Survey of the Endogonaceae in Minnesota With Synoptic Keys to Genera and Species

Sixteen species in the Endogonaceae (Zygomycotina) were identified from 22 different plant species from a native prairie, an intensively cultivated vegetable field, a reclaimed iron ore tailings basin, an undisturbed site adjacent to the iron ore tailings basin, and from a Pinus resinosa plantation. Seven species of Endogonaceae identified in this study are new records for Minnesota. Synoptic keys to genera and species are presented

A novel 3-hydroxypropionic acid-inducible promoter regulated by the LysR-type transcriptional activator protein MmsR of Pseudomonas denitrificans

MmsR (33.3 kDa) is a putative LysR-type transcriptional activator of Pseudomonas denitrificans. With the help of 3-hydroxypropionic acid (3-HP), an important platform chemical, MmsR positively regulates the expression of mmsA, which encodes methylmalonylsemialdehyde dehydrogenase, the enzyme involved in valine degradation. In the present study, the cellular function of MmsR and its binding to the regulatory DNA sequence of mmsA expression were investigated both in vivo and in vitro. Transcription of the mmsA was enhanced >140-fold in the presence of 3-HP. In the MmsR-responsive promoter region, two operators showing dyad symmetry, designated O-1 and O-2 and centered at the -79 and -28 positions, respectively, were present upstream of the mmsA transcription start site. An electrophoretic mobility shift assay indicated that MmsR binds to both operator sites for transcription activation, probably in cooperative manner. When either O-1 or O-2 or both regions were mutated, the inducibility by the MmsR-3-HP complex was significantly reduced or completely removed, indicating that both sites are required for transcription activation. A 3-HP sensor was developed by connecting the activation of MmsR to a green fluorescent readout. A more than 50-fold induction by 25 mM 3-HP was observed

A fund raiser\u27s guide for gaining access to corporate givers

This project identified, through both primary and secondary research, techniques for local United Way organizations to effectively gain access to key executives at mid-size corporations. The secondary research found that the trend in corporate giving is to demand much more recognition for philanthropy. Many corporations are combining charitable donations with their marketing efforts to target present or future customers. Primary research was conducted using mail questionnaires, which were sent to fund raisers and corporate givers, listing possible techniques for making contact with corporate givers. A list of recommended techniques for fund raisers to use was then compiled. Recommendations for fund raisers include: 1) make networking a priority; 2) highlight that the said agency helps local people; 3) use mutual colleagues to schedule appointments with new potential contributors, but do not expect the colleagues to sell said organization; 4) when going through the mail, enclose references and ample evidence of success; 5) avoid using phone calls and unscheduled visits as an initial means of contact; and 6) begin initial meetings by clearly demonstrating that said organization is not a large, bureaucratic organization. Fund raisers, who are responsible for raising money for non-profit organizations, may use these recommendations to gain access to potential contributors

Disease control in the flower garden

20 pages; includes photographs. This archival publication may not reflect current scientific knowledge or recommendations. Current information available from the University of Minnesota Extension: https://www.extension.umn.edu.Pfleger, F. L.. (1976). Disease control in the flower garden. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/168990

Evaluation of Two Commercially Available DNA Tests for Detection of Human Papillomavirus

Objective: This study was designed to compare the sensitivity, specificity, efficiency, positive and negative predictive values, and ease of use for 2 commercially available hybridization kits for detecting human papillomavirus (HPV) DNA: Oncor Southern blot (SB) (Oncor, Inc., Gaithersburg, MD) and Digene ViraType dot blot (DB) (Digene Diagnostics, Inc., Silver Spring, MD)

Trajectories of Experience Through the Pandemic: A Qualitative Longitudinal Dataset

Here, we present a dataset collected within a longitudinal interview study that has been conducted as part of a larger project (i.e., Viral Communication), exploring (changing) public attitudes and behaviours through the course of the pandemic in Germany. From a nationally representative survey, forty participants were purposively sampled on the basis of gender, age and socioeconomic status for the interviews. Each participant was interviewed three times within a 10 month time frame (between December 2020 and September 2021), with the exception of two dropouts from the study. The semi-structured interviews were developed to further elaborate on some of the responses in the survey instrument and to provide additional insights into topics and controversies surrounding the Covid-19 pandemic in Germany, such as information/misinformation, trust/distrust, compliance, vaccination, and conspiracy beliefs

Investigating practice integration of independent prescribing by community pharmacists using normalization process theory: a cross-sectional survey.

Independent prescribing (IP) has not been extensively investigated in community pharmacy (CP). Normalization Process Theory (NPT) constructs help explain how interventions are integrated into practice and include: 'coherence' (understanding), 'cognitive participation' (what promotes engagement), 'collective action' (integration with existing systems), 'reflexive monitoring (evaluation). The aim of this study was to use NPT to investigate the integration of pharmacist IP in CP. NHS Scotland Pharmacy First Plus (PFP) is a community pharmacy IP service. Questionnaire items were developed using the NPT derived Normalisation MeAsure Development (NoMAD) tool for an online survey of all PFP IP pharmacists. Demographic data were analysed descriptively and scale scores (calculated from item scores for the 4 NPT constructs) were used for inferential analysis. There was a 73% (88/120) response rate. Greater than 90% 'strongly agreed' / 'agreed' to NoMAD items relating to most NPT constructs. However, responses to 'collective action' items were diverse with more participants answering 'neither agree nor disagree' or 'disagree'. A statistically significant difference in NPT construct scale scores with significant p-values (ranging from p<0.001 to p=0.033) was shown on all the NPT constructs for the variable 'On average, how often do you consult with patients under the PFP service?'. This theory-based work offers perspectives on IP integration within CP. Despite its geographic focus this work offers insights relevant to wider contexts on IP integration. It shows 'collective action' focused 'organisation' and 'group process' challenges with a need for further work on staff training, resource availability and utilisation, working relationships, communication and management