Somatic cell nuclear transfer is associated with altered expression of angiogenic factor systems in bovine placentomes at term

Low efficiency of somatic cell cloning by nuclear transfer has been associated with alterations of placental vascular architecture. Placental growth and function depend on the growth of blood vessels; VEGF-A and bFGF are the most important factors controlling neovascularization and vascular permeability in the placenta. We hypothesize that the VEGF-A and bFGF systems are disrupted in placentomes from cloned animals, contributing to the placental abnormalities that are common in these clones. We determined mRNA expression and protein tissue localization of VEGF-A, bFGF, and their receptors in placentomes from cloned and non-cloned bovine fetuses at term. Real-time RT-PCR revealed that VEGFR-2 mRNA was increased in cloned male-derived placentomes, while mRNA of bFGF and its receptors were decreased in placentomes of cloned females. VEGF-A system proteins were found to be located in placentomal endothelial, maternal and fetal epithelial and stromal cells; there was a variable pattern of cellular distribution of these proteins in both cloned and non-cloned animals. Alterations in the expression of VEGF-A and bFGF systems suggest that angiogenic factors are involved in abnormal placental development in cloned gestations, contributing to impaired fetal development and poor survival ratesFAPESP 02/07392-7CAPES (PROBRAL grant 272/7)\ud CAPES (PROBRAL grant D/06/33937

Online fluorescence spectroscopy for the real-time evaluation of the microbial quality of drinking water

We assessed the utility of online fluorescence spectroscopy for the real-time evaluation of the microbial quality of untreated drinking water. Online fluorimeters were installed on the raw water intake at four groundwater -derived UK public water supplies alongside existing turbidity sensors that are used to forewarn of the presence of microbial contamination in the water industry. The fluorimeters targeted fluorescent dissolved organic matter (DOM) peaks at excitation/emission wavelengths of 280/365 nm (tryptophan-like fluorescence, TLF) and 280/450 nm (humic-like fluorescence, HLF). Discrete samples were collected for Escherichia coli, total bacterial cell counts by flow cytometry, and laboratory-based fluorescence and absorbance. Both TLF and HLF were strongly correlated with E. coli (r ¼ 0.71e0.77)and total bacterial cell concentrations (r ¼ 0.73e0.76), whereas the correlations between turbidity and E. coli (r ¼ 0.48) and total bacterial cell counts (r ¼ 0.40) were much weaker. No clear TLF peak was observed at the sites and all apparent TLF was considered to be optical bleed-through from the neighbouring HLF peak. Therefore, a HLF fluorimeter alone would be sufficient to evaluate the microbial water quality at these sources. Fluorescent DOM was also influenced by site operations such as pump start-up and the precipitation of cations on the sensor windows. Online fluorescent DOM sensors are a better indicator of the microbial quality of untreated drinking water than turbidity and they have wide-ranging potential applications within the water industry

Características morfológicas e estudo da vascularização do corpo lúteo cíclico de cabras ao longo do ciclo estral

Corpus luteum is a temporary endocrine gland that regulates either the estrous cycle and pregnancy. It presents extreme dependency on the adequate blood supply. This work aims to evaluate goat corpus luteum (CL) vascular density (VD) over the estrous cycle. For that purpose, 20 females were submitted to estrus synchronization/ovulation treatment using a medroxyprogesterone intra-vaginal sponge as well as intramuscular (IM) application of cloprostenol and equine chorionic gonadotrophine (eCG). After sponge removal, estrus was identified at about 72hs. Once treatment was over, female goats were then subdivided into 4 groups (n=5 each) and slaughtered on days 2, 12, 16 and 22 after ovulation (p.o). Ovaries were collected, withdrawn and weighted. CL and ovaries had size and area recorded. Blood samples were collected and the plasma progesterone (P4) was measured through RIA commercial kits. The VD was 24.42±6.66, 36.26±5.61, 8.59±2.2 and 3.97±1.12 vessels/mm² for days 2, 12, 16 and 22 p.o, respectively. Progesterone plasma concentrations were 0.49±0.08, 2.63±0.66, 0.61±0.14 and 0.22±0.04ng/ml for days 2, 12, 16 e 22 p.o, respectively. Studied parameters were affected by the estrous cycle phase. Values greater than 12 p.o were observed. In the present work we observed that ovulation occurred predominantly in the right ovary (70% of the animals), which in turn presented bigger measures than the contra lateral one. There is a meaningful relationship between the weight and size of the ovary and these of CL (r=0.87, r=0.70, respectively, p<0.05). It is possible to conclude that morphology of goat's ovaries and plasma progesterone concentration changed according to estrous cycle stages. We propose these parameters can be used as indicators of CL functional activity.O corpo lúteo é uma glândula endócrina temporária que regula tanto o ciclo estral quanto a prenhez, apresentando extrema dependência de aporte sanguíneo adequado. Objetivaram-se avaliar mudanças morfométricas dos ovários e densidade vascular (DV) dos corpos lúteos (CL) de cabras ao longo do ciclo estral (AOLC). Vinte animais foram submetidos ao tratamento para indução/sincronização do estro, usando esponjas intravaginais commedroxiprogesterona, associadas a aplicações intramusculares de cloprostenol e gonadotrofina coriônica eqüina. Após remoção das esponjas, o estro foi identificado em aproximadamente de 72h. Concluído o tratamento, as cabras foram subdivididas em 4 grupos (n=5 cada) para abate nos dias 2, 12, 16 e 22 após ovulação (p.o.). Posteriormente, foram retirados os ovários e realizadas as mensurações de peso, tamanho e área do órgão e dos CL. Amostras de sangue foram coletadas e a progesterona sérica (P4) mensurada utilizando-se RIA convencional. A DV média dos CL AOLC foi 24,42±6,66; 36,26±5,61; 8,59±2,2 e 3,97±1,12 vasos/mm2 para os dias 2, 12, 16 e 22 p.o., respectivamente. A concentração média de P4 foi de 0,49±0,08; 2,63±0,66; 0,61±0,14 e 0,22±0,04ng/ml para os dias 2, 12, 16 e 22 p.o., respectivamente. Os parâmetros em estudo também se mostraram afetados pela fase do ciclo estral, sendo observados os maiores (p < 0,05) valores no dia 12 p.o. Neste experimento, a ovulação ocorreu predominantemente no ovário direito (70% dos animais), o qual apresentou medidas maiores que o contralateral. Observou-se ainda alta correlação significativa entre o peso do ovário e o do CL (r=0,87; p<0,05) e entre o tamanho destes órgãos (r=0,70; p<0,05). Conclui-se que, a morfologia dos ovários de cabras e a concentração sérica de progesterona variam em função da fase do ciclo estral e podem ser utilizadas como parâmetro na avaliação funcional do órgão

Measurement of the cross section for isolated-photon plus jet production in pp collisions at √s=13 TeV using the ATLAS detector

The dynamics of isolated-photon production in association with a jet in proton–proton collisions at a centre-of-mass energy of 13 TeV are studied with the ATLAS detector at the LHC using a dataset with an integrated luminosity of 3.2 fb−1. Photons are required to have transverse energies above 125 GeV. Jets are identified using the anti- algorithm with radius parameter and required to have transverse momenta above 100 GeV. Measurements of isolated-photon plus jet cross sections are presented as functions of the leading-photon transverse energy, the leading-jet transverse momentum, the azimuthal angular separation between the photon and the jet, the photon–jet invariant mass and the scattering angle in the photon–jet centre-of-mass system. Tree-level plus parton-shower predictions from Sherpa and Pythia as well as next-to-leading-order QCD predictions from Jetphox and Sherpa are compared to the measurements

A search for resonances decaying into a Higgs boson and a new particle X in the XH → qqbb final state with the ATLAS detector

A search for heavy resonances decaying into a Higgs boson (H) and a new particle (X) is reported, utilizing 36.1 fb−1 of proton–proton collision data at collected during 2015 and 2016 with the ATLAS detector at the CERN Large Hadron Collider. The particle X is assumed to decay to a pair of light quarks, and the fully hadronic final state is analysed. The search considers the regime of high XH resonance masses, where the X and H bosons are both highly Lorentz-boosted and are each reconstructed using a single jet with large radius parameter. A two-dimensional phase space of XH mass versus X mass is scanned for evidence of a signal, over a range of XH resonance mass values between 1 TeV and 4 TeV, and for X particles with masses from 50 GeV to 1000 GeV. All search results are consistent with the expectations for the background due to Standard Model processes, and 95% CL upper limits are set, as a function of XH and X masses, on the production cross-section of the resonance

Full reduction of large finite random Ising systems by RSRG

We describe how to evaluate approximately various physical interesting quantities in random Ising systems by direct renormalization of a finite system. The renormalization procedure is used to reduce the number of degrees of freedom to a number that is small enough, enabling direct summing over the surviving spins. This procedure can be used to obtain averages of functions of the surviving spins. We show how to evaluate averages that involve spins that do not survive the renormalization procedure. We show, for the random field Ising model, how to obtain the "connected" 2-spin correlation function and the "disconnected" 2-spin correlation function. Consequently, we show how to obtain the average susceptibility and the average energy. For an Ising system with random bonds and random fields we show how to obtain the average specific heat. We conclude by presenting our numerical results for the average susceptibility and the "connected" 2-spin correlation function along one of the principal axes. (We believe this to be the first time, where the full three dimensional correlation is calculated and not just parameters like Nu or Eta.) The results for the average susceptibility are used to extract the critical temperature and critical exponents of the 3D random field Ising system.Comment: 30 pages, 17 figure

Propriedades físicas e químicas do solo em áreas com sistemas produtivos e mata na região da Zona da Mata mineira

Tendo em vista o potencial de produção e a extensão de área que ocupam os Latossolos, é de grande interesse estabelecer sistemas de manejo que não modifiquem o solo de forma a comprometer sua sustentabilidade. Nesse sentido, avaliaram-se as alterações em propriedades físicas e químicas de um Latossolo sob diferentes sistemas agrícolas na Zona da Mata mineira. Foram avaliadas áreas sob laranja, cana, pastagem e mata: as de laranja e pastagem têm mais de 20 anos de uso; a de cana é anterior (100 anos). A cana é remanescente de uso histórico por aproximadamente 120 anos. A amostragem foi realizada em trincheiras, nas profundidades de 0,0 a 0,2 e 0,2 a 0,4 m. Foram analisadas as características químicas: matéria orgânica do solo (MOS), pH em H2O, P disponível, Ca2+, Mg2+, K+, Al3+, H + Al, soma de bases (SB), CTC efetiva e total (t) e saturação por bases (V), e P-remanescente. As características físicas analisadas foram: textura, densidade de partículas, densidade do solo, porosidade total, curva de retenção de água, resistência do solo à penetração e estabilidade de agregados em água, bem como a relação capacidade de campo/porosidade total (CC/PT). Para análise dos dados, consideraram-se os usos agrícolas como tratamentos, em um delineamento inteiramente casualizado, na análise de variância de cada profundidade separadamente. Os sistemas agrícolas com laranjeira e canavial melhoraram a condição química do solo, aumentando os teores de nutrientes e diminuindo o Al3+ do complexo de troca, porém apresentaram redução dos teores de MOS e níveis intermediários de degradação física. O sistema com pastagem apresentou baixa fertilidade e caráter distrófico, redução acentuada do teor de MOS e degradação física mais avançada, evidenciada pelo aumento da densidade do solo e resistência à penetração, bem como redução da porosidade total e da estabilidade de agregados.Knowledge on chemical and physical soil properties provides the theoretical basis for a sustainable planning of natural resources. This study evaluated the alteration of chemical and physical soil characteristics in a Haplustox in the Zona da Mata region of the State of Minas Gerais (Brazil), under different crop systems. The systems evaluated were orange orchards, sugar-cane plantation, pasture and forest, as control. Orange orchards and pasture had been established more than 20 years before, both in succession to sugar-cane, previously grown for over a century. The sugar cane is a reminiscent of crop cultivated for more than 120 years. Soil was sampled in pits at depths of 0.0-0.2 and 0.2-0.4 m. The following chemical properties were evaluated: soil organic matter (MOS); pH in H2O, P, Ca2+, Mg2+, K, Al3+, H + Al, SB, CTC (t), and V, and the physical properties: texture, soil density, particle density, porosity, water retention curve, soil penetration resistance, and aggregate stability, as well as the ratio field capacity/soil total porosity. In the data evaluation, the crop systems represented treatments in a completely random design, and analysis of variance was performed for each depth separately. In the crop systems orange and sugar cane the chemical properties were improved, raising the nutrient levels and reducing the exchangeable Al3+, level but SOM was reduced and intermediate levels of physical degradation were observed. In the pasture system, low fertility and a dystrophic character were Stated, reduced SOM and high physical degradation, indicated by increased soil density and soil penetration resistance, and by lower porosity and aggregate stability