Development of fad7-1 single mutant Arabidopsis thaliana plants that are resistant to aphids

Aphids are a group of sap-feeding insects that attack most of the world’s crops. The loss of function of fatty acid desaturase7 (FAD7) in Solanum lycopersicum (tomato plant) induces aphid resistance that is dependent upon the accumulation of plant defense hormones such as salicylic acid (SA). Tomato lacks most of the genetic resources found in the model plant Arabidopsis (Arabidopsis thaliana). There is an analogous fad7-1 line of Arabidopsis; however, the line has a background mutation, the glabra-1 (gl1), that causes the absence of trichomes (small hairs), which are essential to plant defense. In order to study aphid resistance, a single mutant line of fad7-1 mutants were developed using cross breeding between the fad7-1/gl1 mutant and wild-type plants. Homozygous fad7-1 mutants were then identified using the Kasajima DNA extraction method, followed by the use of single nucleotide polymorphism-polymerase chain reaction (SNP-PCR) primers using allele-specific PCR. A phenotypic screening was then performed to screen out the plants with the glabra-1 mutation using the presence or absence of trichomes. Two single Arabidopsis fad7-1 mutant lines were identified, and subsequently verified using a bioassay to be aphid resistant relative to other genotypes as seen in tomato

Use of Counter-balanced Smith Machine Affects Performance Measurements for Rebound Bench Press Throws

Rebound bench presses throws (RBT), often performed on a Smith machine, are used for assessment and training of upper body power. During a RBT, the stretch-shortening cycle potentiates performance in the concentric movement. Smith machines frequently utilize a counter-balance weight to reduce the net load on the barbell; however, the use of counter-balance weight affects measures of performance for RBT. PURPOSE: To evaluate how the use of a counter-balanced Smith machine affects performance measures for RBT. METHODS: Performance measures for the no counter-balanced (NCB) and counter-balanced (CB) RBT were assessed for 24 men (age: 23 ± 3 years, height: 179 ± 6 cm, mass: 91 ± 17 kg, bench press 1-repetition maximum [1RM]: 107 ± 18 kg). Each participant performed 2 sets of 2 repetitions of RBT for each condition at 30 % of their 1RM. Peak power, peak force, peak concentric and eccentric velocities, and duration of eccentric and concentric phases were measured using a linear accelerometer attached to the barbell; peak ground reaction force (GRF) was measured using a force plate. For each condition, data from the repetition with the highest peak power was used in further analyses. Peak EMG was measured for the right pectoral, deltoid and triceps muscles and normalized using peak EMG in the 1RM. RESULTS: Peak barbell measurements for power (NCB: 1220 ± 269 W, CB: 1069 ± 255 W), force (NCB: 906 ± 252 N, CB: 713 ± 143 N), and concentric (NCB: 2.54 ± 0.27 m•s-1, CB: 2.24 ± 0.32 m•s-1) and eccentric (NCB: -1.19 ± 0.46 m•s-1, CB: -0.95 ± 0.29 m•s-1) velocities were significantly (p\u3c0.05) higher for NCB compared to CB. The durations for the eccentric (NCB: 0.53 ± 0.16 s, CB: 0.64 ± 0.12 s) and concentric phases (NCB: 0.58 ± 0.58 s, CB: 0.77 ± 0.82 s), and peak pectoral EMG (NCB: 91 ± 21 % of 1RM, CB: 101 ± 24 % of 1RM) were lower for NCB compared to CB. Peak EMG for deltoid and triceps and peak GRF were unaffected by the use of counter-balance weights. CONCLUSION: The use of CB equipment resulted in reduced performance measurements (peak power, peak force, and peak eccentric and concentric velocities) for the RBT compared to NCB equipment. The lower peak eccentric stretch velocity likely resulted in a less effective stretch-shortening cycle for CB compared to NCB and thus helps explain the lower performance measurements found for CB

Violation of the Leggett-Garg inequality with weak measurements of photons

By weakly measuring the polarization of a photon between two strong polarization measurements, we experimentally investigate the correlation between the appearance of anomalous values in quantum weak measurements, and the violation of realism and non-intrusiveness of measurements. A quantitative formulation of the latter concept is expressed in terms of a Leggett-Garg inequality for the outcomes of subsequent measurements of an individual quantum system. We experimentally violate the Leggett-Garg inequality for several measurement strengths. Furthermore, we experimentally demonstrate that there is a one-to-one correlation between achieving strange weak values and violating the Leggett-Garg inequality.Comment: 5 pages, 4 figure

Likelihood-ratio ranking of gravitational-wave candidates in a non-Gaussian background

We describe a general approach to detection of transient gravitational-wave signals in the presence of non-Gaussian background noise. We prove that under quite general conditions, the ratio of the likelihood of observed data to contain a signal to the likelihood of it being a noise fluctuation provides optimal ranking for the candidate events found in an experiment. The likelihood-ratio ranking allows us to combine different kinds of data into a single analysis. We apply the general framework to the problem of unifying the results of independent experiments and the problem of accounting for non-Gaussian artifacts in the searches for gravitational waves from compact binary coalescence in LIGO data. We show analytically and confirm through simulations that in both cases the likelihood ratio statistic results in an improved analysis.Comment: 10 pages, 6 figure

Universal analytic properties of noise. Introducing the J-Matrix formalism

We propose a new method in the spectral analysis of noisy time-series data for damped oscillators. From the Jacobi three terms recursive relation for the denominators of the Pad\'e Approximations built on the well-known Z-transform of an infinite time-series, we build an Hilbert space operator, a J-Operator, where each bound state (inside the unit circle in the complex plane) is simply associated to one damped oscillator while the continuous spectrum of the J-Operator, which lies on the unit circle itself, is shown to represent the noise. Signal and noise are thus clearly separated in the complex plane. For a finite time series of length 2N, the J-operator is replaced by a finite order J-Matrix J_N, having N eigenvalues which are time reversal covariant. Different classes of input noise, such as blank (white and uniform), Gaussian and pink, are discussed in detail, the J-Matrix formalism allowing us to efficiently calculate hundreds of poles of the Z-transform. Evidence of a universal behaviour in the final statistical distribution of the associated poles and zeros of the Z-transform is shown. In particular the poles and zeros tend, when the length of the time series goes to infinity, to a uniform angular distribution on the unit circle. Therefore at finite order, the roots of unity in the complex plane appear to be noise attractors. We show that the Z-transform presents the exceptional feature of allowing lossless undersampling and how to make use of this property. A few basic examples are given to suggest the power of the proposed method.Comment: 14 pages, 8 figure

Challenging Perceptions of Disability through Performance Poetry Methods: The "Seen but Seldom Heard" Project.

This paper considers performance poetry as a method to explore lived experiences of disability. We discuss how poetic inquiry used within a participatory arts-based research framework can enable young people to collectively question society’s attitudes and actions towards disability. Poetry will be considered as a means to develop a more accessible and effective arena in which young people with direct experience of disability can be empowered to develop new skills that enable them to tell their own stories. Discussion of how this can challenge audiences to critically reflect upon their own perceptions of disability will also be developed

Synergistic Development, Test, and Qualification Approaches for the Ares I and V Launch Vehicles

The U.S. National Aeronautics and Space Administration (NASA) initiated plans to develop the Ares I and Ares V launch vehicles in 2005 to meet the mission objectives for future human exploration of space. Ares I is designed to provide the capability to deliver the Orion crew exploration vehicle (CEV) to low-Earth orbit (LEO), either for docking to the International Space Station (ISS) or docking with an Earth departure stage (EDS) and lunar lander for transit to the Moon. Ares V provides the heavy-lift capability to deliver the EDS and lunar lander to orbit. An integrated test plan was developed for Ares I that includes un-crewed flight validation testing and ground testing to qualify structural components and propulsion systems prior to operational deployment. The overall test program also includes a single development test flight conducted prior to the Ares I critical design review (CDR). Since the Ares V concept was formulated to maximize hardware commonality between the Ares V and Ares I launch vehicles, initial test planning for Ares V has considered the extensibility of test approaches and facilities from Ares I. The Ares V test plan was part of a successful mission concept review (MCR) in 2008

A burst search for gravitational waves from binary black holes

Compact binary coalescence (CBC) is one of the most promising sources of gravitational waves. These sources are usually searched for with matched filters which require accurate calculation of the GW waveforms and generation of large template banks. We present a complementary search technique based on algorithms used in un-modeled searches. Initially designed for detection of un-modeled bursts, which can span a very large set of waveform morphologies, the search algorithm presented here is constrained for targeted detection of the smaller subset of CBC signals. The constraint is based on the assumption of elliptical polarisation for signals received at the detector. We expect that the algorithm is sensitive to CBC signals in a wide range of masses, mass ratios, and spin parameters. In preparation for the analysis of data from the fifth LIGO-Virgo science run (S5), we performed preliminary studies of the algorithm on test data. We present the sensitivity of the search to different types of simulated CBC waveforms. Also, we discuss how to extend the results of the test run into a search over all of the current LIGO-Virgo data set.Comment: 12 pages, 4 figures, 2 tables, submitted for publication in CQG in the special issue for the conference proceedings of GWDAW13; corrected some typos, addressed some minor reviewer comments one section restructured and references updated and correcte

Weak measurement of photon polarization by back-action induced path interference

The essential feature of weak measurements on quantum systems is the reduction of measurement back-action to negligible levels. To observe the non-classical features of weak measurements, it is therefore more important to avoid additional back-action errors than it is to avoid errors in the actual measurement outcome. In this paper, it is shown how an optical weak measurement of diagonal (PM) polarization can be realized by path interference between the horizontal (H) and vertical (V) polarization components of the input beam. The measurement strength can then be controlled by rotating the H and V polarizations towards each other. This well-controlled operation effectively generates the back-action without additional decoherence, while the visibility of the interference between the two beams only limits the measurement resolution. As the experimental results confirm, we can obtain extremely high weak values, even at rather low visibilities. Our method therefore provides a realization of weak measurements that is extremely robust against experimental imperfections.Comment: 11 pages, 3 figure