An LED-based Flasher System for VERITAS

We describe a flasher system designed for use in monitoring the gains of the photomultiplier tubes used in the VERITAS gamma-ray telescopes. This system uses blue light-emitting diodes (LEDs) so it can be operated at much higher rates than a traditional laser-based system. Calibration information can be obtained with better statistical precision with reduced loss of observing time. The LEDs are also much less expensive than a laser. The design features of the new system are presented, along with measurements made with a prototype mounted on one of the VERITAS telescopes.Comment: Accepted for publication in Nuclear Instruments and Methods in Physics Research

Radioelectric Field Features of Extensive Air Showers Observed with CODALEMA

Based on a new approach to the detection of radio transients associated with extensive air showers induced by ultra high energy cosmic rays, the experimental apparatus CODALEMA is in operation, measuring about 1 event per day corresponding to an energy threshold ~ 5. 10^16 eV. Its performance makes possible for the first time the study of radio-signal features on an event-by-event basis. The sampling of the magnitude of the electric field along a 600 meters axis is analyzed. It shows that the electric field lateral spread is around 250 m (FWHM). The possibility to determine with radio both arrival directions and shower core positions is discussed.Comment: Accepted for publication in Astroparticle Physic

Human Cytomegalovirus Infection Upregulates the Mitochondrial Transcription and Translation Machineries.

UNLABELLED: Infection with human cytomegalovirus (HCMV) profoundly affects cellular metabolism. Like in tumor cells, HCMV infection increases glycolysis, and glucose carbon is shifted from the mitochondrial tricarboxylic acid cycle to the biosynthesis of fatty acids. However, unlike in many tumor cells, where aerobic glycolysis is accompanied by suppression of mitochondrial oxidative phosphorylation, HCMV induces mitochondrial biogenesis and respiration. Here, we affinity purified mitochondria and used quantitative mass spectrometry to determine how the mitochondrial proteome changes upon HCMV infection. We found that the mitochondrial transcription and translation systems are induced early during the viral replication cycle. Specifically, proteins involved in biogenesis of the mitochondrial ribosome were highly upregulated by HCMV infection. Inhibition of mitochondrial translation with chloramphenicol or knockdown of HCMV-induced ribosome biogenesis factor MRM3 abolished the HCMV-mediated increase in mitochondrially encoded proteins and significantly impaired viral growth under bioenergetically restricting conditions. Our findings demonstrate how HCMV manipulates mitochondrial biogenesis to support its replication. IMPORTANCE: Human cytomegalovirus (HCMV), a betaherpesvirus, is a leading cause of morbidity and mortality during congenital infection and among immunosuppressed individuals. HCMV infection significantly changes cellular metabolism. Akin to tumor cells, in HCMV-infected cells, glycolysis is increased and glucose carbon is shifted from the tricarboxylic acid cycle to fatty acid biosynthesis. However, unlike in tumor cells, HCMV induces mitochondrial biogenesis even under aerobic glycolysis. Here, we have affinity purified mitochondria and used quantitative mass spectrometry to determine how the mitochondrial proteome changes upon HCMV infection. We find that the mitochondrial transcription and translation systems are induced early during the viral replication cycle. Specifically, proteins involved in biogenesis of the mitochondrial ribosome were highly upregulated by HCMV infection. Inhibition of mitochondrial translation with chloramphenicol or knockdown of HCMV-induced ribosome biogenesis factor MRM3 abolished the HCMV-mediated increase in mitochondrially encoded proteins and significantly impaired viral growth. Our findings demonstrate how HCMV manipulates mitochondrial biogenesis to support its replication.S.K. was supported by a European Molecular Biology Organization long-term fellowship (ALTF 887-2009). M.P.W is funded by a Wellcome Trust Senior Clinical Fellowship (108070/Z/15/Z). R.J.S. is supported by MRC grant (MR/L008734/1). P.J.L . is supported by a Wellcome Trust Principal Research Fellowship, grant (WT101835). J. S. is supported by MRC Programme grant (G0701279). J.R., L. V. and M.M. are supported by MRC as part of the core funding for the Mitochondrial Biology Unit (MC_U105697135). L.V. is also supported by EMBO (ALFT 701- 2013).This is the final version of the article. It first appeared from the American Society for Microbiology via http://dx.doi.org/10.1128/mBio.00029-1

Ultrasound-guided oblique approach for peripheral venous access in a phantom model

BACKGROUND: Ultrasound (US) vascular guidance is traditionally performed in transverse (T) and longitudinal (L) axes, each with drawbacks. We hypothesized that the introduction of a novel oblique (O) approach would improve the success of US-guided peripheral venous access. We examined emergency physician (EP) performance using the O approach in a gel US phantom. METHODS: In a prospective, case control study, EPs were enrolled from four levels of physician experience including postgraduate years one to three (PGY1, PGY2, PGY3) and attending physicians. After a brief training session, each participant attempted vessel aspiration using a linear probe in T, L, and O axes on a gel US phantom. Time to aspiration and number of attempts to aspiration were recorded. The approach order was randomized, and descriptive statistics were used. RESULTS: Twenty-four physicians participated. The first-attempt success rate was lower for O, 45.83%, versus 70.83% for T (p = 0.03) and 83.33% for L (p = 0.01). The average time to aspiration was 12.5 s (O) compared with 9.47 s (T) and 9.74 s (L), respectively. There were no significant differences between all four groups in regard to total amount of time and number of aspiration attempts; however, a trend appeared revealing that PGY3 and attending physicians tended to aspirate in less time and by fewer attempts in all three orientations when compared with the PGY2 and PGY1 physicians. CONCLUSION: In this pilot study, US-guided simulated peripheral venous access using a phantom gel model in a mixed user group showed that the novel oblique approach was not initially more successful versus T and L techniques

A classification of the X-ray and radio states of Cyg X-3 and their long-term correlations

We present a detailed classification of the X-ray states of Cyg X-3 based on the spectral shape and a new classification of the radio states based on the long-term correlated behaviour of the radio and soft X-ray light curves. We find a sequence of correlations, starting with a positive correlation between the radio and soft X-ray fluxes in the hard spectral state, changing to a negative one at the transition to soft spectral states. The temporal evolution can be in either direction on that sequence, unless the source goes into a very weak radio state, from which it can return only following a major radio flare. The flare decline is via relatively bright radio states, which results in a hysteresis loop on the flux-flux diagram. We also study the hard X-ray light curve, and find its overall anticorrelation with the soft X-rays. During major radio flares, the radio flux responds exponentially to the level of a hard X-ray high-energy tail. We also specify the detailed correspondence between the radio states and the X-ray spectral states. We compare our results to those of black-hole and neutron-star binaries. Except for the effect of strong absorption and the energy of the high-energy break in the hard state, the X-ray spectral states of Cyg X-3 closely correspond to the canonical X-ray states of black-hole binaries. Also, the radio/X-ray correlation closely corresponds to that found in black-hole binaries, but it significantly differs from that in neutron-star binaries. Overall, our results strongly support the presence of a black hole in Cyg X-3.Comment: MNRAS, in pres

Correlated variability of Mkn 421 at X-ray and TeV wavelengths on timescales of hours

Mkn 421 was observed for about two days with BeppoSAX, prior to and partly overlapping the start of a 1 week continuous exposure with ASCA in April 1998, as part of a world-wide multiwavelength campaign. A pronounced, well defined, flare observed in X-rays was also observed simultaneously at TeV energies by the Whipple Observatory's 10 m gamma-ray telescope. These data provide the first evidence that the X-ray and TeV intensities are well correlated on time-scales of hours.Comment: 4 pages, 1 figure, presented at the VERITAS Workshop on the TeV Astrophysics of Extragalactic Object

Complex Spectral Variability from Intensive Multi-wavelength Monitoring of Mrk421 in 1998

We conducted a multi-frequency campaign for the TeV blazar Mrk~421 in 1998 April. The campaign started from a pronounced high amplitude flare recorded by SAX and Whipple; ASCA observation started three days later. In the X-ray data, we detected multiple flares, occuring on time scales of about one day. ASCA data clearly reveal spectral variability. The comparison of the data from ASCA, EUVE and RXTE indicates that the variability amplitudes in the low energy synchrotron component are larger at higher photon energies. In TeV Gamma-rays, large intra-day variations -- which were correlated with the X-ray flux -- were observed when results from three Cherenkov telescopes are combined. The RMS variability of TeV Gamma--rays was similar to that observed in hard X-rays, above 10 keV. The X-ray light curve reveals flares which are almost symmetric for most of cases, implying the dominant time scale is the light crossing time through the emitting region. The structure function analysis based on the continuous X-ray light curve of seven days indicates that the characteristic time scale is ~0.5 day. The analysis of ASCA light curves in various energy bands appears to show both soft (positive) and hard (negative) lags. These may not be real, as systematic effects could also produce these lags, which are all much smaller than an orbit. If the lags of both signs are real, these imply that the particle acceleration and X-ray cooling time scales are similar.Comment: 10 pages, 4 figures, accepted for publication in ApJ Letter