Neonate Intestinal Immune Response to CpG Oligodeoxynucleotide Stimulation

Background: The development of mucosal vaccines is crucial to efficiently control infectious agents for which mucosae are the primary site of entry. Major drawbacks of these protective strategies are the lack of effective mucosal adjuvant. Synthetic oligodeoxynucleotides that contain several unmethylated cytosine-guanine dinucleotide (CpG-ODN) motifs are now recognized as promising adjuvants displaying mucosal adjuvant activity through direct activation of TLR9-expressing cells. However, little is known about the efficacy of these molecules in stimulating the intestinal immune system in neonates. Methodology/Principal Findings: First, newborn mice received CpG-ODN orally, and the intestinal cytokine and chemokine response was measured. We observed that oral administration of CpG-ODN induces CXC and CC chemokine responses and a cellular infiltration in the intestine of neonates as detected by immunohistochemistry. We next compared the efficiency of the oral route to intraperitoneal administration in stimulating the intestinal immune responses of both adults and neonates. Neonates were more responsive to TLR9-stimulation than adults whatever the CpG-ODN administration route. Their intestinal epithelial cells (IECs) indirectly responded to TLR9 stimulation and contributed to the CXC chemokine response, whereas other TLR9-bearing cells of the lamina-propria produced CC chemokines and Th1-type cytokines. Moreover, we showed that the intestine of adult exhibited a significantly higher level of IL10 at homeostasis than neonates, which might be responsible for the unresponsiveness to TLR9-stimulation, as confirmed by our findings in IL10-deficient mice. Conclusions/Significance: This is the first report that deciphers the role played by CpG-ODN in the intestine of neonates. This work clearly demonstrates that an intraperitoneal administration of CpG-ODN is more efficient in neonates than in adults to stimulate an intestinal chemokine response due to their lower IL-10 intestinal level. In addition we report the efficiency of the oral route at inducing intestinal chemokine responses in neonate that might be taken into consideration for further vaccine development against neonatal diseases

Terranimo® – ein webbasiertes Modell zur Abschätzung des Bodenverdichtungsrisikos

Um die Druckverhältnisse unter Landmaschinen zu simulieren und das Bodenverdichtungsrisiko einschätzen zu können, wurde das webbasierte Modell Terranimo® entwickelt. Darin kann mithilfe von verfügbaren Reifenkennwerten und in Abhängigkeit von der Oberbodenfestigkeit die Kontaktfläche und die Druckverteilung zwischen Reifen und Boden beschrieben werden. Mithilfe von Pedotransferfunktionen wird die Bodenfestigkeit (Vorbelastung) vom Tongehalt und der Saugspannung im Boden abgeleitet. Das Verdichtungsrisiko wird durch den Vergleich von Bodenfestigkeit und ausgeübtem Bodendruck abgeschätzt: Bleibt der durch die Landmaschine ausgeübte Bodendruck unterhalb der aktuellen Bodenfestigkeit, so können das Verdichtungsrisiko minimiert und negative Veränderungen der Bodenstruktur – und somit der Bodenfunktionen – vermieden werden.Based on experimental data from wheeling experiments, a web-based model for the simulation of stress and the evaluation of the soil compaction risk under agricultural machinery named Terranimo® has been developed. Terranimo® incorporates a model for prediction of contact area, shape and stress distribution in the tyre soil interface from wheel load and readily-available tyre parameters and the topsoil strength. In Terranimo® pedotransfer functions are used to estimate soil strength from clay content and matric potential. Principally, by limiting the imposed stress to below soil strength, the risk of soil compaction and undesirable changes of soil structure – and hence soil functions – can be minimized

An interdisciplinary approach towards improved understanding of soil deformation during compaction

International audienceSoil compaction not only reduces available pore volume in which fluids are stored, but it alters the arrangement of soil constituents and pore geometry, thereby adversely impacting fluid transport and a range of soil ecological functions. Quantitative understanding of stress transmission and deformation processes in arable soils remains limited. Yet such knowledge is essential for better predictions of effects of soil management practices such as agricultural field traffic on soil functioning. Concepts and theory used in agricultural soil mechanics (soil compaction and soil tillage) are often adopted from conventional soil mechanics (e.g. foundation engineering). However, in contrast with standard geotechnical applications, undesired stresses applied by agricultural tyres/tracks are highly dynamic and last for very short times. Moreover, arable soils are typically unsaturated and contain important secondary structures (e.g. aggregates), factors important for affecting their soil mechanical behaviour. Mechanical processes in porous media are not only of concern in soil mechanics, but also in other fields including geophysics and granular material science. Despite similarity of basic mechanical processes, theoretical frameworks often differ and reflect disciplinary focus. We review concepts from different but complementary fields concerned with porous media mechanics and highlight opportunities for synergistic advances in understanding deformation and compaction of arable soils. We highlight the important role of technological advances in non-destructive measurement methods at pore (X-ray tomography) and soil profile (seismic) scales that not only offer new insights into soil architecture and enable visualization of soil deformation, but are becoming instrumental in the development and validation of new soil compaction models. The integration of concepts underlying dynamic processes that modify soil pore spaces and bulk properties will improve the understanding of how soil management affect vital soil mechanical, hydraulic and ecological functions supporting plant growth

Soil assessment in Denmark:Towards soil functional mapping and beyond

Soil provides essential ecosystem services sustaining and improving human life, but mapping soil functions is an ongoing challenge. Denmark has a long history of carrying out soil assessments − originally in order to determine tax revenues for the king, and, more recently, for aiding policymakers and farmers. This knowledge has supported the development of intensive agricultural systems while maintaining the provision of ecosystem services (e.g., clean water). Getting an overview of historical soil surveys and pedological mapping approaches can generate useful information for mapping soil, identifying gaps and proposing directions for future research. In this review, we explore the evolution of soil and environmental inventories, the historical development of soil mapping methods, and how these factors contributed to a better spatial understanding of soil functions. Specifically, we discuss soil functions related to water regulation (e.g., drainage, groundwater and water surface interactions, water table), water filtering (e.g., nitrogen leaching), carbon sequestration (e.g., peatlands), agricultural production (e.g., land suitability, wheat yields), and threats related to soil degradation (e.g., soil erosion). Denmark has benefitted from a government-coordinated approach, promoting detailed and systematic national soil surveys and environmental monitoring programmes. The large databases produced in the surveys formed the basis for mapping several soil properties and functions at increasingly high resolutions over the last many years based on developments in machine learning. In contrast to methodological advances in soil mapping and relevant contributions to pedometric research, we identified a lack of spatial information on soil biodiversity. Detailed spatial information about soil functions is essential to address global issues, such as climate change, food security and water security, and the experience of mapping soil functions in Denmark can be a source of inspiration to other parts of the world.</p

The state of soils in Europe

This report delves into the intricate interplay between drivers, pressures and impacts on soil in the 32 Member States of the European Environment Agency (EEA), along with six cooperating countries from the West Balkans, Ukraine and UK, shedding light on the multifaceted challenges facing soil conservation efforts. Our analysis shows the complex interactions among various factors, both anthropogenic and natural, shaping soil degradation processes and their subsequent consequences. We highlight key findings, including the significant impacts of soil degradation on agriculture, ecosystem resilience, water quality, biodiversity, and human health, underscoring the urgent need for comprehensive soil management strategies. Moreover, our examination of citizen science initiatives underlines the importance of engaging the public in soil monitoring and conservation efforts. This work emphasises the policy relevance of promoting sustainable soil governance frameworks, supported by research, innovation, and robust soil monitoring schemes, to safeguard soil health and ensure the long-term resilience of ecosystems.JRC.D.3 - Land Resources and Supply Chain Assessment