Metabolism of amino acid amides in Pseudomonas putida ATCC 12633

The metabolism of the natural amino acid L-valine, the unnatural amino acids D-valine, and D-, L-phenylglycine (D-, L-PG), and the unnatural amino acid amides D-, L-phenylglycine amide (D, L-PG-NH2) and L-valine amide (L-Val-NH2) was studied in Pseudomonas putida ATCC 12633. The organism possessed constitutive L-amidase activities towards L-PG-NH2 and L-Val-NH2, both following the same pattern of expression, suggesting the involvement of similarly regulated enzymes, or a common enzyme. Quite surprisingly, growth in mineral media with L-PG-NH2 resulted in variable, long lag phases of growth and strongly reduced L-amidase activities. Conversion of D-PG-NH2 into D-PG and L-PG also occurred and could be attributed to the presence of an inducible D-amidase and the racemization of the amino acid amide in combination with L-amidase activity, respectively. The further degradation of L-PG and D-PG involved constitutive L-PG aminotransferase and inducible D-PG dehydrogenase activities, respectively, both with a high degree of enantioselectivity. Amino acid racemase activity for D- and L-PG was not detected.

Study of Radiative Leptonic D Meson Decays

We study the radiative leptonic $D$ meson decays of D^+_{(s)}\to \l^+\nu_{\l}\gamma (\l=e,\mu,\tau), $D^0\to \nu\bar{\nu}\gamma$ and D^0\to \l^+\l^-\gamma ($l=e,\mu$) within the light front quark model. In the standard model, we find that the decay branching ratios of $D^+_{(s)}\to e^+\nu_e\gamma$, $D^+_{(s)}\to\mu^+\nu_{\mu}\gamma$ and $D^+_{(s)}\to\tau^+\nu_{\tau}\gamma$ are $6.9\times 10^{-6}$ ($7.7\times 10^{-5}$), $2.5\times 10^{-5}$ ($2.6\times 10^{-4}$), and $6.0\times 10^{-6}$ ($3.2\times 10^{-4}$), and that of D^0\to\l^+\l^-\gamma (\l=e,\mu) and $D^0\to\nu\bar{\nu}\gamma$ are $6.3\times 10^{-11}$ and $2.7\times 10^{-16}$, respectively.Comment: 23 pages, 6 Figures, LaTex file, a reference added, to be published in Mod. Phys. Lett.

A Note on Derivations of Lie Algebras

In this note, we will prove that a finite dimensional Lie algebra $L$ of characteristic zero, admitting an abelian algebra of derivations $D\leq Der(L)$ with the property $$L^n\subseteq \sum_{d\in D}d(L)$$ for some $n\geq 1$, is necessarily solvable. As a result, if $L$ has a derivation $d:L\to L$, such that $L^n\subseteq d(L)$, for some $n\geq 1$, then $L$ is solvable.Comment: 4 page