Interaction between Yeast Cdc6 Protein and B-Type Cyclin/Cdc28 Kinases

During purification of recombinant Cdc6 expressed in yeast, we found that Cdc6 interacts with the critical cell cycle, cyclin-dependent protein kinase Cdc28. Cdc6 and Cdc28 can be coimmunoprecipitated from extracts, Cdc6 is retained on the Cdc28-binding matrix p13-agarose, and Cdc28 is retained on an affinity column charged with bacterially produced Cdc6. Cdc6, which is a phosphoprotein in vivo, contains five Cdc28 consensus sites and is a substrate of the Cdc28 kinase in vitro. Cdc6 also inhibits Cdc28 histone H1 kinase activity. Strikingly, Cdc6 interacts preferentially with B-type cyclin/Cdc28 complexes and not Cln/Cdc28 in log-phase cells. However, Cdc6 does not associate with Cdc28 when cells are blocked at the restrictive temperature in a cdc34 mutant, a point in the cell cycle when the B-type cyclin/Cdc28 inhibitor p40Sic1 accumulates and purified p40Sic1 inhibits the Cdc6/Cdc28 interaction. Deletion of the Cdc28 interaction domain from Cdc6 yields a protein that cannot support growth. However, when overproduced, the mutant protein can support growth. Furthermore, whereas overproduction of wild-type Cdc6 leads to growth inhibition and bud hyperpolarization, overproduction of the mutant protein supports growth at normal rates with normal morphology. Thus, the interaction may have a role in the essential function of Cdc6 in initiation and in restraining mitosis until replication is complete

Coupled KdV equations derived from atmospherical dynamics

Some types of coupled Korteweg de-Vries (KdV) equations are derived from an atmospheric dynamical system. In the derivation procedure, an unreasonable $y$-average trick (which is usually adopted in literature) is removed. The derived models are classified via Painlev\'e test. Three types of $\tau$-function solutions and multiple soliton solutions of the models are explicitly given by means of the exact solutions of the usual KdV equation. It is also interesting that for a non-Painlev\'e integrable coupled KdV system there may be multiple soliton solutions.Comment: 19 pages, 2 figure

Global axisymmetric stability analysis for a composite system of two gravitationally coupled scale-free discs

In a composite system of gravitationally coupled stellar and gaseous discs, we perform linear stability analysis for axisymmetric coplanar perturbations using the two-fluid formalism. The background stellar and gaseous discs are taken to be scale-free with all physical variables varying as powers of cylindrical radius $r$ with compatible exponents. The unstable modes set in as neutral modes or stationary perturbation configurations with angular frequency $\omega=0$.Comment: 7 pages using AAS styl

Buffalo Child Care Means Business: Full Study Report

[Excerpt] Buffalo Child Care Means Business presents the economic and business case for making Buffalo\u27s children the focus of economic development. The 2006 survey of 117 businesses located in downtown Buffalo, New York, documents the business sector\u27s present and projected reliance upon high quality child care services as a necessary component to optimum workplace recruitment, productivity and stability. This promising study highlights research specific to the Buffalo region measuring the cost the community bears as a result of low quality child care and early education. It draws upon nationally recognized economic development strategies to offer recommendations for a strategic child care plan integral to the City of Buffalo\u27s overall strategic initiatives to strengthen downtown\u27s attractiveness to successful enterprises. The early development needs of Buffalo\u27s children must be front and center if the potential economic power of broadly successful education is to be realized. With business, government, education and child care leaders at the table, Buffalo\u27s economic renaissance can be built on individual and social foundations that last a lifetime

Distribution of Spectral Lags in Gamma Ray Bursts

Using the data acquired in the Time To Spill (TTS) mode for long gamma-ray bursts (GRBs) collected by the Burst and Transient Source Experiment on board the Compton Gamma Ray Observatory (BATSE/CGRO), we have carefully measured spectral lags in time between the low (25-55 keV) and high (110-320 keV) energy bands of individual pulses contained in 64 multi-peak GRBs. We find that the temporal lead by higher-energy gamma-ray photons (i.e., positive lags) is the norm in this selected sample set of long GRBs. While relatively few in number, some pulses of several long GRBs do show negative lags. This distribution of spectral lags in long GRBs is in contrast to that in short GRBs. This apparent difference poses challenges and constraints on the physical mechanism(s) of producing long and short GRBs. The relation between the pulse peak count rates and the spectral lags is also examined. Observationally, there seems to be no clear evidence for systematic spectral lag-luminosity connection for pulses within a given long GRB.Comment: 20 pages, 4 figure

Determination of Wave Function Functionals: The Constrained-Search--Variational Method

In a recent paper [Phys. Rev. Lett. \textbf{93}, 130401 (2004)], we proposed the idea of expanding the space of variations in variational calculations of the energy by considering the approximate wave function $\psi$ to be a functional of functions $\chi: \psi = \psi[\chi]$ rather than a function. The space of variations is expanded because a search over the functions $\chi$ can in principle lead to the true wave function. As the space of such variations is large, we proposed the constrained-search-- variational method whereby a constrained search is first performed over all functions $\chi$ such that the wave function functional $\psi[\chi]$ satisfies a physical constraint such as normalization or the Fermi-Coulomb hole sum rule, or leads to the known value of an observable such as the diamagnetic susceptibility, nuclear magnetic constant or Fermi contact term. A rigorous upper bound to the energy is then obtained by application of the variational principle. A key attribute of the method is that the wave function functional is accurate throughout space, in contrast to the standard variational method for which the wave function is accurate only in those regions of space contributing principally to the energy. In this paper we generalize the equations of the method to the determination of arbitrary Hermitian single-particle operators as applied to two-electron atomic and ionic systems. The description is general and applicable to both ground and excited states. A discussion on excited states in conjunction with the theorem of Theophilou is provided.Comment: 26 pages, 4 figures, 5 table