EU WATER POLICY: RESEARCH DEVELOPMENTS AND NEW MANAGEMENT TOOLS

At the end of 2000, the European Commission published its Water Framework Directive (WFD) in the Official Journal of the European Communities (2000/60/EC). This new legislation provides for achieving the sustainable management of water resources through its 26 articles that focus primarily on the improvement and protection of the quality of European water resources. The WFD adopts an integrated approach, based upon general principles deriving mainly from four disciplinary approaches, Geography; Ecology; Economics and Sociology. The new challenges posed to the people responsible for the management of water resources across the European Union include the "marrying" of existing national policies with the stipulations of the WFD. Research can support this transition by identifying compatibility and conflicts between legislative instruments, and by encouraging trans-national cooperative relationships. A relevant role of research is also foreseen in providing criteria and tools for conflict resolution by representing the goals of sustainable management in an objective and transparent way. The elaboration required for making planning decisions are increasing in number and complexity, requiring tools that help to organise and communicate the data that should be used to describe the decision context in terms of sustainability, in a holistic way by including environmental, economic and social information. These problems are being addressed by the MULINO Project, a 3-year research program aiming at producing a Decision Support System that will assist water managers in responding to the evolution of policies and management methodologies. The development of the system, which will be prepared in a software format, is being steered by a group of people from European water authorities. This steering committee is contributing to the policy analysis component of the research and to the software design which aims to be applicable in five different national contexts. This paper addresses the challenges and innovations that have been encountered in the second phase of research in which the first prototype of the software has been developed to operate in specific decision situations in each of MULINO's six case studies.Sustainable water management, EU policy, DSS tool, Resource /Energy Economics and Policy,

Managing the Impact of Climate Change on Migration: Evidence from Mexico

This paper uses state-level migration ow data between Mexico and the U.S. from 1999 to 2011 to investigate the migration response to climate shocks and the mitigating impact of an agricultural cash-transfer program (PROCAMPO) and a disaster fund (Fonden). Our results suggest that lower than average precipitations increase undocumented migration, especially from the most agricultural states. Fonden amounts are found to mitigate the effect of climate shocks on migration by lowering the undocumented migration response to precipitation anomalies. Similarly an increase in the state-level share of PROCAMPO funds to non-irrigated plots in the ejido sector decreases migration after a shock

Baseline anti-NS4a antibodies in combination with on-treatment quantitative HCV-RNA reliably identifies nonresponders to pegylated interferon-ribavirin combination therapy after 4 weeks of treatment

Background Early detection of nonresponders to hepatitis C therapy limits unnecessary exposure to treatment and its side-effects. A recent algorithm combining baseline anti-NS4a antibodies and on-treatment quantitative PCR identified nonresponders to a combination of interferon and ribavirin after 1 week of treatment. Aim To validate a stopping rule based on baseline anti-NS4a antibody levels and early on-treatment virological response in treatment-naive genotype 1 chronic hepatitis C patients treated with the current standard pegylated interferon and ribavirin combination therapy. Methods Eighty-nine genotype 1 patients from the Dynamically Individualized Treatment of hepatitis C Infection and Correlates of Viral/Host dynamics Study treated for 48 weeks with standard 180 mu g pegylated interferon (PEG-IFN)-alpha-2a (weekly) and ribavirin 1000-1200mg (daily) were analysed. Baseline anti-NS4a antibody enzyme-linked immunosorbent assay (NS4a AA 1687-1718) was performed on pretreatment serum. Hepatitis C virus-RNA was assessed at days 0, 1, 4, 7, 8, 15, 22, 29, weeks 6, 7, 8, 10, 12 and 6 weekly thereafter until end of treatment. Multiple regression logistic analysis was performed. Results Overall 54 of 89 (61%) patients achieved sustained virological response. A baseline anti-NS4a antibody titre less than 1/1250 correlated with absence of favourable initial viral decline according to variable response types (P=0.015). The optimal algorithm was developed using the combination of the absence of anti-NS4a Ab (= 100.000 IU/ml at week 4. This algorithm has a specificity of 43% and negative predictive value of 100% to detect nonresponse to standard PEG-IFN-alpha-2a and ribavirin therapy at fourth week of therapy (intention-to-treat analysis). Conclusion The decision to stop the therapy in genotype 1 chronic hepatitis C patients treated with PEG-IFN-alpha-2a and ribavirin can be confidently made after 4 weeks of treatment based on the absence of baseline anti-NS4a Ab and a week-4 hepatitis C virus-RNA above 100.000 IU/ml. Eur J Gastroenterol Hepatol 22:1443-1448 (C) 2010 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins

Autophagy gene haploinsufficiency drives chromosome instability, increases migration, and promotes early ovarian tumors.

Autophagy, particularly with BECN1, has paradoxically been highlighted as tumor promoting in Ras-driven cancers, but potentially tumor suppressing in breast and ovarian cancers. However, studying the specific role of BECN1 at the genetic level is complicated due to its genomic proximity to BRCA1 on both human (chromosome 17) and murine (chromosome 11) genomes. In human breast and ovarian cancers, the monoallelic deletion of these genes is often co-occurring. To investigate the potential tumor suppressor roles of two of the most commonly deleted autophagy genes in ovarian cancer, BECN1 and MAP1LC3B were knocked-down in atypical (BECN1+/+ and MAP1LC3B+/+) ovarian cancer cells. Ultra-performance liquid chromatography mass-spectrometry metabolomics revealed reduced levels of acetyl-CoA which corresponded with elevated levels of glycerophospholipids and sphingolipids. Migration rates of ovarian cancer cells were increased upon autophagy gene knockdown. Genomic instability was increased, resulting in copy-number alteration patterns which mimicked high grade serous ovarian cancer. We further investigated the causal role of Becn1 haploinsufficiency for oncogenesis in a MISIIR SV40 large T antigen driven spontaneous ovarian cancer mouse model. Tumors were evident earlier among the Becn1+/- mice, and this correlated with an increase in copy-number alterations per chromosome in the Becn1+/- tumors. The results support monoallelic loss of BECN1 as permissive for tumor initiation and potentiating for genomic instability in ovarian cancer

Application of the arbitrary Eulerian Lagrangian finite element formulation to the thermomechanical simulation of casting processes, with focus on pipe shrinkage prediction

International audienceThe Arbitrary Lagrangian-Eulerian formulation (ALE) has become an indispensable component of finite element thermomechanical computations of casting processes. As it is an intermediate formulation between the Lagrangian formulation (material convected mesh) and the Eulerian one (fixed mesh), it allows the simultaneous computation of important phenomena: Deformation and stresses affecting solidified regions, yielding the computation of air gap evolution at part/mold interfaces. In such regions, the formulation is essentially Lagrangian. Thermosolutal convection flow in the non solidified regions; here the ALE formulation tends to a pure Eulerian one (stationary mesh). Free surface evolution at top of risers, leading to the prediction of pipe defects (macroshrinkage). In this case the ALE formulation allows the follow up of the free surface. After a brief reminder of the constitutive equations to be used in thermomechanical modeling of solidification, the mechanical equations are presented and their resolution in the context of FEM-ALE. We insist on the transport analysis, a key-point of ALE, and present a validation of the original scheme that is used here. Finally, we focus on the prediction of pipe shrinkage formation and show two industrial examples

Green Production of Anionic Surfactant Obtained from Pea Protein

A pea protein isolate was hydrolyzed by a double enzyme treatment method in order to obtain short peptide sequences used as raw materials to produce lipopeptides-based surfactants. Pea protein hydrolysates were prepared using the combination of Alcalase and Flavourzyme. The influence of the process variables was studied to optimize the proteolytic degradation to high degrees of hydrolysis. The average peptide chain lengths were obtained at 3–5 amino acid units after a hydrolysis of 30 min with the mixture of enzymes. Then, N-acylation in water, in presence of acid chloride (C12 and C16), carried out with a conversion rate of amine functions of 90%, allowed to obtain anionic surfactant mixtures (lipopeptides and sodium fatty acids). These two steps were performed in water, in continuous and did not generate any waste. This process was therefore in line with green chemistry principles. The surface activities (CMC, foaming and emulsifying properties) of these mixtures were also studied. These formulations obtained from natural renewable resources and the reactions done under environmental respect, could replace petrochemical based surfactants for some applications

Interdisciplinary assessment of sea-level rise and climate change impacts on the lower Nile delta, Egypt

Copyright © 2015 Elsevier. NOTICE: this is the author’s version of a work that was accepted for publication in Science of the Total Environment. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Science of the Total Environment Vol. 503-504 (2015), DOI: 10.1016/j.scitotenv.2014.06.111CLImate-induced changes on WAter and SECurity (CLIWASEC) was a cluster of three complementary EC-FP7 projects assessing climate-change impacts throughout the Mediterranean on: hydrological cycles (CLIMB - CLimate-Induced changes on the hydrology of Mediterranean Basins); water security (WASSERMed - Water Availability and Security in Southern EuRope and the Mediterranean) and human security connected with possible hydro-climatic conflicts (CLICO - CLImate change hydro-COnflicts and human security). The Nile delta case study was common between the projects. CLIWASEC created an integrated forum for modelling and monitoring to understand potential impacts across sectors. This paper summarises key results from an integrated assessment of potential challenges to water-related security issues, focusing on expected sea-level rise impacts by the middle of the century. We use this common focus to illustrate the added value of project clustering. CLIWASEC pursued multidisciplinary research by adopting a single research objective: sea-level rise related water security threats, resulting in a more holistic view of problems and potential solutions. In fragmenting research, policy-makers can fail to understand how multiple issues can materialize from one driver. By combining efforts, an integrated assessment of water security threats in the lower Nile is formulated, offering policy-makers a clearer picture of inter-related issues to society and environment. The main issues identified by each project (land subsidence, saline intrusion - CLIMB; water supply overexploitation, land loss - WASSERMed; employment and housing security - CLICO), are in fact related. Water overexploitation is exacerbating land subsidence and saline intrusion, impacting on employment and placing additional pressure on remaining agricultural land and the underdeveloped housing market. All these have wider implications for regional development. This richer understanding could be critical in making better policy decisions when attempting to mitigate climate and social change impacts. The CLIWASEC clustering offers an encouraging path for the new European Commission Horizon 2020 programme to follow.European Commission Seventh Framework Program CLICOEuropean Commission Seventh Framework Program CLIMBEuropean Commission Seventh Framework Program WASSERMe

WNT signaling regulates self-renewal and differentiation of prostate cancer cells with stem cell characteristics

Prostate cancer cells with stem cell characteristics were identified in human prostate cancer cell lines by their ability to form from single cells self-renewing prostaspheres in non-adherent cultures. Prostaspheres exhibited heterogeneous expression of proliferation, differentiation and stem cell-associated makers CD44, ABCG2 and CD133. Treatment with WNT inhibitors reduced both prostasphere size and self-renewal. In contrast, addition of Wnt3a caused increased prostasphere size and self-renewal, which was associated with a significant increase in nuclear Β-catenin, keratin 18, CD133 and CD44 expression. As a high proportion of LNCaP and C4-2B cancer cells express androgen receptor we determined the effect of the androgen receptor antagonist bicalutamide. Androgen receptor inhibition reduced prostasphere size and expression of PSA, but did not inhibit prostasphere formation. These effects are consistent with the androgen-independent self-renewal of cells with stem cell characteristics and the androgen-dependent proliferation of transit amplifying cells. As the canonical WNT signaling effector Β-catenin can also associate with the androgen receptor, we propose a model for tumour propagation involving a balance between WNT and androgen receptor activity. That would affect the self-renewal of a cancer cell with stem cell characteristics and drive transit amplifying cell proliferation and differentiation. In conclusion, we provide evidence that WNT activity regulates the self-renewal of prostate cancer cells with stem cell characteristics independently of androgen receptor activity. Inhibition of WNT signaling therefore has the potential to reduce the self-renewal of prostate cancer cells with stem cell characteristics and improve the therapeutic outcome.Peer reviewe

Amorphous determination in calcium sulfoaluminate materials by external and internal methods

Sitio Web de la revista: http://www.icevirtuallibrary.com/toc/jadcr/currentExternal standard methodology has been successfully applied to quantify the known amounts of amorphous component that are increasingly added to cement-related materials. The consistency and accuracy of the methodology were demonstrated by the R2 values of the least-square fits determined against weighed amorphous amounts, which were close to 1.0 in all the series. This method requires common laboratory X-ray powder diffractometers (knowing the equipment constant) and avoids the dilution/alteration of the sample. However, the obtained values may be biased due to poor particle statistics. On the other hand, internal standard analysis in transmission geometry is suitable to determine the absolute amorphous and crystalline non-quantified contents that are less biased owing to enhanced particle averaging statistics. This method is, however, experimentally tedious due to the addition of the internal standard and the sample loading in the X-ray diffraction sample holders which represent inherent drawbacks to follow amorphous evolutions