Knee Kinematics Estimation Using Multi-Body Optimisation Embedding a Knee Joint Stiffness Matrix: A Feasibility Study

The use of multi-body optimisation (MBO) to estimate joint kinematics from stereophotogrammetric data while compensating for soft tissue artefact is still open to debate. Presently used joint models embedded in MBO, such as mechanical linkages, constitute a considerable simplification of joint function, preventing a detailed understanding of it. The present study proposes a knee joint model where femur and tibia are represented as rigid bodies connected through an elastic element the behaviour of which is described by a single stiffness matrix. The deformation energy, computed from the stiffness matrix and joint angles and displacements, is minimised within the MBO. Implemented as a “soft” constraint using a penalty-based method, this elastic joint description challenges the strictness of “hard” constraints. In this study, estimates of knee kinematics obtained using MBO embedding four different knee joint models (i.e., no constraints, spherical joint, parallel mechanism, and elastic joint) were compared against reference kinematics measured using bi-planar fluoroscopy on two healthy subjects ascending stairs. Bland-Altman analysis and sensitivity analysis investigating the influence of variations in the stiffness matrix terms on the estimated kinematics substantiate the conclusions. The difference between the reference knee joint angles and displacements and the corresponding estimates obtained using MBO embedding the stiffness matrix showed an average bias and standard deviation for kinematics of 0.9±3.2° and 1.6±2.3 mm. These values were lower than when no joint constraints (1.1±3.8°, 2.4±4.1 mm) or a parallel mechanism (7.7±3.6°, 1.6±1.7 mm) were used and were comparable to the values obtained with a spherical joint (1.0±3.2°, 1.3±1.9 mm). The study demonstrated the feasibility of substituting an elastic joint for more classic joint constraints in MBO

Characterization of honeys from west and south Buenos Aires province, Argentina

Pollen analyses were carried out on 33 honey samples from Espinal, Monte de Llanuras y Mesetas and Pampeana phytogeographical Provinces, collected during the 2000-2001 period. Sample processing as well as qualitative and quantitative analyses were performed according to standard techniques. Sixty-seven morphological pollen types were identified. The association of Eucalyptus sp. (E. camaldulensis Dehnh., E. viminalis Labill.), Centaurea sp. (C. solstitialis L., C. calcitrapa L.) and Diplotaxis tenuifolia DC. characterized these honeys. Twelve samples were unifloral: six from Eucalyptus sp., five from Helianthus annuus L., and one from Brassicaceae. Asteraceae and Fabaceae were the most representative botanical families

A Melissopalynological map of the south and southwest of the Buenos Aires province, Argentina

El objetivo de este trabajo fue elaborar un mapa melitopalinológico del sur y sudoeste de la provincia de Buenos Aires, Argentina, con datos de análisis polínicos de 127 muestras de miel provenientes de las eco-regiones Pampa, Distrito del Caldén en el Espinal, y Monte de Llanuras y Mesetas, recolectadas en el período 1992-2002. Utilizando análisis de componentes principales y de cluster, los partidos se agruparon en cinco regiones: I (Tres Arroyos, San Cayetano, Coronel Pringles y Coronel Dorrego), II (Guaminí, Saavedra, Coronel Suárez y Adolfo Alsina), III (Coronel Rosales, Monte Hermoso, Bahía Blanca y Villarino), IV (Patagones y Tornquist) y V (Puán). En las Regiones I, III y IV el 80% de las muestras fueron monoflorales. La Región I se caracterizó por la presencia de un 50% de mieles de Helianthus annuus y de un 10% de mieles de trébol; la Región III por 60% de mieles de Eucalyptus sp.; y la Región IV por 30% de mieles de Diplotaxis tenuifolia. En las Regiones II y V el 50% de las mieles fueron monoflorales: la Región II se distinguió por la presencia de 50% de mieles de H. annuus y la Región V por 15% de mieles de Larrea divaricata y 15% de mieles de Vicia sp. Las mieles multiflorales de la Región V se destacaron por la presencia de pólen de Condalia microphylla. La mayor diversidad de tipos polínicos correspondió a las familias Fabaceae y Asteraceae. La asociación de Eucalyptus sp., Centaurea sp. y Diplotaxis tenuifolia caracterizó a las mieles de las cinco regiones. La variabilidad natural de las muestras de miel hace muy difícil definir límites precisos entre las diferentes regiones.The aim of this work was to produce a melissopalynological map of the south and southwest of the Buenos Aires Province, Argentina, using pollen analysis data pertaining to 127 honey samples from the Pampa, Espinal (the Calden District), and Monte de Llanuras y Mesetas ecoregions, collected over the period 1992-2002. Using principal components and hierarchical cluster analysis, the different districts were grouped into five regions: I (Tres Arroyos, San Cayetano, Coronel Pringles and Coronel Dorrego), II (Guaminí, Saavedra, Coronel Suárez and Adolfo Alsina), III (Coronel Rosales, Monte Hermoso, Bahía Blanca and Villarino), IV (Patagones and Tornquist), and V (Puán). In Regions I, III and IV, 80% of honey samples were monofloral: Region I was characterized by the presence of 50% Helianthus annuus honeys and 10% clover honeys, Region III by 65% Eucalyptus sp. honeys, and Region IV by 30% Diplotaxis tenuifolia honeys. In Regions II and V, 50% of honeys were monofloral. Region II was distinguished by the presence of 50% H. annuus honeys, and Region V by 15% Larrea divaricata and 15% Vicia sp. honeys. The multifloral honeys of Region V included samples containing Condalia microphylla pollen. The families Fabaceae and Asteraceae provided the greatest diversity of pollen types. The association of Eucalyptus sp., Centaurea sp., and Diplotaxis tenuifolia characterised the honeys from all five regions. The natural variability of honey samples renders it very difficult to define the boundaries between the different regions

Pollen and nectar sources used by honeybee colonies pollinating sunflower (Helianthus annuus) in the Colorado River Valley, Argentina

Pollen traps and beeswax foundations were set in order to study pollen and nectar sources used by honeybee colonies pollinating sunflower (Helianthus annuus L.) for the production of hybrid seed in the lower valley of the Colorado river in southern Argentina. Thirty-seven plant species in bloom were registered in the area surrounding the sunflower field. Most of them were scarcely represented. The honeybee maximum density recorded on the sunflower male-fertile line was 10.00 bees per 100 heads, while on the male-sterile line was 25.33 bees per 100 heads. Seven plant taxa were identified in the pollen loads and 26 in the honey samples. About 84% of the collected pollen came from three taxa: Centaurea solstitialis L., Eucalyptus camaldulensis Dehnh. and Cirsium vulgare (Savi) Airy -Shaw, and only 11% came from H. annuus. The taxa most gathered had high protein values, above 20%. The dominant and secondary pollen types in honey samples were Tamarix gallica L., E. camaldulensis, Brassicaceae and C. solstitialis. The results indicate that honeybees (Apis mellifera L.) foraged pollen and nectar mainly from the flora surrounding the sunflower field.Fuentes de polen y néctar utilizadas por colonias de Apis mellifera que polinizan girasol (Helianthus annuus) en el valle inferior del río Colorado, Argentina. Se colocaron trampas caza-polen y cuadros con cera estampada para estudiar las fuentes de polen y néctar utilizadas por colmenas que polinizan girasol para la producción de semilla híbrida en el valle inferior del río Colorado, Argentina. Treinta y siete especies en floración fueron registradas en cercanías al cultivo de girasol. La mayoría de ellas estuvieron escasamente representadas. La máxima densidad de abejas en la línea androfértil de girasol fue de 10,00 abejas por cada 100 inflorescencias, mientras que en la línea androestéril fue de 25,33 abejas por cada 100 inflorescencias. En las cargas polínicas se identificaron siete taxa, y 26 en las muestras de miel. El 84% en peso del polen recolectado correspondió a tres taxa: Centaurea solstitialis L., Eucalyptus camaldulensis Dehnh. y Cirsium vulgare (Savi) Airy -Shaw y sólo el 11% provino de H. annuus. Los taxa más cosecha-dos tuvieron valores de proteína altos, superiores a 20%. Los tipos polínicos dominantes y secun-darios en las muestras de miel fueron Tamarix gallica L. y E. camaldulensis, Brassicaceae y C. solstitialis. Los resultados indican que las abejas (Apis mellifera L.) recolectaron polen y néctar principalmente de la flora circundante al cultivo de girasol

Modeling the human tibio-femoral joint using ex vivo determined compliance matrices.

Several approaches have been used to devise a model of the human tibio-femoral joint for embedment in lower limb musculoskeletal models. However, no study has considered the use of cadaveric 6x6 compliance (or stiffness) matrices to model the tibio-femoral joint under normal or pathological conditions. The aim of this paper is to present a method to determine the compliance matrix of an ex vivo tibio-femoral joint for any given equilibrium pose. Experiments were carried out on a single ex vivo knee, first intact and, then, with the anterior cruciate ligament (ACL) transected. Controlled linear and angular displacements were imposed in single degree-of-freedom (DoF) tests to the specimen and resulting forces and moments measured using an instrumented robotic arm. This was done starting from seven equilibrium poses characterized by the following flexion angles: 0°, 15°, 30°, 45°, 60°, 75°and 90°. A compliance matrix for each of the selected equilibrium poses and for both the intact and ACL deficient specimen was calculated. The matrix, embedding the experimental load-displacement relationship of the examined DoFs, was calculated using a linear least squares inversion based on a QR decomposition, assuming symmetric and positive-defined matrices. Single compliance matrix terms were in agreement with the literature. Results showed an overall increase of the compliance matrix terms due to the ACL transection (2.6 ratio for rotational terms at full extension) confirming its role in the joint stabilization. Validation experiments were carried out by performing a Lachman test (the tibia is pulled forward) under load control on both the intact and ACL-deficient knee and assessing the difference (error) between measured linear and angular displacements and those estimated using the appropriate compliance matrix. This error increased non-linearly with respect to the values of the load. In particular, when an incremental posterior-anterior force up to 6 N was applied to the tibia of the intact specimen, the errors on the estimated linear and angular displacements were up to 0.6 mm and 1.5°, while for a force up to 18 N the errors were 1.5 mm and 10.5°, respectively. In conclusion, the method used in this study may be a viable alternative to characterize the tibio-femoral load-dependent behavior in several applications

Observation of the Cabibbo-suppressed decay Xi_c+ -> p K- pi+

We report the first observation of the Cabibbo-suppressed charm baryon decay Xi_c+ -> p K- pi+. We observe 150 +- 22 events for the signal. The data were accumulated using the SELEX spectrometer during the 1996-1997 fixed target run at Fermilab, chiefly from a 600 GeV/c Sigma- beam. The branching fractions of the decay relative to the Cabibbo-favored Xi_c+ -> Sigma+ K- pi+ and Xi_c+ -> X- pi+ pi+ are measured to be B(Xi_c+ -> p K- pi+)/B(Xi_c+ -> Sigma+ K- pi+) = 0.22 +- 0.06 +- 0.03 and B(Xi_c+ -> p K- pi+)/B(Xi_c+ -> X- pi+ pi+) = 0.20 +- 0.04 +- 0.02, respectively.Comment: 5 pages, RevTeX, 3 figures (postscript), Submitted to Phys. Rev. Let