Evaluation of Hungarian Wines for Resveratrol by Overpressured Layer Chromatography

A method, including solid phase extraction sample preparation, overpressured layer chromatographic separation and subsequent densitometric evaluation, was developed for measurement of total resveratrol (cis- and trans-isomers) content of wine. The amount of resveratrol was determined in wine samples from different winemaking regions of Hungary. The total resveratrol was high in Hungarian red wines (3.6–11 mg/L), and much lower in white ones (0.04–1.5 mg/L)

CZE analysis of the antibiotic vancomycin in commercial formulations and in innovative microparticles

Vancomycin is a glicopeptide antibiotic, isolated from the soil microorganism Streptomyces orientalis, which is used for the treatment of severe infections caused by Gram-positive organisms; Vancomycin is administred in parenteral form for the treatment of endocarditis and other severe infections and in oral form for the treatment of colon infections. New formulations are in progress to obtain a controlled release of the drug in the colon site, in order to use the antibiotic for the treatment of specific intestinal patologies. The aim of this work is the development of a quick analytical method for the quality control of commercial oral capsules, vials for intravenous injections and innovative microparticles. The method is based on capillary zone electrophoresis. A 3D-CE Agilent instrument equipped with a diode-array detector was used; fused silica capillaries, with a total capillary length of 48.5 cm and an effective length of 8.5 cm (100 \ub5m internal diameter) were used. The BGE consisted of a 12.5 mM phosphate buffer, whose pH was adjusted to 2.5 using sodium hydroxide. The applied voltage was \u201320 kV, the detection wavelenght 210 nm, with the capillary thermostatted at 25\ub0C. The samples were diluted in water and injected by pressure (30 mbar for 3 s) from the anodic side of the capillary. An electrophoretic run is completed within 3.0 minutes, using Triprolidine as the internal standard. Good linearity was found in the Vancomycin concentration range from 500 ng/mL to 5 \ub5g/mL with satisfactory precision. After validation, the method was applied to the analysis of commercial capsules, vials and innovative microparticles; preliminary investigations gave satisfactory results

A versatile targeted metabolomics method for rapid quantification of multiple classes of phenolics in fruit and beverages

Compelling evidence of the health benefits of phenolic compounds and their impact on food quality have stimulated the development of analytical methods for the identification and quantification of these compounds in different matrices in recent years. We have developed a targeted metabolomics method for the quantification of 140 phenolics,such as benzoates, phenylpropanoids, coumarins, stilbenes, dihydrochalcones and flavonoids in fruit and tea extracts and wine using UPLC-MS/MS. Chromatography was optimised to achieve separation of the compounds over a period of 15 minutes and MRM transitions were selected for accurate quantification. The effectiveness of the method was validated by studying the detection and quantification limits, the linearity ranges of instrumental response and the repeatability of the analysis. The method was validated for the analysis of apples, berries, green tea and red wine and represents a valuable tool for food quality evaluation and breeding studies