Characterization of an expressed Triticum monococcum Glu-A1y gene containing a premature termination codon in its C-terminal coding region

Premature termination codons (PTCs) are an important reason for the silence of highmolecular- weight glutenin subunits in Triticum species. Although the Glu-A1y gene is generally silent in common wheat, we here isolated an expressed Glu-A1y gene containing a PTC, named 1Ay8.3, from Triticum monococcum ssp. monococcum (AmAm, 2n = 2x = 14). Despite the presence of a PTC (TAG) at base pair positions 1879–1881 in the C-terminal coding region, this did not obviously affect 1Ay8.3 expression in seeds. This was demonstrated by the fact that when the PTC TAG of 1Ay8.3 was mutated to the CAG codon, the mutant in Escherichia coli bacterial cells expressed the same subunit as in the seeds. However, in E. coli, 1Ay8.3 containing the PTC expressed a truncated protein with faster electrophoretic mobility than that in seeds, suggesting that PTC translation termination suppression probably occurs in vivo (seeds) but not in vitro (E. coli). This may represent one of only a few reports on the PTC termination suppression phenomenon in genes

Effect of neutral hydrogen on edge impurity behavior in stochastic magnetic field layer of Large Helical Device

Two-dimensional (2-D) distribution of impurity line emissions has been measured with 2-D extreme ultraviolet (EUV) spectroscopy in Large Helical Device (LHD) for studying the edge impurity transport in stochastic magnetic field layer with three-dimensional (3-D) structure. The impurity behavior in the vicinity of two X-points at inboard and outboard sides of the toroidal plasma can be separately examined with the 2-D measurement. As a result, it is found that the carbon location changes from inboard to outboard X-points when the plasma axis is shifted from Rax = 3.6 m to 3.75 m. A 3-D simulation with EMC3-EIRENE code agrees with the result at Rax = 3.75 m but disagreed with the result at Rax = 3.60 m. The discrepancy between the measurement and simulation at Rax = 3.60 m is considerably reduced when an effect of neutral hydrogen localized in the inboard side is taken into account, which can modify the density gradient and friction force along the magnetic field

Effect of Boron on Magnetic Properties and Corrosion Resistance of High Energy Magnets

Element boron played an important role on magnetic properties and corrosion resistance of high energy density NdFeB products. The magnets were prepared by traditional powder metallurgy technique. It was found that with addition of 5.53 % B there was easy-plane phase formed. It owned not only lower remanence Br and coercivity iHc, but also poor corrosion resistance of magnet. As B content increase, the magnetic properties improved greatly, and obtained better properties at a content 5.7-5.87 % B. As increased B content further, superabundance B element formed too much B rich phase and led to Br decrease. Volume percent of phases calculated by theory formula, with the consumption of 0.1 % Nd oxided, it can be found that as B content increased from 5.53 %, the volume of main phase increased and easy-plane type Nd2Fe17 phase decreased in magnet, which resulted in Br, iHc increase. As B content increased higher than 5.82 %, more B rich phases formed which well isolated main phase grains and resulted in iHc increase. But too much B rich phase would decrease main phase proportion and lead to Br decrease. Examination of microstructure of magnets showed as B content increased from 5.53 % to 6.16 %, average matrix grain size was enlarged from 6.5µm to 8.2µm, and the proportion of sharp shape grains relatively increased as well. Abnormal grain growth was easily happened at higher B content. In addition, the decreased squareness Hk/iHc of magnets at higher B content also suggested inconsistent distribution of grain size and shape. Corrosion current density measured by polarization test for magnet with addition of 5.76 % B, near stoichiometric composition of Nd2Fe14B, owned lowest value and best corrosion resistance. Formation of Nd2Fe17 phase (lower B content) or B rich phase (higher B content) was not benefit for corrosion resistance improvement.</jats:p

Rhodamine derivative-modified filter papers for colorimetric and fluorescent detection of Hg2+ in aqueous media

10.1039/c2ta01072kJournal of Materials Chemistry A172526-253

Poly(dopamine acrylamide)-co-poly(propargyl acrylamide)-modified titanium surfaces for 'click' functionalization

10.1039/c2py00552bPolymer Chemistry34920-92