Placental Homing Peptide-microRNA Inhibitor Conjugates For Targeted Enhancement Of Intrinsic Placental Growth Signalling

Suboptimal placental growth and development are the underlying cause of many pregnancy complications. No treatments are available, primarily due to the risk of causing fetal teratogenicity. microRNAs (miRNAs) are short, non-coding RNA sequences that regulate multiple downstream genes; miR-145 and miR675 have previously been identified as negative regulators of placental growth. In this proof of principle study, we explored the feasibility of delivering miRNA inhibitors to the placentas of pregnant mice and developed novel placental homing peptide-microRNA inhibitor conjugates for targeted enhancement of intrinsic placental growth signalling. Scrambled-, miR-145- or miR-675 inhibitor sequences were synthesised from peptide nucleic acids and conjugated to the placental homing peptide CCGKRK. Intravenous administration of the miR-145- and miR-675 conjugates to pregnant C57BL/6J mice significantly increased fetal and placental weights compared to controls; the miR-675 conjugate significantly reduced placental miR-675 expression. When applied to human first trimester placental explants, the miR-145 conjugate significantly reduced placental miR-145 expression, and both conjugates induced significant enhancement of cytotrophoblast proliferation; no effect was observed in term placental explants. This study demonstrates that homing peptide-miRNA inhibitor conjugates can be exploited to promote placental growth; these novel therapeutics may represent an innovative strategy for targeted treatment of compromised placental development

Hubble Space Telescope Imaging of the CFRS and LDSS Redshift Surveys---III. Field elliptical galaxies at 0.2 < z < 1.0

Surface photometry has been performed on a sample of 46 field elliptical galaxies. These galaxies are described well by a deVaucouleurs R^{1/4} profile. The sample was selected from the combined Canada-France and LDSS redshift surveys and spans the range 0.20 < z < 1.00. The relationship between galaxy half-light radius and luminosity evolves such that a galaxy of a given size is more luminous by Delta M_B=-0.97 \pm 0.14 mag at z=0.92 and the mean rest-frame color shifts blueward by Delta (U-V) =-0.68 \pm 0.11 at z=0.92 relative to the local cluster relations. Approximately 1/3 of these elliptical galaxies exhibit [OII] 3727 emission lines with equivalent widths > 15 angstroms indicating ongoing star formation. Estimated star-formation rates imply that \le 5% of the stellar mass in the elliptical galaxy population has been formed since z=1. We see no evidence for a decline in the space density of early-type galaxies with look-back time. The statistics and a comparison with local luminosity functions are both consistent with the view that the population of massive early-type galaxies was largely in place by z~1. This implies that merging is not required since that time to produce the present-day space density of elliptical galaxies.Comment: 21 pages plus 8 figures plus 5 tables. Accepted by Astrophysical Journa

Dynamical versus Stellar Masses of Ultracompact Dwarf Galaxies in the Fornax Cluster

The origin of ultracompact dwarf (UCD) galaxies, compact extragalactic stellar systems, is still a puzzle for present galaxy formation models. We present the comprehensive analysis of high resolution multi-object spectroscopic data for a sample of 24 Fornax cluster UCDs obtained with VLT FLAMES. It comprises previously published data for 19 objects (Mieske et al. 2008) which we re-analysed, including 13 with available HST photometric data. Using Virtual Observatory technologies we found archival HST images for two more UCDs and then determined their structural properties. For all objects we derived internal velocity dispersions, stellar population parameters, and stellar mass-to-light ratios (M/L)* by fitting individual simple stellar population (SSP) synthetic spectra convolved with a Gaussian against the observed spectra using the NBursts full spectral fitting technique. For 14 objects we estimated dynamical masses suggesting no dark matter (DM) in 12 of them and no more than 40 per cent DM mass fraction in the remaining two, in contrast to findings for several UCDs in the Virgo cluster. Some Fornax UCDs even have too high values of (M/L)* estimated using the Kroupa stellar initial mass function (IMF) resulting in negative formally computed DM mass fractions. The objects with too high (M/L)* ratios compared to the dynamical ones have relatively short dynamical relaxation timescales, close to the Hubble time or below. We therefore suggest that their lower dynamical ratios (M/L)dyn are caused by low-mass star depletion due to dynamical evolution. Overall, the observed UCD characteristics suggest at least two formation channels: tidal threshing of nucleated dwarf galaxies for massive UCDs (~10^8 M_sun), and a classical scenario of red globular cluster formation for lower-mass UCDs (< 10^7 M_sun).Comment: Accepted for publication in MNRAS; 13 pages, 9 figures, 2 table

Formation and evolution of dwarf early-type galaxies in the Virgo cluster I. Internal kinematics

We present new medium resolution kinematic data for a sample of 21 dwarf early-type galaxies (dEs) mainly in the Virgo cluster, obtained with the WHT and INT telescopes at the Roque de los Muchachos Observatory (La Palma, Spain). These data are used to study the origin of the dwarf elliptical galaxy population inhabiting clusters. We confirm that dEs are not dark matter dominated galaxies, at least up to the half-light radius. We also find that the observed galaxies in the outer parts of the cluster are mostly rotationally supported systems with disky morphological shapes. Rotationally supported dEs have rotation curves similar to those of star forming galaxies of similar luminosity and follow the Tully-Fisher relation. This is expected if dE galaxies are the descendant of low luminosity star forming systems which recently entered the cluster environment and lost their gas due to a ram pressure stripping event, quenching their star formation activity and transforming into quiescent systems, but conserving their angular momentum.Comment: 24 pages, 15 figures and 7 tables. Replaced to match the journal versio

Integrated single- and two-photon light sheet microscopy using accelerating beams

The authors thank the UK Engineering and Physical Sciences Research Council (grant EP/J01771/X) and the European Union project FAMOS (FP7 ICT, contract no. 317744) for funding. P.P. acknowledges funding by the Estonian Research Council (grants PUTJD8 and PUT369).We demonstrate the first light sheet microscope using propagation invariant, accelerating Airy beams that operates both in single- and two-photon modes. The use of the Airy beam permits us to develop an ultra compact, high resolution light sheet system without beam scanning. In two-photon mode, an increase in the field of view over the use of a standard Gaussian beam by a factor of six is demonstrated. This implementation for light sheet microscopy opens up new possibilities across a wide range of biomedical applications, especially for the study of neuronal processes.Publisher PDFPeer reviewe

Geometrical tests of cosmological models. III. The cosmology-evolution diagram at z=1

The rotational velocity of distant galaxies, when interpreted as a size (luminosity) indicator, may be used as a tool to select high redshift standard rods (candles) and probe world models and galaxy evolution via the classical angular diameter-redshift or Hubble diagram tests. We implement the proposed testing strategy using a sample of 30 rotators spanning the redshift range 0.2<z<1 with high resolution spectra and images obtained by the VIMOS/VLT Deep Redshift Survey (VVDS) and the Great Observatories Origins Deep Survey (GOODs). We show that by applying at the same time the angular diameter-redshift and Hubble diagrams to the same sample of objects (i.e. velocity selected galactic discs) one can derive a characteristic chart, the cosmology-evolution diagram, mapping the relation between global cosmological parameters and local structural parameters of discs such as size and luminosity. This chart allows to put constraints on cosmological parameters when general prior information about discs evolution is available. In particular, by assuming that equally rotating large discs cannot be less luminous at z=1 than at present (M(z=1) < M(0)), we find that a flat matter dominated cosmology (Omega_m=1) is excluded at a confidence level of 2sigma and an open cosmology with low mass density (Omega_m = 0.3) and no dark energy contribution is excluded at a confidence level greater than 1 sigma. Inversely, by assuming prior knowledge about the cosmological model, the cosmology-evolution diagram can be used to gain useful insights about the redshift evolution of the structural parameters of baryonic discs hosted in dark matter halos of nearly equal masses.Comment: 14 pages and 11 figures. A&A in pres

The UV colours of high-redshift early-type galaxies: evidence for recent star formation and stellar mass assembly over the last 8 billion years

We combine deep UBVRIzJK photometry from the Multiwavelength Survey by Yale-Chile (MUSYC) with redshifts from the COMBO-17 survey to perform a large-scale study of the rest-frame ultraviolet (UV) properties of 674 high-redshift (0.5<z<1) early-type galaxies, drawn from the Extended Chandra Deep Field South (E-CDFS). Galaxy morphologies are determined through visual inspection of Hubble Space Telescope (HST) images taken from the GEMS survey. We harness the sensitivity of the UV to young (<1 Gyr old) stars to quantify the recent star formation history of early-type galaxies across a range of luminosities (-23.5 < M(V) < -18). Comparisons to simple stellar populations forming at high redshift indicate that only ~1.1 percent of early-types in this sample are consistent with purely passive ageing since z=2. Parametrising the recent star formation (RSF) in terms of the mass fraction of stars less than a Gyr old, we find that the early-type population as a whole shows a typical RSF between 5 and 13% in the redshift range 0.5<z<1. Early-types on the UV red sequence show RSF values less than 5% while the reddest early-types are virtually quiescent with RSF values of ~1%. We find compelling evidence that early-types of all luminosities form stars over the lifetime of the Universe, although the bulk of their star formation is already complete at high redshift. This tail-end of star formation is measurable and not negligible, with luminous (-23<M(V)<-20.5) early-types potentially forming 10-15% of their mass since z=1, with their less luminous (M(V)>-20.5) counterparts potentially forming 30-60 percent of their mass in the same redshift range. (abridged)Comment: Submitted to MNRA

SPIDER VII - Revealing the Stellar Population Content of Massive Early-type Galaxies out to 8Re

Radial trends of stellar populations in galaxies provide a valuable tool to understand the mechanisms of galaxy growth. In this paper, we present the first comprehensive analysis of optical-optical and optical-NIR colours, as a function of galaxy mass, out to the halo region (8Re) of early-type galaxies (ETGs). We select a sample of 674 massive ETGs (M*>3x10^10MSun) from the SDSS-based SPIDER survey. By comparing with a large range of population synthesis models, we derive robust constraints on the radial trends in age and metallicity. Metallicity is unambiguously found to decrease outwards, with a measurable steepening of the slope in the outer regions (Re<R<8Re). The gradients in stellar age are found to be more sensitive to the models used, but in general, the outer regions of ETGs feature older populations compared to the cores. This trend is strongest for the most massive galaxies in our sample (M*>10^11MSun). Furthermore, when segregating with respect to large scale environment, the age gradient is more significant in ETGs residing in higher density regions. These results shed light on the processes leading from the formation of the central core to the growth of the stellar envelope of massive galaxies. The fact that the populations in the outer regions are older and more metal-poor than in the core suggests a process whereby the envelope of massive galaxies is made up of accreted small satellites (i.e. minor mergers) whose stars were born during the first stages of galaxy formation.Comment: 20 pages, 13 figures, 10 tables. Accepted for publication in MNRA

Building galaxies by accretion and in-situ star formation

We examine galaxy formation in a cosmological AMR simulation, which includes two high resolution boxes, one centered on a 3 \times 10^14 M\odot cluster, and one centered on a void. We examine the evolution of 611 massive (M\ast > 10^10M\odot) galaxies. We find that the fraction of the final stellar mass which is accreted from other galaxies is between 15 and 40% and increases with stellar mass. The accreted fraction does not depend strongly on environment at a given stellar mass, but the galaxies in groups and cluster environments are older and underwent mergers earlier than galaxies in lower density environments. On average, the accreted stars are ~2.5 Gyrs older, and ~0.15 dex more metal poor than the stars formed in-situ. Accreted stellar material typically lies on the outskirts of galaxies; the average half-light radius of the accreted stars is 2.6 times larger than that of the in-situ stars. This leads to radial gradients in age and metallicity for massive galaxies, in qualitative agreement with observations. Massive galaxies grow by mergers at a rate of approximately 2.6% per Gyr. These mergers have a median (mass-weighted) mass ratio less than 0.26 \pm 0.21, with an absolute lower limit of 0.20, for galaxies with M\ast ~ 10^12 M\odot. This suggests that major mergers do not dominate in the accretion history of massive galaxies. All of these results agree qualitatively with results from SPH simulations by Oser et al. (2010, 2012).Comment: 18 pages, 12 figures, submitted to MNRA

Insider trading and CEO pay-gap induced turnover

We explore how insider trading returns, disparities in executive pay, and CEO turnover are interrelated. Our findings reveal both independent and interactive effects for insider trading returns, the CEO pay gap, and the likelihood of CEO turnover. First, an increase in abnormal returns from insider purchases lowers the probability of a CEO’s turnover, while an increase in abnormal returns from insider sales increases the likelihood of a CEO’s dismissal. Second, the CEO pay gap negatively affects the probability of CEO turnover for insider purchases, but it does not have a similar effect on insider sales. Third, the interaction between insider abnormal returns and any CEO pay disparity influences the impact of these returns on CEO turnover. Specifically, this interaction diminishes the positive effect of insider selling on the probability of a CEO’s dismissal, offsets the negative effect of insider purchasing on CEO dismissal, and, finally, amplifies the negative impact of CEO pay disparity on the probability of a CEO’s dismissal during periods witnessing insider purchases