FISTOLA BRONCO-BILIARE DA ECHINOCOCCOSI EPATICA E DIAFRAMMATICA

abstract

Rorc restrains the potency of ST2+ regulatory T cells in ameliorating intestinal graft-versus-host disease

Soluble stimulation-2 (ST2) is increased during graft-versus-host disease (GVHD), while Tregs that express ST2 prevent GVHD through unknown mechanisms. Transplantation of Foxp3- T cells and Tregs that were collected and sorted from different Foxp3 reporter mice indicated that in mice that developed GVHD, ST2+ Tregs were thymus derived and predominantly localized to the intestine. ST2-/- Treg transplantation was associated with reduced total intestinal Treg frequency and activation. ST2-/- versus WT intestinal Treg transcriptomes showed decreased Treg functional markers and, reciprocally, increased Rorc expression. Rorc-/- T cells transplantation enhanced the frequency and function of intestinal ST2+ Tregs and reduced GVHD through decreased gut-infiltrating soluble ST2-producing type 1 and increased IL-4/IL-10-producing type 2 T cells. Cotransfer of ST2+ Tregs sorted from Rorc-/- mice with WT CD25-depleted T cells decreased GVHD severity and mortality, increased intestinal ST2+KLRG1+ Tregs, and decreased type 1 T cells after transplantation, indicating an intrinsic mechanism. Ex vivo IL-33-stimulated Tregs (TregIL-33) expressed higher amphiregulin and displayed better immunosuppression, and adoptive transfer prevented GVHD better than control Tregs or TregIL-33 cultured with IL-23/IL-17. Amphiregulin blockade by neutralizing antibody in vivo abolished the protective effect of TregIL-33. Our data show that inverse expression of ST2 and RORγt in intestinal Tregs determines GVHD and that TregIL-33 has potential as a cellular therapy avenue for preventing GVHD

Differences in chemical, physical and microbiological characteristics of Italian burrata cheeses made in artisanal and industrial plants of Apulia Region

The burrata cheese is a traditional product from Southern Italy, consisting of an envelope of pasta filata (stretched curd) filled with cream and pasta filata strips (usually leftovers from mozzarella production). Physical [water activity (aw), pH], chemical (moisture, NaCl content) and microbiological [total viable count (TVC), Listeria monocytogenes, Salmonella spp., Yersinia enterocolitica, Bacillus cereus, Escherichia coli, Enterobacteriaceae, coagulase-positive staphylococci] characteristics of burrata cheeses manufactured in artisanal and industrial plants were evaluated. The artisanal burrata showed lower aw values in the filling and the final product. The same was recorded in the filling for the moisture, probably due to differences between the types of cream used in the artisanal and the industrial cheesemaking. The pH value of the filling differed between the two groups but no difference was recorded in the final product. Microbiological differences were also recorded, with higher values for TVC and E. coli in artisanal than industrial burrata. All samples were negative for the other microbialdeterminations, with the exception of coagulase-positive staphylococci and Y. enterocolitica, which were detected in artisanal burrata. Differences in cheesemaking process were probably responsible for the strong variability of the physical and chemical data between the two cheeses; furthermore, differences in the hygienic features were also recorded. Even though artisanal products showed lower aw and pH values and higher NaCl concentration, the higher E. coli loads highlighted the need for a more accurate compliance with hygienic procedures along the artisanal cheesemaking process

Welfare indicators during broiler slaughtering

1. The aim of this study was to identify the most relevant welfare indicators for unloading, lairage, stunning, killing and post-mortem inspection in a poultry slaughter plant. Different indicators were unloading duration, lairage time, environmental variables in the lairage facilities, shackling time and electrical variables used in the water bath. 2. Lairage time did not correlate strongly with dead on arrival. Heat stress was limited by means of ventilation systems, correct cage placement and appropriate stocking density per crate. The acceptable shackling period was about 30 s. 3. The presence of a corneal reflex showed that an animal was alive, while spontaneous wing flapping, spontaneous eye blinking and response to a painful stimulus were regarded as indicators of stunning efficiency. 4. It was concluded that the presence of recent traumatic injuries during the post-mortem inspection could be a valid means to establish whether corrective measures concerning the handling, transport and loading procedures should be taken

Manifesto on the Datafication of Mobility Across Borders

We present the Manifesto on the Datafication of Mobility Across Borders . Datafication is expanding the potential to produce and circulate information about people at unprecedented speed and scope. This is particularly revealed when people are “on the move” through territories of which they are not citizens. In this Manifesto, we are interested in the datafication practices and infrastructures that produce people as radical others. Practices of datafication and data infrastructures make people on the move kn owable, but they do not represent them neutrally. They often enact them as “alterity , ” as inherently alien others against whom an “us” can be identified. Allegedly implemented for security purposes, not always well designed, often sloppily applied, practices and infrastructures of datafication of people on the move as others run the risk o f subjecting vulnerable people to a perpetual state of precarity and securitization, and polities to long - term policies of expulsion. As sociologists of technology, ethnog raphers, political scholars, and software developers, we have witnessed with growing concern the recurrent instrumentalization of datafication for assessing identities of people on the move. The ten principles of this Manifesto are drawn from research cond ucted over seven years by the Processing Citizenship research team and discussed with the international scientific community involved in social studies of science and technology, migration, border and mobility studies, and security studies. We offer these principles based on best practices and empirical observation so that policymakers can hold to account national and European agencies tasked with home security functions, and IT developers can hold to account the infrastructures they design and implement

Hypo- and hyper-virulence in apricot trees infected by European stone fruit yellows

An apricot orchard, located in an area of north eastern Italy under serious pressure from European stone fruit yellows (ESFY) infection, has been monitored since the year it was planted (1990). During this time, most of the trees displayed symptoms or were shown by PCR analyses to be infected. Two groups of apricot trees were particularly interesting: some trees were asymptomatically infected while others recovered from the symptoms but not from the pathogen. In order to isolate those strains of the phytoplasma characterised by varying virulence, each of the two groups was used as mother plants and propagated. The new trees were used to constitute experimental orchards, where they were observed for the presence of symptoms and in part were tested by PCR, starting in 2003. The results obtained confirmed the presence of strains of the pathogen characterised by varying virulence. The strains originally present in infected apricot trees which recovered from the symptoms of ESFY were seen to be hypovirulent; none of the propagated infected trees ever showed symptoms of the disease. Surprisingly, the strains present in asymptomatic apricot mother plants were hypervirulent and the propagated trees always displayed severe symptoms. In the propagated trees, the transmission of the pathogen was higher in the hypervirulent strains than in the hypovirulent ones. A graft transmission trial carried out in the greenhouse using some of the identified hypo- and hypervirulent strains, confirmed the results obtained in open field. Real time PCR analyses showed that in the trees infected by hypovirulent strains the colonisation of the pathogen was lower than in those infected by the hypervirulent strains. It is possible to affirm that the hypovirulent strains were present in those mother plants which had originally recovered. The research will continue with the aim of verifying the possibility of cross protection among the identified hypo- and hypervirulent strains

Hypo- and hyper-virulence in apricot trees infected by European stone fruit yellows

An apricot orchard, located in an area of north eastern Italy under serious pressure from European stone fruit yellows (ESFY) infection, has been monitored since the year it was planted (1990). During this time, most of the trees displayed symptoms or were shown by PCR analyses to be infected. Two groups of apricot trees were particularly interesting: some trees were asymptomatically infected while others recovered from the symptoms but not from the pathogen. In order to isolate those strains of the phytoplasma characterised by varying virulence, each of the two groups was used as mother plants and propagated. The new trees were used to constitute experimental orchards, where they were observed for the presence of symptoms and in part were tested by PCR, starting in 2003. The results obtained confirmed the presence of strains of the pathogen characterised by varying virulence. The strains originally present in infected apricot trees which recovered from the symptoms of ESFY were seen to be hypovirulent; none of the propagated infected trees ever showed symptoms of the disease. Surprisingly, the strains present in asymptomatic apricot mother plants were hypervirulent and the propagated trees always displayed severe symptoms. In the propagated trees, the transmission of the pathogen was higher in the hypervirulent strains than in the hypovirulent ones. A graft transmission trial carried out in the greenhouse using some of the identified hypo- and hypervirulent strains, confirmed the results obtained in open field. Real time PCR analyses showed that in the trees infected by hypovirulent strains the colonisation of the pathogen was lower than in those infected by the hypervirulent strains. It is possible to affirm that the hypovirulent strains were present in those mother plants which had originally recovered. The research will continue with the aim of verifying the possibility of cross protection among the identified hypo- and hypervirulent strains.Keywords: ‘Candidatus Phytoplasma prunorum’, real-time PCR, Prunu

Eculizumab treatment: stochastic occurrence of C3 binding to individual PNH erythrocytes

C5 blockade by eculizumab prevents complement-mediated intravascular hemolysis in paroxysmal nocturnal hemoglobinuria (PNH). However, C3-bound PNH red blood cells (RBCs), arising in almost all treated patients, may undergo extravascular hemolysis reducing clinical benefits. Despite the uniform deficiency of CD55 and of CD59, there are always two distinct populations of PNH RBCs, with (C3+) and without (C3-) C3 binding

Influence of rainfall on E. coli concentrations in clams: results of collaboration between competent health authority and producers’ association in the Province of Fermo (Italy)

The Area Vasta di Fermo (the competent health authority of the Marche Region), in collaboration with the local Producers’ Association conducted a series of studies on the presence of E. coli as an indicator of faecal contamination in the environment, in shellfish and in fishery waters, and on the factors that can affect their presence. These studies, carried out from 2008 to 2011, included an assessment of the currents along the coast, of the precipitations, and data from the monitoring of E. coli on shellfish harvested in the collection areas were examined. The results showed that in most cases, small concentrations of microorganisms in shellfish corresponded to little or no precipitations, while an increase in the levels of E. coli was preceded by more or less abundant rainfalls. The conclusions suggest that it is advisable to carry out a more detailed risk analysis which should take into account the above-mentioned factors. Furthermore, monitoring alone based on the determination of indicator organisms, especially when carried out as a single analysis or with a small number of E. coli determinations, does not provide a satisfactory indication of safety. The regional surveillance plans should be applied timely and rigorously, together with on-site investigations aimed at identifying changes that can affect the presence of E. coli in shellfish. Food business operators themselves could implement good manufacturing practices to verify whether the microbiological parameters are within the prescribed limits after rainfalls, especially if heavy

Treatment of wounds colonized by multidrug resistant organisms in immune-compromised patients: a retrospective case series.

Immune-compromised patients incur a high risk of surgical wound dehiscence and colonization by multidrug resistant organisms. Common treatment has been debridement and spontaneous secondary healing.All immune-compromised patients referred to our Institution between March 1, 2010 and November 30, 2011 for dehiscent abdominal wounds growing multidrug resistant organisms were treated by serial wound debridements and negative pressure dressing. They were primarily closed, despite positive microbiological cultures, when clinical appearance was satisfactory.Nine patients were treated by direct wound closure, five had been treated previously by secondary intention healing.According to our results, fast healing can be safely obtained by closure of a clinically healthy wound, despite growth of multidrug resistant organisms, even in immune-compromised patients