current Decays D+→π+μ+μ−D^+\to \pi^+ \mu^+ \mu^- and D+→π+e+e−D^+\to \pi^+ e^+ e^-

We report the results of a search for the flavor-changing neutral-current decays $D^+\rightarrow \pi^+ \mu^+ \mu^-$ and $D^+\rightarrow \pi^+ e^+ e^-$ in data from Fermilab charm hadroproduction experiment E791. No signal above background is found, and we obtain upper limits on branching fractions, $B(D^+\rightarrow \pi^+ \mu^+ \mu^-) < 1.8 \times 10^{-5}$ and $B(D^+\rightarrow \pi^+ e^+ e^-) < 6.6 \times 10^{-5}$, at the 90\% confidence level.Comment: nine pages with figures; compressed, uuencoded postscrip

Dalitz Plot Analysis of the Decay D^+ --> K^- pi^+ pi^+ and Indication of a Low-Mass Scalar K pi Resonance

We study the Dalitz plot of the decay D^+ --> K^- pi^+ pi^+ with a sample of 15090 events from Fermilab experiment E791. Modeling the decay amplitude as the coherent sum of known K pi resonances and a uniform nonresonant term, we do not obtain an acceptable fit. If we allow the mass and width of the K^*_0(1430) to float, we obtain values consistent with those from PDG but the chi^2 per degree of freedom of the fit is still unsatisfactory. A good fit is found when we allow for the presence of an additional scalar resonance, with mass 797 +/- 19 +/- 43 MeV/c^2 and width 410 +/- 43 +/- 87 MeV/c^2. The mass and width of the K^*_0(1430) become 1459 +/- 7 +/- 5 MeV/c^2 and 175 +/- 12 +/- 12 MeV/c^2, respectively. Our results provide new information on the scalar sector in hadron spectroscopy.Comment: Accepted for publication in Physical Review Letter

Quantification and Characterization of Aluminum Distributions in Commercial Beta and Mordenite Zeolites by Cobalt Exchange

The aluminum distribution throughout the zeolite framework determines the structural, ion-exchange and catalytic properties of the zeolite. Several methods have been proposed to control the Al distribution, but in order to accurately assess these methods a procedure is needed to quantify Al distribution in various zeolite frameworks. Co2+ ions exchange onto the zeolite framework at Al pairs, and atomic absorbance spectroscopy (AAS) can be used to quantify the number of exchanged Co2+ ions and, in turn, the overall number of Al pairs. Each framework exhibits differences in pore size and channel configuration which affect the equilibrium conditions needed for saturation of all paired Al sites with Co2+ ions. In order to achieve saturation of the Co2+ ions, a reproducible exchange procedure must be developed for each framework of interest. Commercial beta (BEA) and mordenite (MOR) zeolites were subjected to liquid-phase cobalt ion exchange with varying exchange solution molarity, temperature, number of repetitions and time of exchange. The zeolites were then washed and treated in an oxidizing environment at high temperatures before undergoing AAS analysis to determine Co2+ concentration and diffuse reflectance UV-Vis spectroscopy (DRUV-VIS) to ensure only bare Co2+ ions were present. The BEA framework was found to achieve saturation at the following conditions: 0.50 M Co(NO3)2 exchange solution, ambient temperature, 1 repetition and 12 hour exchange time. The exchange procedure for MOR zeolites requires a 0.05 M Co(NO3)2 solution, ambient temperature, 24 hour exchange time and 1 repetition. These procedures will aid in the creation of an accurate catalog of the Al distribution in various commercially available BEA and MOR zeolites, as well as aiding in further synthesis studies to control the Al distribution in BEA and MOR zeolites