Organization of aerobactin, hemolysin, and antibacterial resistance genes in lactose negative Escherichia coli strains of serotype O4 isolated from children with dhiarrea

Epidemiologically related, non-lactose-fermenting (NLF) Escherichia coli strains of serotype O4 have been isolated at a high frequency from children with diarrhea in Somalia (M. Nicoletti, F. Superti, C. Conti, A. Calconi, and C. Zagaglia, J. Clin. Microbiol. 26:524-529, 1988). In order to define the virulence potential of these strains, we characterized the replication properties of their high-molecular-weight plasmids and studied the genetic locations and organization of the aerobactin (aer) and hemolysin (hly) determinants encoded by 23 NLF O4 E. coli strains. Southern blot hybridizations, mobilization assays of nonconjugative plasmids, and incompatibility-exclusion experiments conducted with a conjugative incompatibility group FI (IncFI) plasmid showed that (i) 20 out of the 23 strains examined harbor a 160- to 180-kb IncFI plasmid that shares homology with the basic replicons RepFIA, RepFIB, and (except for the plasmid of one strain) RepFIC, and 22 strains also contain a 40- to 140-kb IncFII plasmid sharing homology with the RepFIIA replicon; (ii) the IncFI plasmid is nonconjugative and carries antibiotic resistance genes; (iii) the aer system is located on the IncFI plasmids and/or the chromosomes in the three strains not harboring IncFI, and it is found in an inverted orientation; (iv) the hly determinants are located on the chromosome, and their genetic organization is well conserved and closely resembles that of the reference hemolytic plasmid pHly152; and (v) Hly- mutants obtained by transposon insertion mutagenesis are not cytotoxic to HeLa cell monolayers, indicating that hemolysin is responsible for the high cytotoxic activity we have previously reported for these strains. The structural organization of the plasmid-encoded aer operon, together with the finding that those plasmids also carry antibiotic resistance genes, indicates that the IncFI plasmid of the NLF O4 E. coli strains studied more closely resembles aer-encoding virulence IncFI Salmonella R plasmids than E. coli ColV plasmids. The data presented here cannot rule out whether the strains examined are potentially intestinal or extraintestinal pathogens. Nevertheless, the genetic organization of the virulence genes, together with the epidemiological behavior and the wide spectrum of antibiotic resistance of the NLF O4 E. coli strains, indicates that these strains are structured as typical E. coli pathogenic isolates of human origin

Optimal Trajectories for Near-Earth-Objects Using Solar Electric Propulsion (SEP) and Gravity Assisted Maneuver

The future interplanetary missions will probably use the conventional chemical rockets to leave the sphere of influence of the Earth, and solar electric propulsion (SEP) to accomplish the other maneuvers of the mission. In this work the optimization of interplanetary missions using solar electric propulsion and Gravity Assisted Maneuver to reduce the costs of the mission, is considered. The high specific impulse of electric propulsion makes a Gravity Assisted Maneuver 1 year after departure convenient. Missions for several Near Earth Asteroids will be considered. The analysis suggests criteria for the definition of initial solutions demanded for the process of optimization of trajectories. Trajectories for the asteroid 2002TC70 are analyzed. Direct trajectories, trajectories with 1 gravity assisted from the Earth and with 2 gravity assisted from the Earth and either Mars are present. An indirect optimization method will be used in the simulations

Design Of Lunar-Gravity-Assisted Escape Trajectories

Lunar gravity assist is a means to boost the energy and C3 of an escape trajectory. Trajectories with two lunar gravity assists are considered and analyzed. Two approaches are applied and tested for the design of missions aimed at Near-Earth asteroids. In the first method, indirect optimization of the heliocentric leg is combined to an approximate analytical treatment of the geocentric phase for short escape trajectories. In the second method, the results of pre-computed maps of escape C3 are employed for the design of longer Sun-perturbed escape sequences combined with direct optimization of the heliocentric leg. Features are compared and suggestions about a combined use of the approaches are presented. The techniques are efficiently applied to the design of a mission to a near-Earth asteroid

Optimal low-thrust trajectories to asteroids through an algorithm based on differential dynamic programming

In this paper an optimisation algorithm based on Differential Dynamic Programming is applied to the design of rendezvous and fly-by trajectories to near Earth objects. Differential dynamic programming is a successive approximation technique that computes a feedback control law in correspondence of a fixed number of decision times. In this way the high dimensional problem characteristic of low-thrust optimisation is reduced into a series of small dimensional problems. The proposed method exploits the stage-wise approach to incorporate an adaptive refinement of the discretisation mesh within the optimisation process. A particular interpolation technique was used to preserve the feedback nature of the control law, thus improving robustness against some approximation errors introduced during the adaptation process. The algorithm implements global variations of the control law, which ensure a further increase in robustness. The results presented show how the proposed approach is capable of fully exploiting the multi-body dynamics of the problem; in fact, in one of the study cases, a fly-by of the Earth is scheduled, which was not included in the first guess solution

Has VZV epidemiology changed in Italy? Results of a seroprevalence study

The aim of the study was to evaluate if and how varicella prevalence has changed in Italy. In particular a seroprevalence study was performed, comparing it to similar surveys conducted in pre-immunization era. During 2013–2014, sera obtained from blood samples taken for diagnostic purposes or routine investigations were collected in collaboration with at least one laboratory/center for each region, following the approval of the Ethics Committee. Data were stratified by sex and age. All samples were processed in a national reference laboratory by an immunoassay with high sensitivity and specificity. Statutory notifications, national hospital discharge database and mortality data related to VZV infection were analyzed as well. A total of 3707 sera were collected and tested. In the studied period both incidence and hospitalization rates decreased and about 5 deaths per year have been registered. The seroprevalence decreased in the first year of life in subjects passively protected by their mother, followed by an increase in the following age classes. The overall antibody prevalence was 84%. The comparison with surveys conducted with the same methodology in 1996–1997 and 2003–2004 showed significant differences in age groups 1–19 y. The study confirms that in Italy VZV infection typically occurs in children. The impact of varicella on Italian population is changing. The comparison between studies performed in different periods shows a significant increase of seropositivity in age class 1–4 years, expression of vaccine interventions already adopted in some regions

Simple Δv approximation for optimization of debris-to-debris transfers

In this paper, a simple analytical method is developed to accurately approximate the transfer costs between two objects of the removal sequence. The accuracy of these estimations is verified by comparison with existing optimized solutions.Rendezvous transfers between two given orbits are dealt with using an accurate dynamic model that takes J2 perturbation into account. The state-of-the-art solution winner of the ninth Global Trajectory Optimization Competition (GTOC9) by the Jet Propulsion Laboratory (JPL) is used as benchmark to verify the accuracy of the method presented in this paper

Theoretical investigation of responsivity/NEP trade-off in NIR graphene/semiconductor schottky photodetectors operating at room temperature

In this work we theoretically investigate the responsivity/noise equivalent power (NEP) trade-off in graphene/semiconductor Schottky photodetectors (PDs) operating in the near-infrared regime and working at room temperature. Our analysis shows that the responsivity/NEP ratio is strongly dependent on the Schottky barrier height (SBH) of the junction, and we derive a closed analytical formula for maximizing it. In addition, we theoretically discuss how the SBH is related to the reverse voltage applied to the junction in order to show how these devices could be optimized in practice for different semiconductors. We found that graphene/n-silicon (Si) Schottky PDs could be optimized at 1550 nm, showing a responsivity and NEP of 133 mA/W and 500 fW/√ Hz, respectively, with a low reverse bias of only 0.66 V. Moreover, we show that graphene/n-germanium (Ge) Schottky PDs optimized in terms of responsivity/NEP ratio could be employed at 2000 nm with a responsivity and NEP of 233 mA/W and 31 pW/√ Hz, respectively. We believe that our insights are of great importance in the field of silicon photonics for the realization of Si-based PDs to be employed in power monitoring, lab-on-chip and environment monitoring applications

Osteopontin plasma levels and accelerated atherosclerosis in patients with CAD undergoing PCI: a prospective clinical study.

OBJECTIVES: Growing evidence supports the role played by inflammation in atherosclerosis. Identifying sensitive biomarkers is useful in predicting accelerated atherosclerosis. We investigated prospectively the relationship between plasma levels of inflammatory biomarkers [osteopontin, C-reactive protein (CRP), interleukin-6 (IL-6)] and instent restenosis, and rapid coronary plaque progression in patients with coronary artery disease (CAD) undergoing percutaneous coronary intervention (PCI). METHODS: We studied 77 patients with CAD: 45 affected by unstable angina/non-ST elevation myocardial infarction [acute coronary syndrome (ACS)], and 32 by chronic coronary syndrome (CCS). Plasma osteopontin, IL-6, and CRP levels were measured before intervention in all patients; measurements were carried out on the basis of the following time course at 1,15, 30, 90, and 180 days follow-up in a subgroup of 39 consenting patients. Clinical and biohumoral data were correlated with baseline and 6-month PCI follow-up angiography. RESULTS: Osteopontin, IL-6, and CRP were higher in patients with ACS than in those with CCS (analysis of variance: P<0.001, 0.05, and 0.05, respectively). Baseline osteopontin levels proved to be associated with rapid coronary plaque progression (P=0.005) and instent restenosis (P=0.05). The highest osteopontin levels were found in patients with CAD with both rapid plaque progression and instent restenosis (P=0.003). PCI increased inflammatory markers acutely, and osteopontin remained elevated in patients with ACS. Patients with ACS showed a higher percentage (74%) of rapid plaque progression than those with CCS (26%) (P<0.05). CONCLUSION: The study prospectively shows the link between inflammatory status and accelerated atherosclerosis in patients with CAD undergoing PCI. The baseline and persistent rise of osteopontin is an expression of its contribution to the accelerated plaque progression, and therefore osteopontin may be a useful prognostic biomarker

Robust Design Approaches for Hybrid Rocket Upper Stage

Computational costs of robust-based design optimization methods may be very high. Evaluation of new procedures for the management of uncertainty with applications to hybrid rocket engines is here carried out. Two newly developed procedures are presented (hybrid algorithm and iterated local search), and their performances are compared with those of two previously developed procedures (genetic algorithm and particle swarm optimization). A liquid oxygen/paraffin-based fuel hybrid rocket engine that powers the third stage of a Vega-like launcher is considered. The conditions at third-stage ignition are assigned, and a proper set of parameters are used to define the engine design and compute the payload mass. Uncertainties in the regression rate are taken into account. An indirect trajectory optimization approach is used to determine a mission-specific objective function, which takes into account both the payload mass and ability of the rocket to reach the required final orbit despite uncertainties. Results show that for this kind of problem, particle swarm optimization and iterated local search outperform the genetic algorithm, but the use of a local search operator may slightly improve its performance

Mars Sample Return Mission: Mars Ascent Vehicle Propulsion Design

The aim of this research is to analyze a potential Mars Sample Return (MSR) mission through the study of an optimized design of the Mars Ascent Vehicle (MAV) propulsion system. The main goal of the MSR mission is to return to Earth samples of rocks and dust collected by a rover operating on the surface of Mars, and conveyed to the MAV into an Orbit Sample (OS) canister. The MAV must accomplish an initial ascent phase from the Mars surface to a circular Low Mars Orbit (LMO) with a radius of 500 Km and 30° inclination, and then with its second stage it must circularize into the target LMO where it releases the OS payload. A combination of the MAV and a second vehicle, the Mars Earth Return Vehicle (MERV) orbiter, is required to fulfill the final return phase from Mars to the Earth. After completing three different phases of rendezvous operations, with a final Hohmann Transfer the MERV is able to bring the OS to Earth with its payload. A spreadsheet model enables the evaluation of two different MAV architecture: a two-stage solid rocket, and a two-stage hybrid rocket. The study is based on the main rocket science equations, including the Tsiolkovsky Rocket Equation that calculates the change in velocity Delta V for the two stages of the MAV and the amount of propellant needed for both stages. From the analysis it can be noted that the two-stage hybrid design has significant advantages, firstly in terms of Gross Lift Off Mass GLOM (270 Kg) when compared to the solid solution (355 Kg). The hybrid rocket also has lower mass by up to 60 Kg since it does not require a thermal igloo. Finally, the mass fractions for both stages are comparable, and the required Delta V for the hybrid stages are less than those needed for the solid, allowing considerable fuel savings. The hybrid solution is ultimately preferred, considering the best performance related to the thermal fuel properties enabling the MAV to safely operated in the harsh Martian environment