On The Age Estimation of LBDS 53W091

The recent spectral analysis of LBDS 53W091 by Spinrad and his collaborators has suggested that this red galaxy at z=1.55 is at least 3.5 Gyr old. This imposes an important constraint on cosmology, suggesting that this galaxy formed at z > 6.5, assuming recent estimates of cosmological parameters. We have performed chi^2 tests to the continuum of this galaxy using its UV spectrum and photometric data (RJHK). We have used the updated Yi models that are based on the Yale tracks. We find it extremely difficult to reproduce such large age estimates, under the assumption of the most probable input parameters. Using the same configuration as in Spinrad et al. (solar abundance models), our analysis suggests an age of approximately 1.4 -- 1.8 Gyr. The discrepancy between Spinrad et al.'s age estimate (based on the 1997 Jimenez models) and ours originates from the large difference in the model integrated spectrum: the Jimenez models are much bluer than the Yi models and the Bruzual \& Charlot (BC) models. Preliminary tests favor the Yi and BC models. The updated age estimate of LBDS 53W091 would suggest that this galaxy formed approximately at z=2-3.Comment: LaTeX, 18 eps files Accepted for publication in ApJ (Feb 10, 2000, vol 530), uses emulateapj.st

Spores of Clostridium engineered for clinical efficacy and safety cause regression and cure of tumors in vivo.

Spores of some species of the strictly anaerobic bacteria Clostridium naturally target and partially lyse the hypoxic cores of tumors, which tend to be refractory to conventional therapies. The anti-tumor effect can be augmented by engineering strains to convert a non-toxic prodrug into a cytotoxic drug specifically at the tumor site by expressing a prodrug-converting enzyme (PCE). Safe doses of the favored prodrug CB1954 lead to peak concentrations of 6.3 μM in patient sera, but at these concentration(s) known nitroreductase (NTR) PCEs for this prodrug show low activity. Furthermore, efficacious and safe Clostridium strains that stably express a PCE have not been reported. Here we identify a novel nitroreductase from Neisseria meningitidis, NmeNTR, which is able to activate CB1954 at clinically-achievable serum concentrations. An NmeNTR expression cassette, which does not contain an antibiotic resistance marker, was stably localized to the chromosome of Clostridium sporogenes using a new integration method, and the strain was disabled for safety and containment by making it a uracil auxotroph. The efficacy of Clostridium-Directed Enzyme Prodrug Therapy (CDEPT) using this system was demonstrated in a mouse xenograft model of human colon carcinoma. Substantial tumor suppression was achieved, and several animals were cured. These encouraging data suggest that the novel enzyme and strain engineering approach represent a promising platform for the clinical development of CDEPT

F stars, metallicity, and the ages of red galaxies at z > 1

We explore whether the rest-frame near-UV spectral region, observable in high-redshift galaxies via optical spectroscopy, contains sufficient information to allow the degeneracy between age and metallicity to be lifted. We do this by testing the ability of evolutionary synthesis models to reclaim the correct metallicity when fitted to the near-UV spectra of F stars of known (sub-solar and super-solar) metallicity. F stars are of particular interest because the rest-frame near-UV spectra of the oldest known elliptical galaxies at z > 1 appear to be dominated by F stars near to the main-sequence turnoff. We find that, in the case of the F stars, where the HST ultraviolet spectra have high signal:noise, model-fitting with metallicity allowed to vary as a free parameter is rather successful at deriving the correct metallicity. As a result, the estimated turnoff ages of these stars yielded by the model fitting are well constrained. Encouraged by this we have fitted these same variable- metallicity models to the deep, optical spectra of the z \simeq 1.5 mJy radio galaxies 53W091 and 53W069 obtained with the Keck telescope. While the age-metallicity degeneracy is not so easily lifted for these galaxies, we find that even when metallicity is allowed as a free parameter, the best estimates of their ages are still \geq 3 Gyr, with ages younger than 2 Gyr now strongly excluded. Furthermore, we find that a search of the entire parameter space of metallicity and star formation history using MOPED (Heavens et al., 2000) leads to the same conclusion. Our results therefore continue to argue strongly against an Einstein-de Sitter universe, and favour a lambda-dominated universe in which star formation in at least these particular elliptical galaxies was completed somewhere in the redshift range z = 3 - 5.Comment: 10 pages, LaTeX, uses MNRAS style file, incorporates 14 postscript figures, submitted to MNRAS. Changes include: inclusion of single stellar atmosphere model fits; more rigorous calculation of confidence regions; some re-structurin

Mass fluxes for O stars

The theory of moving reversing layers for hot stars is updated to include an extensive line list, a radiative boundary condition from static model atmospheres, line transfer by scattering, and continuation to supersonic velocities. A Monte Carlo technique determines the theory's eigenvalue J, the mass flux, and the derived J's are in good agreement with the wind models of Pauldrach et al. (2001). The solutions' sensitivity to the photospheric microturbulent velocity reveals that this parameter has a throttling effect on J: turbulent line-broadening in the quasi-static layers reduces the radiation force available to accelerate matter through the sonic point. If photospheric turbulence approaches sonic velocities, this mechanism reduces mass loss rates by factors > 3, which would partly account for the reduced rates found observationally for clumpy winds.Comment: Accepted by A&A; 9 pages, 4 figure

The radial velocity curve of HD153919 (4U1700-37) revisited

We have re-analysed all available high-resolution ultraviolet IUE spectra of the high-mass X-ray binary HD153919/4U1700-37. The radial velocity semi-amplitude of 20.6 +/- 1.0 km/s and orbital eccentricity of 0.22 +/- 0.04 agree very well with the values obtained earlier from optical spectra. They disagree with earlier conclusions for the same data reduced by Heap & Corcoran (1992) and by Stickland & Lloyd (1993).Comment: 6 pages, latex, figure included, Astronomy & Astrophysics, in pres

Candidate Coronagraphic Detections of Protoplanetary Disks around Four Young Stars

We present potential detections of H-band scattered light emission around four young star, selected from a total sample of 45 young stars observed with the CIAO coronagraph of the Subaru telescope. Two CTTS, CI Tau and DI Cep, and two WTTS, LkCa 14 and RXJ 0338.3+1020 were detected. In all four cases, the extended emission is within the area of the residual PSF halo, and is revealed only through careful data reduction. We compare the observed extended emission with simulations of the scattered light emission, to evaluate the plausibility and nature of the detected emission.Comment: 9 Figures, 40 page

Emission Line Galaxies in the STIS Parallel Survey I: Observations and Data Analysis

In the first three years of operation STIS obtained slitless spectra of approximately 2500 fields in parallel to prime HST observations as part of the STIS Parallel Survey (SPS). The archive contains almost 300 fields at high galactic latitude (|b|>30) with spectroscopic exposure times greater than 3000 seconds. This sample contains 220 fields (excluding special regions and requiring a consistent grating angle) observed between 6 June 1997 and 21 September 2000, with a total survey area of about 160 square arcminutes. At this depth, the SPS detects an average of one emission line galaxy per three fields. We present the analysis of these data, and the identification of 131 low to intermediate redshift galaxies detected by optical emission lines. The sample contains 78 objects with emission lines that we infer to be redshifted [OII]3727 emission at 0.43<z<1.7. The comoving number density of these objects is comparable to that of H-alpha emitting galaxies in the NICMOS parallel observations. One quasar and three probable Seyfert galaxies are detected. Many of the emission-line objects show morphologies suggestive of mergers or interactions. The reduced data are available upon request from the authors.Comment: 58 preprint pages, including 26 figures; accepted for publication in ApJ

Orbital and stochastic far-UV variability in the nova-like system V3885 Sgr

Highly time-resolved time-tagged FUSE satellite spectroscopic data are analysed to establish the far-ultraviolet (FUV) absorption line characteristics of the nova-like cataclysmic variable binary, V3885 Sgr. We determine the temporal behaviour of low (Ly_beta, CIII, NIII) and high (SIV, PV, OVI) ion species, and highlight corresponding orbital phase modulated changes in these lines. On average the absorption troughs are blueshifted due to a low velocity disc wind outflow. Very rapid (~ 5 min) fluctuations in the absorption lines are isolated, which are indicative of stochastic density changes. Doppler tomograms of the FUV lines are calculated which provide evidence for structures where a gas stream interacts with the accretion disc. We conclude that the line depth and velocity changes as a function of orbital phase are consistent with an asymmetry that has its origin in a line-emitting, localised disc-stream interaction region.Comment: Accepted for publication in MNRA

Complex nature of SNP genotype effects on gene expression in primary human leucocytes.

This is a freely-available open access publication. Please cite the published version which is available via the DOI link in this record.BACKGROUND: Genome wide association studies have been hugely successful in identifying disease risk variants, yet most variants do not lead to coding changes and how variants influence biological function is usually unknown. METHODS: We correlated gene expression and genetic variation in untouched primary leucocytes (n = 110) from individuals with celiac disease - a common condition with multiple risk variants identified. We compared our observations with an EBV-transformed HapMap B cell line dataset (n = 90), and performed a meta-analysis to increase power to detect non-tissue specific effects. RESULTS: In celiac peripheral blood, 2,315 SNP variants influenced gene expression at 765 different transcripts (< 250 kb from SNP, at FDR = 0.05, cis expression quantitative trait loci, eQTLs). 135 of the detected SNP-probe effects (reflecting 51 unique probes) were also detected in a HapMap B cell line published dataset, all with effects in the same allelic direction. Overall gene expression differences within the two datasets predominantly explain the limited overlap in observed cis-eQTLs. Celiac associated risk variants from two regions, containing genes IL18RAP and CCR3, showed significant cis genotype-expression correlations in the peripheral blood but not in the B cell line datasets. We identified 14 genes where a SNP affected the expression of different probes within the same gene, but in opposite allelic directions. By incorporating genetic variation in co-expression analyses, functional relationships between genes can be more significantly detected. CONCLUSION: In conclusion, the complex nature of genotypic effects in human populations makes the use of a relevant tissue, large datasets, and analysis of different exons essential to enable the identification of the function for many genetic risk variants in common diseases.Coeliac UKNetherlands Organization for Scientific ResearchCeliac Disease Consortium (an innovative cluster approved by the Netherlands Genomics Initiative and partly funded by the Dutch government)Netherlands Genomics InitiativeWellcome Trus

High-mass X-ray binaries and OB-runaway stars

High-mass X-ray binaries (HMXBs) represent an important phase in the evolution of massive binary systems. HMXBs provide unique diagnostics to test massive-star evolution, to probe the physics of radiation-driven winds, to study the process of mass accretion, and to measure fundamental parameters of compact objects. As a consequence of the supernova explosion that produced the neutron star (or black hole) in these systems, HMXBs have high space velocities and thus are runaways. Alternatively, OB-runaway stars can be ejected from a cluster through dynamical interactions. Observations obtained with the Hipparcos satellite indicate that both scenarios are at work. Only for a minority of the OB runaways (and HMXBs) a wind bow shock has been detected. This might be explained by the varying local conditions of the interstellar medium.Comment: 15 pages, latex (sty file included) with 5 embedded figures (one in jpg format), to appear in Proc. "Influence of binaries on stellar population studies", Eds. Vanbeveren, Van Rensberge