3.8-Micron Photometry During the Secondary Eclipse of the Extrasolar Planet HD 209458b

We report infrared photometry of the extrasolar planet HD 209458b during the time of secondary eclipse (planet passing behind the star). Observations were acquired during two secondary eclipses at the NASA Infrared Telescope Facility (IRTF) in September 2003. We used a circular variable filter (1.5-percent bandpass) centered at 3.8 microns to isolate the predicted flux peak of the planet at this wavelength. Residual telluric absorption and instrument variations were removed by offsetting the telescope to nearby bright comparison stars at a high temporal cadence. Our results give a secondary eclipse depth of 0.0013 +/- 0.0011, not yet sufficient precision to detect the eclipse, whose expected depth is approximately 0.002 - 0.003. We here elucidate the current observational limitations to this technique, and discuss the approach needed to achieve detections of hot Jupiter secondary eclipses at 3.8 microns from the ground.Comment: 5 pages, 5 figures, in press for MNRA

A Ground-Based Search for Thermal Emission from the Exoplanet TrES-1

Eclipsing planetary systems give us an important window on extrasolar planet atmospheres. By measuring the depth of the secondary eclipse, when the planet moves behind the star, we can estimate the strength of the thermal emission from the day side of the planet. Attaining a ground-based detection of one of these eclipses has proven to be a significant challenge, as time-dependent variations in instrument throughput and atmospheric seeing and absorption overwhelm the small signal of the eclipse at infrared wavelengths. We gathered a series of simultaneous L grism spectra of the transiting planet system TrES-1 and a nearby comparison star of comparable brightness, allowing us to correct for these effects in principle. Combining the data from two eclipses, we demonstrate a detection sensitivity of 0.15% in the eclipse depth relative to the stellar flux. This approaches the sensitivity required to detect the planetary emission, which theoretical models predict should lie between 0.05-0.1% of the stellar flux in our 2.9-4.3 micron bandpass. We explore the factors that ultimately limit the precision of this technique, and discuss potential avenues for future improvements.Comment: 10 pages, 1 table, four figures, accepted for publication in PAS

Rapid, quantitative determination of bacteria in water

A bioluminescent assay for ATP in water borne bacteria is made by adding nitric acid to a water sample with concentrated bacteria to rupture the bacterial cells. The sample is diluted with sterile, deionized water, then mixed with a luciferase-luciferin mixture and the resulting light output of the bioluminescent reaction is measured and correlated with bacteria present. A standard and a blank also are presented so that the light output can be correlated to bacteria in the sample and system noise can be substracted from the readings. A chemiluminescent assay for iron porphyrins in water borne bacteria is made by adding luminol reagent to a water sample with concentrated bacteria and measuring the resulting light output of the chemiluminescent reaction

Infrared Observations During the Secondary Eclipse of HD 209458 b II. Strong Limits on the Infrared Spectrum Near 2.2 Microns

We report observations of the transiting extrasolar planet, HD 209458 b, designed to detect the secondary eclipse. We employ the method of `occultation spectroscopy', which searches in combined light (star and planet) for the disappearance and reappearance of weak infrared spectral features due to the planet as it passes behind the star and reappears. Our observations cover two predicted secondary eclipse events, and we obtained 1036 individual spectra of the HD 209458 system using the SpeX instrument at the NASA IRTF in September 2001. Our spectra extend from 1.9 to 4.2 microns with a spectral resolution of 1500. We have searched for a continuum peak near 2.2 microns (caused by CO and water absorption bands), as predicted by some models of the planetary atmosphere to be approximately 6E-4 of the stellar flux, but no such peak is detected at a level of about 3E-4 of the stellar flux. Our results represent the strongest limits on the infrared spectrum of the planet to date and carry significant implications for understanding the planetary atmosphere. In particular, some models that assume the stellar irradiation is re-radiated entirely on the sub-stellar hemisphere predict a flux peak inconsistent with our observations. Several physical mechanisms can improve agreement with our observations, including the re-distribution of heat by global circulation, a nearly isothermal atmosphere, and/or the presence of a high cloud.Comment: Accepted to the Astrophysical Journal 17 pages, 6 figure

Determination of antimicrobial susceptibilities on infected urines without isolation

A method is described for the quick determination of the susceptibilities of various unidentified bacteria contained in an aqueous physiological fluid sample, particularly urine, to one or more antibiotics. A bacterial adenosine triphosphate (ATP) assay is carried out after the elimination of non-bacterial ATP to determine whether an infection exists. If an infection does exist, a portion of the sample is further processed, including subjecting parts of the portion to one or more antibiotics. Growth of the bacteria in the parts are determined, again by an ATP assay, to determine whether the unidentified bacteria in the sample are susceptible to the antibiotic or antibiotics under test

Thermal Phase Variations of WASP-12b: Defying Predictions

[Abridged] We report Warm Spitzer full-orbit phase observations of WASP-12b at 3.6 and 4.5 micron. We are able to measure the transit depths, eclipse depths, thermal and ellipsoidal phase variations at both wavelengths. The large amplitude phase variations, combined with the planet's previously-measured day-side spectral energy distribution, is indicative of non-zero Bond albedo and very poor day-night heat redistribution. The transit depths in the mid-infrared indicate that the atmospheric opacity is greater at 3.6 than at 4.5 micron, in disagreement with model predictions, irrespective of C/O ratio. The secondary eclipse depths are consistent with previous studies. We do not detect ellipsoidal variations at 3.6 micron, but our parameter uncertainties -estimated via prayer-bead Monte Carlo- keep this non-detection consistent with model predictions. At 4.5 micron, on the other hand, we detect ellipsoidal variations that are much stronger than predicted. If interpreted as a geometric effect due to the planet's elongated shape, these variations imply a 3:2 ratio for the planet's longest:shortest axes and a relatively bright day-night terminator. If we instead presume that the 4.5 micron ellipsoidal variations are due to uncorrected systematic noise and we fix the amplitude of the variations to zero, the best fit 4.5 micron transit depth becomes commensurate with the 3.6 micron depth, within the uncertainties. The relative transit depths are then consistent with a Solar composition and short scale height at the terminator. Assuming zero ellipsoidal variations also yields a much deeper 4.5 micron eclipse depth, consistent with a Solar composition and modest temperature inversion. We suggest future observations that could distinguish between these two scenarios.Comment: 19 pages, 10 figures, ApJ in press. Improved discussion of gravity brightenin

Non-detection of L-band Line Emission from the Exoplanet HD189733b

We attempt to confirm bright non-local thermodynamic equilibrium (non-LTE) emission from the exoplanet HD 189733b at 3.25 μm, as recently reported by Swain et al. based on observations at low spectral resolving power (λ/δλ ≈ 30). Non-LTE emission lines from gas in an exoplanet atmosphere will not be significantly broadened by collisions, so the measured emission intensity per resolution element must be substantially brighter when observed at high spectral resolving power. We observed the planet before, during, and after a secondary eclipse event at a resolving power λ/δλ = 27, 000 using the NIRSPEC spectrometer on the Keck II telescope. Our spectra cover a spectral window near the peak found by Swain et al., and we compare emission cases that could account for the magnitude and wavelength dependence of the Swain et al. result with our final spectral residuals. To model the expected line emission, we use a general non-equilibrium formulation to synthesize emission features from all plausible molecules that emit in this spectral region. In every case, we detect no line emission to a high degree of confidence. After considering possible explanations for the Swain et al. results and the disparity with our own data, we conclude that an astrophysical source for the putative non-LTE emission is unlikely. We note that the wavelength dependence of the signal seen by Swain et al. closely matches the 2ν_2 band of water vapor at 300 K, and we suggest that an imperfect correction for telluric water is the source of the feature claimed by Swain et al

Ability of γδ T cells to modulate the Foxp3 T cell response is dependent on adenosine.

Whether γδ T cells inhibit or enhance the Foxp3 T cell response depends upon their activation status. The critical enhancing effector in the supernatant is adenosine. Activated γδ T cells express adenosine receptors at high levels, which enables them to deprive Foxp3+ T cells of adenosine, and to inhibit their expansion. Meanwhile, cell-free supernatants of γδ T cell cultures enhance Foxp3 T cell expansion. Thus, inhibition and enhancement by γδ T cells of Foxp3 T cell response are a reflection of the balance between adenosine production and absorption by γδ T cells. Non-activated γδ T cells produce adenosine but bind little, and thus enhance the Foxp3 T cell response. Activated γδ T cells express high density of adenosine receptors and have a greatly increased ability to bind adenosine. Extracellular adenosine metabolism and expression of adenosine receptor A2ARs by γδ T cells played a major role in the outcome of γδ and Foxp3 T cell interactions. A better understanding of the functional conversion of γδ T cells could lead to γδ T cell-targeted immunotherapies for related diseases