Stress and the ecology of urban experience: Migrant mental lives in central Shanghai

Responding to claims in urban studies and epidemiology that modern urban living negatively affects the mental health of the poor and newcomers to the city, this article offers a different account based on an ethnography of a neighborhood in central Shanghai, where precarious rural migrant lives unfold. Drawing on the concept of “ecologies of experience” to recognize the making of everyday sensibilities and affective tensions in urban dwelling, it focuses on subjectivity formed in habits of negotiating the urban environment, in coping with troubled thoughts and feelings posed by precariousness. The article considers ecologies of experience arising in distinct prosaic locations - a public library, a large bookstore, and a café – found to be important in the everyday spatial practices of migrants, grounding to different degrees of success hopes for their present and future in the city. In such dwelling, the stresses to mental health – consistently described by migrants as “pressure” (yali) – seem to be moderated through varied forms of respite, slowing-down, and “moments of being,” though always in ambivalent ways. In recognizing the everyday materiality of urban living, the article looks beyond the tendency in studies of China's internal migration to read off migrant mental health outcomes from structural disadvantages related to work, welfare and living conditions. Conceptually, it opens new ground in thinking by acknowledging the role of the felt qualities of lived experience in managing mental states, building on work in geography, sociology and anthropology attentive to the affective resonances of place and to practices of urban negotiation.ESR

Dynamics of massive point vortices in binary mixture of Bose-Einstein condensates

We study the massive point-vortex model introduced in Ref. [Phys. Rev. A 101, 013630 (2020)], which describes two-dimensional point vortices of one species that have small cores of a different species. We derive the relevant Lagrangian itself, based on the time-dependent variational method with a two-component Gross-Pitaevskii (GP) trial function. The resulting Lagrangian resembles that of charged particles in a static electromagnetic field, where the canonical momentum includes an electromagnetic term. The simplest example is a single vortex with a rigid circular boundary, where a massless vortex can only precess uniformly. In contrast, the presence of a sufficiently large filled vortex core renders such precession unstable. A small core mass can also lead to small radial oscillations, which are, in turn, clear evidence of the associated inertial effect. Detailed numerical analysis of coupled two-component GP equations with a single vortex and small second-component core confirms the presence of such radial oscillations, implying that this more realistic GP vortex also acts as if it has a small massive core.Comment: 10 pages, 5 figure

Self-Organization, Layered Structure, and Aggregation Enhance Persistence of a Synthetic Biofilm Consortium

Microbial consortia constitute a majority of the earth’s biomass, but little is known about how these cooperating communities persist despite competition among community members. Theory suggests that non-random spatial structures contribute to the persistence of mixed communities; when particular structures form, they may provide associated community members with a growth advantage over unassociated members. If true, this has implications for the rise and persistence of multi-cellular organisms. However, this theory is difficult to study because we rarely observe initial instances of non-random physical structure in natural populations. Using two engineered strains of Escherichia coli that constitute a synthetic symbiotic microbial consortium, we fortuitously observed such spatial self-organization. This consortium forms a biofilm and, after several days, adopts a defined layered structure that is associated with two unexpected, measurable growth advantages. First, the consortium cannot successfully colonize a new, downstream environment until it selforganizes in the initial environment; in other words, the structure enhances the ability of the consortium to survive environmental disruptions. Second, when the layered structure forms in downstream environments the consortium accumulates significantly more biomass than it did in the initial environment; in other words, the structure enhances the global productivity of the consortium. We also observed that the layered structure only assembles in downstream environments that are colonized by aggregates from a previous, structured community. These results demonstrate roles for self-organization and aggregation in persistence of multi-cellular communities, and also illustrate a role for the techniques of synthetic biology in elucidating fundamental biological principles

Superfluid vortex dynamics in an elliptical boundary

Recent advances in cold atom platforms, providing experimental accessibility to real-time dynamics, have renewed interest in the motion of superfluid vortices in two-dimensional domains. Motivated by this development, we study the dynamics of a vortex in a two-dimensional incompressible superfluid inside an elliptical boundary. Employing the Joukowsky conformal map from a circle to an ellipse, we derive an analytical expression for the complex potential describing the hydrodynamic flow around the vortex. We integrate the resulting equations of motion, finding that the vortex moves along a nearly (but not exactly) elliptical trajectory. In addition, we obtain a simple closed expression for the vortex self-energy, which serves as the Hamiltonian of the system.Comment: 16 pages, 6 figure

Dynamics of a massive superfluid vortex in rk confining potentials

We study the motion of a superfluid vortex in condensates having different background density profiles, ranging from parabolic to uniform. The resulting effective point-vortex model for a generic power-law potential ¿rk can be experimentally realized with recent advances in optical-trapping techniques. Our analysis encompasses both empty-core and filled-core vortices. In the latter case, the vortex acquires a mass due to the presence of distinguishable atoms located in its core. The axisymmetry allows us to reduce the coupled dynamical equations of motion to a single radial equation with an effective potential Veff. In many cases, Veff has a single minimum, where the vortex precesses uniformly. The dynamics of the vortex and the localized massive core arises from the dependence of the energy on the radial position of the vortex and from the rk trap potential. We find that a positive vortex with small mass orbits in the positive direction, but the sense of precession can reverse as the core mass increases. Early experiments and theoretical studies on two-component vortices found some qualitatively similar behavior.Peer ReviewedPostprint (published version

The Role of Oxidation Compounds in Biofilm Growth on Polyethylene Geomembrane Barriers Used in Landfill

In a model study, polyethylene was preoxidized and incubated for a period of 7 months at 40°C in two different municipal solid waste leachates. During the postexperimental analyses, specific attention was paid to the carbonyl species and carboxylic acid depletion during the environmental exposure because it is well known that carboxylic acids are believed to be a potential substrate for the development of microorganisms. The results showed that the carbonyl as well as the carboxylic acid depletion observed follows first-order kinetics. The biofilm formation was characterized using a suite of analytical techniques, and its formation was compared with the carboxylic acid and carbonyl depletion profil

The Transcriptional Landscape of the Photosynthetic Model Cyanobacterium Synechocystis sp. PCC6803.

Cyanobacteria exhibit a great capacity to adapt to different environmental conditions through changes in gene expression. Although this plasticity has been extensively studied in the model cyanobacterium Synechocystis sp. PCC 6803, a detailed analysis of the coordinated transcriptional adaption across varying conditions is lacking. Here, we report a meta-analysis of 756 individual microarray measurements conducted in 37 independent studies-the most comprehensive study of the Synechocystis transcriptome to date. Using stringent statistical evaluation, we characterized the coordinated adaptation of Synechocystis' gene expression on systems level. Evaluation of the data revealed that the photosynthetic apparatus is subjected to greater changes in expression than other cellular components. Nevertheless, network analyses indicated a significant degree of transcriptional coordination of photosynthesis and various metabolic processes, and revealed the tight co-regulation of components of photosystems I, II and phycobilisomes. Detailed inspection of the integrated data led to the discovery a variety of regulatory patterns and novel putative photosynthetic genes. Intriguingly, global clustering analyses suggested contrasting transcriptional response of metabolic and regulatory genes stress to conditions. The integrated Synechocystis transcriptome can be accessed and interactively analyzed via the CyanoEXpress website (http://cyanoexpress.sysbiolab.eu)

Parental Expectations and Prosocial Behavior of Adolescents From Low-Income Backgrounds: A Cross-Cultural Comparison Between Three Countries¿Argentina, Colombia, and Spain

Parental expectations are influenced by cultural models, which in turn are subject to a great influence from historically fluctuating features of the socioeconomic background. Parental expectations seem to be linked to children¿s social and emotional development in terms of empathy and prosocial behavior. The current study aims to (a) compare low-income adolescents¿ perceptions of parental expectations of prosocial and antisocial behavior across three Latin countries (Argentina, Colombia, and Spain), (b) compare the empathy and prosocial behavior between the three countries, (c) compare the prosocial behavior between the three countries, and (d) study the effect of perceived parental expectations and empathy on the prosocial behavior of adolescents in all three of the countries studied in this research. The sample was made up of 446 Argentinean adolescents, 474 Colombian adolescents, and 632 Spanish adolescents. The Expected Parental Reactions Scale, Interpersonal Reactivity Index, and Prosocial Behavior Questionnaire were used to measure the variables included in this study. Results reveal considerable differences between children¿s perceptions of parental expectations in different countries. Results also show the existence of significant differences between male and female adolescents. In all three countries, girls score more highly than boys in prosocial behavior and empathy. Furthermore, we find that low-income Argentinean adolescents score more highly than Spanish and Colombian adolescents in prosocial behavior measures. Finally, expected parental reactions toward prosocial behavior and empathy seem to have an influence on the adolescents¿ development of prosocial behavior in all three countries

Advanced cell-based modeling of the royal disease: characterization of the mutated F9 mRNA

Essentials The Royal disease (RD) is a form of hemophilia B predicted to be caused by a splicing mutation. We generated an iPSC-based model of the disease allowing mechanistic studies at the RNA level. F9 mRNA analysis in iPSC-derived hepatocyte-like cells showed the predicted abnormal splicing. Mutated F9 mRNA level was very low but we also found traces of wild type transcripts. SUMMARY: Background The royal disease is a form of hemophilia B (HB) that affected many descendants of Queen Victoria in the 19th and 20th centuries. It was found to be caused by the mutation F9 c.278-3A>G. Objective To generate a physiological cell model of the disease and to study F9 expression at the RNA level. Methods Using fibroblasts from skin biopsies of a previously identified hemophilic patient bearing the F9 c.278-3A>G mutation and his mother, we generated induced pluripotent stem cells (iPSCs). Both the patient's and mother's iPSCs were differentiated into hepatocyte-like cells (HLCs) and their F9 mRNA was analyzed using next-generation sequencing (NGS). Results and Conclusion We demonstrated the previously predicted aberrant splicing of the F9 transcript as a result of an intronic nucleotide substitution leading to a frameshift and the generation of a premature termination codon (PTC). The F9 mRNA level in the patient's HLCs was significantly reduced compared with that of his mother, suggesting that mutated transcripts undergo nonsense-mediated decay (NMD), a cellular mechanism that degrades PTC-containing mRNAs. We also detected small proportions of correctly spliced transcripts in the patient's HLCs, which, combined with genetic variability in splicing and NMD machineries, could partially explain some clinical variability among affected members of the European royal families who had lifespans above the average. This work allowed the demonstration of the pathologic consequences of an intronic mutation in the F9 gene and represents the first bona fide cellular model of HB allowing the study of rare mutations at the RNA level