Tomato EF-Tsmt, a functional mitochondrial translation elongation factor from higher plants

Ethylene-induced ripening in tomato (Lycopersicon esculentum) resulted in the accumulation of a transcript designated LeEF-Tsmt that encodes a protein with significant homology to bacterial Ts translational elongation factor (EF-Ts). Transient expression in tobacco and sunflower protoplasts of full-length and truncated LeEF-Tsmt- GFP fusion constructs and confocal microscopy observations clearly demonstrated the targeting of LeEF-Tsmt to mitochondria and not to chloroplasts and the requirement for a signal peptide for the proper sorting of the protein. Escherichia coli recombinant LeEF-Tsmt co-eluted from Ni-NTA resins with a protein corresponding to the molecular weight of the elongation factor EF-Tu of E. coli, indicating an interaction with bacterial EF-Tu. Increasing the GDP concentration in the extraction buffer reduced the amount of EF-Tu in the purified LeEF-Tsmt fraction. The purified LeEF-Tsmt stimulated the poly(U)-directed polymerization of phenylalanine 10-fold in the presence of EF-Tu. Furthermore, LeEF-Tsmt was capable of catalysing the nucleotide exchange reaction with E. coli EF-Tu. Altogether, these data demonstrate that LeEF-Tsmt encodes a functional mitochondrial EF-Ts. LeEFTsmt represents the first mitochondrial elongation factor to be isolated and functionally characterized in higher plants

The EU as a global rule of law promoter: the consistency and effectiveness challenges

This paper aims to examine the consistency and effectiveness of the EU as a global promoter of values by focusing on the rule of law, one of the key values on which the EU is based and which is also supposed to guide the EU’s external action. The paper first offers the diagnosis that the EU has failed to properly address a number of key issues: (i) what the EU seeks to promote under the heading ‘rule of law’; (ii) how it measures and monitors a country’s adherence to this principle and (iii) the disconnect between its external and internal policies and instruments. To address these issues, four key recommendations are made: (i) the adoption of a guidance note; (ii) the development of a transversal measurement and monitoring instrument; (iv) the adoption of a rule of law checklist and (iv) the revision of the role of EU Fundamental Rights Agency, with the view of transforming it into a ‘Copenhagen Commission’ with new powers and a broader geographical remit

The rule of law as a well‑established and well‑defined principle of EU law

Against increasing rule of law backsliding within the EU, the European Commission has presented the rule of law as a well-established and well-defined principle whose core meaning is furthermore shared as a common value among all Member States. In refute, the national governments of the two EU countries, which are both subject to special EU procedures on account of the systemic threat to the rule of law their repeated actions have caused, have claimed that the rule of law is neither defined in EU law, nor could it be defined in EU law. This article’s primary aim is to assess these conflicting assertions. It does so by first offering an overview of the EU legal framework on the basis of which it is shown that the rule of law, as asserted by the Commission, is a well-established constitutional principle of EU law. It furthermore shows that it is well-defined, not least because of the Court of Justice’s extensive case law, the European Commission’s definitional codification of it and most recently, the adoption of the Rule of Law Conditionality Regulation 2020/2092 which provides the first comprehensive allen compassing internal-oriented definition of the rule of law adopted by the EU co-legislators. This article furthermore contends that the EU’s understanding of the rule of law reflects what may be presented as a broad consensus in the European legal space on its core meaning and components; its legal use as a primary principle of judicial interpretation and a source from which standards of judicial review may be derived; and how the rule of law relates to other fundamental values. Finally, this article concludes by examining the reality of a potentially emerging East-West dissensus as regards the rule of law. In light of evidence of strong and widespread support for the rule of law in every single EU Member State in the face of top-down attempts to systemically undermine it, it is however submitted that there is no meaningful East-West divide but an authoritarian-liberal divide at elite level

The Gould's Belt Very Large Array Survey III. The Orion region

We present results from a high-sensitivity (60 $\mu$Jy), large-scale (2.26 square degree) survey obtained with the Karl G. Jansky Very Large Array as part of the Gould's Belt Survey program. We detected 374 and 354 sources at 4.5 and 7.5 GHz, respectively. Of these, 148 are associated with previously known Young Stellar Objects (YSOs). Another 86 sources previously unclassified at either optical or infrared wavelengths exhibit radio properties that are consistent with those of young stars. The overall properties of our sources at radio wavelengths such as their variability and radio to X-ray luminosity relation are consistent with previous results from the Gould's Belt Survey. Our detections provide target lists for followup VLBA radio observations to determine their distances as YSOs are located in regions of high nebulosity and extinction, making it difficult to measure optical parallaxes.Comment: Accepted for publication in ApJ; 51 pages, 15 figures, 5 table

Towards an automated analysis of video-microscopy images of fungal morphogenesis

7 páginas, 6 figuras.[EN] Fungal morphogenesis is an exciting field of cell biology and several mathematical models have been developed to describe it. These models require experimental evidences to be corroborated and, therefore, there is a continuous search for new microscopy and image analysis techniques. In this work, we have used a Canny-edge-detector based technique to automate the generation of hyphal profiles and calculation of morphogenetic parameters such as diameter, elongation rates and hyphoid fitness. The results show that the data obtained with this technique are similar to published data generated with manualbased tracing techniques and that have been carried out on the same species or genus. Thus, we show that application of edge detector-based technique to hyphal growth represents an efficient and accurate method to study hyphal morphogenesis. This represents the first step towards an automated analysis of videomicroscopy images of fungal morphogenesis.[ES] La morfogénesis de los hongos es un área de estudio de gran relevancia en la biología celular y en la que se han desarrollado varios modelos matemáticos. Los modelos matemáticos de procesos biológicos precisan de pruebas experimentales que apoyen y corroboren las predicciones teóricas y, por este motivo, existe una búsqueda continua de nuevas técnicas de microscopía y análisis de imágenes para su aplicación en el estudio del crecimiento celular. En este trabajo hemos utilizado una técnica basada en un detector de contornos llamado “Canny-edge-detector” con el objetivo de automatizar la generación de perfiles de hifas y el cálculo de parámetros morfogenéticos, tales como: el diámetro, la velocidad de elongación y el ajuste con el perfil hifoide, es decir, el perfil teórico de las hifas de los hongos. Los resultados obtenidos son similares a los datos publicados a partir de técnicas manuales de trazado de contornos, generados en la misma especie y género. De esta manera demostramos que la aplicación de esta técnica para el trazado de perfiles en hifas en crecimiento es un método eficaz y preciso para el estudio de la morfogénesis de hifas. Este trabajo representa el primer paso en la automatización de análisis de imágenes de video-microscopía de morfogénesis de hifas.This work was supported in part by the project Flora Micológica Ibérica V (REN2002-04068-C02-01GLO), Ministerio de Ciencia y Tecnología, Spain. Dr. Diéguez-Uribeondo was supported by a NATO postdoctoral fellowship.Peer reviewe

Capgras syndrome as a psychiatric manifestation in Parkinson’s disease: a case report and literature review

We present the case of a 58-year-old male patient who had symptoms of anxiety after witnessing a case of social violence in his community in 2005. After that, he presented symptoms of Parkinson Disease and in 2006 we established this as the main diagnosis. In 2009 he presented neuropsychiatric symptoms such as apathy, anhedonia, social isolation, blunted affect, visual and auditory hallucinations, paranoid delusions, soliloquies, and the false belief that his wife and daughter had been replaced by identical impostors. We established the diagnosis of Capgras Syndrome. This case is clinically relevant because of the presentation of its symptoms, its evolution and its presenting comorbidity

A. Court of Justice. Judicial independence under threat: the Court of Justice to the rescue

The ECJ’s ruling in Associação Sindical dos Juízes Portugueses is nothing short of ground-breaking. First, it is arguably the most important judgment since Les Verts as regards the meaning and scope of the principle of the rule of law in the EU legal system. Second, it comes close to being the EU equivalent of the US Supreme Court case of Gitlow as regards the principle of effective judicial protection

Rapid angular expansion of the ionized core of CRL 618

Context. During the transition from the asymptotic giant branch (AGB) to the planetary nebulae phase the circumstellar envelopes of most low- and intermediate-mass stars experience a dramatic change in morphology. CRL 618 exhibits characteristics of both an AGB and post-AGB star. It also displays a spectacular array of bipolar lobes with a dense equatorial region, which makes it an excellent object for studying the development of asymmetries in evolved stars. In recent decades, an elliptical compact HII region located in the center of the nebula has been seen to be increasing in size and flux. This seems to be due to the ionization of the circumstellar envelope by the central star, and it probably indicates the beginning of the planetary nebula phase for CRL 618. Aims. We aim to determine the physical conditions under which the ionization of the circumstellar envelope of CRL 618 began to take place as well as the subsequent propagation of the ionization front. Methods. We analyzed interferometric radio continuum data at ~5 and 22 GHz from observations carried out at seven epochs with the VLA. We traced the flux increase of the ionized region over a period of ~26 years. We measured the dimensions of the HII region directly from the brightness distribution images to determine the increase of its size over time. For one of the epochs we analyzed observations at six frequencies from which we estimated the electron density distribution. We carried out model calculations of the spectral energy distribution at two different epochs to corroborate our observational results. Results. We found that the radio continuum flux and the size of the ionized region have been increasing monotonically in the past three decades. The size of the major axis of the HII region shows a dependance on frequency, which has been interpreted as a result of the gradient of the electron density in this direction. The growth of the HII region is due to the expansion of an ionized wind whose mass-loss rate increased continuously for a period of ~100 years until a few decades ago, when the mass-loss rate experienced a sudden decline. Our results indicate that the circumstellar envelope began to be ionized around 1971, which marks the start of the planetary nebula phase of CRL 618. © ESO, 2013

Better late than never? On then European Commission's Rule of Law Framework and its first activation

This article first offers an overview of the European Commission's Rule of Law Framework, which was adopted in March 2014. The mechanism's potential effectiveness and the Commission's reasoning to justify its first activation against Poland in January 2016, when it has failed to do so against Hungary, are subsequently analyzed. While the Commission should be commended for seeking to address increasing rule of law backsliding at Member State level, our main submission is that reliance on the Rule of Law Framework alone, if only because of its soft and discursive nature, will not remedy a situation where systemic violations of EU values form part of a governmental plan to set up an 'illiberal' regime