The megalithic building of S.Erasmo di Cesi: architecture, astronomy, and landscape

Abstract. One of the most enigmatic megalithic buildings of Italy is the structure which lies on the S. Erasmo hill near Cesi, in Umbria, a huge complex encompassing an area of around 8000 square meters and enclosed by refined cyclopean walls. Although its date is uncertain, suggested dates comprise the Iron Age and archaic period, down to the third century B.C. The building’s function is also uncertain. Usually identified as a fortified structure, in fact there is a megalithic platform at the southern end of the enclosure which could have served as foundation of a temple or palace and, from the top of Monte Torre Maggiore, a complex of temples dating from the fourth century B.C. overlooks the hill. Similar combinations of megalithic buildings resting half-way to temples placed on high peaks are known to exist. In order to clarify the function of this structure and its position in relation to the surrounding landscape, with particular attention to its visibility and to the directions of visibility from the complex, as well as to the possible astronomical alignments, we present a multi-disciplinary approach to the study of the S. Erasmo complex, which includes the mapping of the sky at the various possible epochs of construction, the creation of a digital model of the landscape in forms of digital maps using Geographic Information System technologies, and a 3D model using various 3D software packages

Records of frugivorous fruit flies (Diptera: Tephritidae: Dacini) from the Comoro Archipelago

This paper summarizes current knowledge of the occurrence of Dacini fruit flies in the Comoro archipelago of the Indian Ocean. Ten species are confirmed as occurring there: Bactrocera invadens Drew, Tsuruta & White, 2005, Dacus bivittatus (Bigot, 1858), D. ciliatus Loew, 1862, D. etiennellus Munro, 1984, D. punctatifrons Karsch, 1887, D. vertebratus Bezzi, 1908 (all Dacina), Ceratitis capitata (Wiedemann, 1824), C. malgassa Munro, 1939, Neoceratitis cyanescens (Bezzi, 1923), and Trirhithrum nigerrimum (Bezzi, 1913) (all Ceratitidina). Records of Bactrocera Cucurbitae (Coquillett, 1899) remain unconfirmed. The fauna of the Comoros is briefly compared to that of other islands in the western Indian Ocean. (Résumé d'auteur

Host plant range of a fruit fly community (Diptera: Tephritidae): Does fruit composition influence larval performance?

Background: Phytophagous insects differ in their degree of specialisation on host plants, and range from strictly monophagous species that can develop on only one host plant to extremely polyphagous species that can develop on hundreds of plant species in many families. Nutritional compounds in host fruits affect several larval traits that may be related to adult fitness. In this study, we determined the relationship between fruit nutrient composition and the degree of host specialisation of seven of the eight tephritid species present in La Réunion; these species are known to have very different host ranges in natura. In the laboratory, larval survival, larval developmental time, and pupal weight were assessed on 22 fruit species occurring in La Réunion. In addition, data on fruit nutritional composition were obtained from existing databases. Results: For each tephritid, the three larval traits were significantly affected by fruit species and the effects of fruits on larval traits differed among tephritids. As expected, the polyphagous species Bactrocera zonata, Ceratitis catoirii, C. rosa, and C. capitata were able to survive on a larger range of fruits than the oligophagous species Zeugodacus cucurbitae, Dacus demmerezi, and Neoceratitis cyanescens. Pupal weight was positively correlated with larval survival and was negatively correlated with developmental time for polyphagous species. Canonical correspondence analysis of the relationship between fruit nutrient composition and tephritid survival showed that polyphagous species survived better than oligophagous ones in fruits containing higher concentrations of carbohydrate, fibre, and lipid. Conclusion: Nutrient composition of host fruit at least partly explains the suitability of host fruits for larvae. Completed with female preferences experiments these results will increase our understanding of factors affecting tephritid host range. (Résumé d'auteur

Immersive Appian Way health infrastructure: human centric Digital Twin (The PAAA Archeological Park of the Appian Way 12km State-Own Section, Unesco Candidature)

From Rome to Benevento, the Appian Way (Via Appia Antica) was born as a military road, 'Regina Viarum'. In 312 b.C., consul Appio Claudio extended the infrastructure for 132 miles to Capua. Many transformations and integration occurred across the centuries, resulting in a unique multi-stratified world heritage (landscape, architecture, archaeological remains and tombs along the military way). In the 19th century, Luigi Canina conceived the Appian Way as an outdoor museum, realizing a first state-own section along the 12km here surveyed and described. This year, the Ministry of Culture (MIC) has launched the UNESCO nomination for the road. The article discusses aspects of the mass digitization undertaken by the Parco Archeologico dell'Appia Antica (PAAA, the Archaeological Park of the Appian Way). The aim is to build a Digital Twin of the infrastructure supporting knowledge enhancement, preservation, design, communication and fruition. A virtual space where digital technologies and eXtended Reality are the digital arms of the contemporary Vitruvian humanistic mission and vision of the PAAA Appian Way as a source of wealth and healthiness for all the users and visitors

From “Learning by Doing” to Closing the Cycle with a Demonstration Effect: the Story about Nuclear Fuel Reprocessing in Argentina

Entre los emprendimientos que lanzó en la década del 60 la Comisión Nacional de Energía Atómica (CNEA), organismo argentino responsable de la investigación, el desarrollo y el asesoramiento en materia nuclear, se destaca el reprocesamiento de combustibles nucleares gastados. Dicha tecnología separa, con distintos fines, uranio, plutonio y residuos radiactivos del combustible irradiado. Este artículo aborda los proyectos de reprocesamiento que se encadenaron en CNEA entre 1962 y 1976: las Plantas de Reprocesamiento 1 y 2 y el Ensamble de Reprocesamiento Experimental. El foco está puesto en las decisiones técnicas y políticas que orientaron cada proyecto, sus continuidades y discontinuidades, así como los contextos que los habilitaron. La hipótesis que guía la argumentación es que los proyectos se corresponden con distintos paradigmas de política científica y tecnológica. Más precisamente, se corresponden con diferentes lógicas de definición de objetivos y de prácticas de trabajo, de justificación de sus contenidos, de estrategias de toma de decisión y de expectativas en torno a las posibilidades y los efectos del desarrollo local de ciencia y tecnología.Entre os empreendimentos lançados na década de 60 pela Comissão Nacional de Energia Atómica (CNEA) - organismo argentino responsável pela pesquisa, desenvolvimento e consultoria nuclear, destaca-se o reprocessamento de combustíveis nucleares gastos. Essa tecnologia separa, com diversos fins, urânio, plutônio e resíduos radiativos do combustível irradiado. Este artigo aborda os projetos de reprocessamento que se encadearam na CNEA entre 1962 e 1976: as Instalações de Reprocessamento 1 e 2, e a Montagem de Reprocessamento Experimental. O foco está nas decisões técnicas e nas políticas que orientaram cada projeto, suas continuidades e descontinuidades, bem como os contextos em que foram habilitados. A hipótese que orienta a argumentação é que os projetos se correspondem com diferentes paradigmas de política científica e tecnológica. Mais precisamente, eles se correspondem com diferentes lógicas de definição de objetivos e de práticas de trabalho, de justificação de seus conteúdos, de estratégias de tomada de decisão e de expectativas em torno às possibilidades e os efeitos do desenvolvimento local de ciência e tecnologia.Among the initiatives introduced in the 1960s by the National Atomic Energy Commission (CNEA, due to its initials in Spanish), the Argentine agency responsible for nuclear research, development and consultancy, the reprocessing of spent nuclear fuels stands out. This technology separates uranium, plutonium and radioactive waste from irradiated fuel for different uses. This paper addresses the CNEA fuel reprocessing projects that were launched between 1962 and 1976: Reprocessing Plants 1 and 2 and the Experimental Reprocessing Assembly. The focus is placed on the technical and political decisions that guided each project, their continuities and discontinuities, as well as the environments that enabled them. The hypothesis that guides the arguments made is that these projects correspond to different scientific-technological policy paradigms. More precisely, they correspond to different logics used to define objectives and work practices, rationales to justify their content, decision-making strategies and expectations regarding the possibilities and effects of the local development of this science and technology.Fil: Quilici, Domingo. Comisión Nacional de Energía Atómica; ArgentinaFil: Spivak L'Hoste, Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Sociales. Instituto de Desarrollo Económico y Social. Centro de Investigaciones Sociales; Argentin

A minimal cytomegalovirus intron A variant can improve transgene expression in different mammalian cell lines.

Made available in DSpace on 2018-07-01T01:26:14Z (GMT). No. of bitstreams: 1 Quilici2013ArticleAMinimalCytomegalovirusIntronA.pdf: 267916 bytes, checksum: a0678d07681cb4ac9d04a72f07b2d182 (MD5) Previous issue date: 2013-08-12bitstream/item/179295/1/Quilici2013-Article-AMinimalCytomegalovirusIntronA.pd

Sustained Activation of Lyn Tyrosine Kinase In Vivo Leads to Autoimmunity

Genetic ablation of the Lyn tyrosine kinase has revealed unique inhibitory roles in B lymphocyte signaling. We now report the consequences of sustained activation of Lyn in vivo using a targeted gain-of-function mutation (Lynup/up mice). Lynup/up mice have reduced numbers of conventional B lymphocytes, down-regulated surface immunoglobulin M and costimulatory molecules, and elevated numbers of B1a B cells. Lynup/up B cells are characterized by the constitutive phosphorylation of negative regulators of B cell antigen receptor (BCR) signaling including CD22, SHP-1, and SHIP-1, and display attributes of lymphocytes rendered tolerant by constitutive engagement of the antigen receptor. However, exaggerated positive signaling is also apparent as evidenced by the constitutive phosphorylation of Syk and phospholipase Cγ2 in resting Lynup/up B cells. Similarly, Lynup/up B cells show a heightened calcium flux in response to BCR stimulation. Surprisingly, Lynup/up mice develop circulating autoreactive antibodies and lethal autoimmune glomerulonephritis, suggesting that enhanced positive signaling eventually overrides constitutive negative signaling. These studies highlight the difficulty in maintaining tolerance in the face of chronic stimulation and emphasize the pivotal role of Lyn in B cell signaling

Proteomic network analysis of human uterine smooth muscle in pregnancy, labor, and preterm labor.

The molecular mechanisms involved in human uterine quiescence during gestation and the induction of labor at term or preterm are not completely known. Preterm delivery is associated with major morbidity and mortality and current efforts to prevent delivery until term are largely ineffective. Identification and semi-quantification of proteomic changes in uterine smooth muscle during pregnancy will allow for targeted research into how quiescence is maintained and what changes are associated with induction of labor. Examining preterm labor in this context will provide potential therapeutic targets for the management of preterm labor. We have recently performed two dimensional liquid chromatography coupled with tandem mass spectrometry on myometrial proteins isolated from pregnant patients in labor, pregnant patients not in labor, and pregnant patients in labor preterm. Using a conservative false discovery rate of 1% we have identified 2132 protein groups using this method and semi-quantitative spectral counting shows 201 proteins that have disparate levels of expression in preterm laboring samples. To our knowledge this is the first large scale proteomic study examining human uterine smooth muscle and this initial work has provided a target list for future experiments that can address how changing protein levels are involved in the induction of labor at term and preterm

Transcriptomic and metabolite analyses of Cabernet Sauvignon grape berry development

BACKGROUND: Grape berry development is a dynamic process that involves a complex series of molecular genetic and biochemical changes divided into three major phases. During initial berry growth (Phase I), berry size increases along a sigmoidal growth curve due to cell division and subsequent cell expansion, and organic acids (mainly malate and tartrate), tannins, and hydroxycinnamates accumulate to peak levels. The second major phase (Phase II) is defined as a lag phase in which cell expansion ceases and sugars begin to accumulate. Véraison (the onset of ripening) marks the beginning of the third major phase (Phase III) in which berries undergo a second period of sigmoidal growth due to additional mesocarp cell expansion, accumulation of anthocyanin pigments for berry color, accumulation of volatile compounds for aroma, softening, peak accumulation of sugars (mainly glucose and fructose), and a decline in organic acid accumulation. In order to understand the transcriptional network responsible for controlling berry development, mRNA expression profiling was conducted on berries of V. vinifera Cabernet Sauvignon using the Affymetrix GeneChip® Vitis oligonucleotide microarray ver. 1.0 spanning seven stages of berry development from small pea size berries (E-L stages 31 to 33 as defined by the modified E-L system), through véraison (E-L stages 34 and 35), to mature berries (E-L stages 36 and 38). Selected metabolites were profiled in parallel with mRNA expression profiling to understand the effect of transcriptional regulatory processes on specific metabolite production that ultimately influence the organoleptic properties of wine. RESULTS: Over the course of berry development whole fruit tissues were found to express an average of 74.5% of probes represented on the Vitis microarray, which has 14,470 Unigenes. Approximately 60% of the expressed transcripts were differentially expressed between at least two out of the seven stages of berry development (28% of transcripts, 4,151 Unigenes, had pronounced (≥2 fold) differences in mRNA expression) illustrating the dynamic nature of the developmental process. The subset of 4,151 Unigenes was split into twenty well-correlated expression profiles. Expression profile patterns included those with declining or increasing mRNA expression over the course of berry development as well as transient peak or trough patterns across various developmental stages as defined by the modified E-L system. These detailed surveys revealed the expression patterns for genes that play key functional roles in phytohormone biosynthesis and response, calcium sequestration, transport and signaling, cell wall metabolism mediating expansion, ripening, and softening, flavonoid metabolism and transport, organic and amino acid metabolism, hexose sugar and triose phosphate metabolism and transport, starch metabolism, photosynthesis, circadian cycles and pathogen resistance. In particular, mRNA expression patterns of transcription factors, abscisic acid (ABA) biosynthesis, and calcium signaling genes identified candidate factors likely to participate in the progression of key developmental events such as véraison and potential candidate genes associated with such processes as auxin partitioning within berry cells, aroma compound production, and pathway regulation and sequestration of flavonoid compounds. Finally, analysis of sugar metabolism gene expression patterns indicated the existence of an alternative pathway for glucose and triose phosphate production that is invoked from véraison to mature berries. CONCLUSION: These results reveal the first high-resolution picture of the transcriptome dynamics that occur during seven stages of grape berry development. This work also establishes an extensive catalog of gene expression patterns for future investigations aimed at the dissection of the transcriptional regulatory hierarchies that govern berry development in a widely grown cultivar of wine grape. More importantly, this analysis identified a set of previously unknown genes potentially involved in critical steps associated with fruit development that can now be subjected to functional testing.National Science Foundation Plant Genome Project (DBI-0217653); Bioinformatics program (DBI-0136561); National Institute of Health Biomedical Research Infrastructure Network (NIH-NCRR P20 RR16464; National Institute of Health IDeA Network of Biomedical Research Excellence (INBRE, RR-03-008); Nevada Agricultural Experimental Statio