Protective effect of carboxymethyl-glucan (CM-G) against DNA damage in patients with advanced prostate cancer

Carboxymethyl-glucan (CM-G) is a soluble derivative from Saccharomyces cerevisiae (1 → 3)(1 → 6)-β-D-glucan. The protective efficiency of CM-G against DNA damage in cells from patients with advanced prostate cancer (PCa), and undergoing Androgen Deprivation Therapy (ADT), was evaluated. DNA damage scores were obtained by the comet assay, both before and after treatment with CM-G. The reduction in DNA damage, ranging from 18% to 87%, with an average of 59%, was not related to the increased number of leukocytes in peripheral blood. The results demonstrate for the first time the protective effect of CM-G against DNA damage in patients with advanced PCa. Among smokers, three presented the highest reduction in DNA damage after treatment with CM-G. There was no observable relationship between DNA damage scores before and after treatment, and age, alcoholism and radiotherapy

Estudo da poluição pontual e difusa na bacia de contribuição do reservatório da usina hidrelétrica de Funil utilizando modelagem espacialmente distribuída em Sistema de Informação Geográfica

RESUMO Este estudo avaliou o potencial poluidor da bacia de contribuição do reservatório de Funil (BCRF), localizado na bacia hidrográfica do rio Paraíba do Sul, considerando a geração da carga de nutrientes, nitrogênio (N) e fósforo (P), por fontes pontuais e difusas, a partir de uma modelagem distribuída utilizando Sistema de Informação Geográfica (SIG). As cargas e concentrações médias anuais desses nutrientes foram geradas a partir do acoplamento de equações empíricas, em SIG, considerando informações espaciais de uso e cobertura do solo, população residente na bacia e vazão média anual de longo período, obtida por equações do tipo chuva vazão. Os resultados indicaram que 80% da carga total de nitrogênio foram provenientes de fontes pontuais e 20% de fontes difusas, enquanto que, da carga total de fósforo, 89,1% foram originadas de fontes pontuais e 10,9% de fontes difusas. As concentrações de nutrientes estimadas pelo modelo empírico apresentaram bons ajustes em relação aos valores observados de fósforo e de nitrogênio no rio Paraíba do Sul, com R²=0,96 (p<0,01) e R²=0,70 (p<0,01), respectivamente. Dessa forma, o modelo foi capaz de detectar, de forma significativa, a tendência das variações nas concentrações de nutrientes ao longo de diferentes trechos da BCRF

2023 International Consensus Meeting on Musculoskeletal Infection:Summary from the Treatment Workgroup and Consensus on Treatment in Preclinical Models

In vitro and in vivo studies are critical for the preclinical efficacy assessment of novel therapies targeting musculoskeletal infections (MSKI). Many preclinical models have been developed and applied as a prelude to evaluating safety and efficacy in human clinical trials. In performing these studies, there is both a requirement for a robust assessment of efficacy, as well as a parallel responsibility to consider the burden on experimental animals used in such studies. Since MSKI is a broad term encompassing infections varying in pathogen, anatomical location and implants used, there are also a wide range of animal models described modelling these disparate infections. Although some of these variations are required to adequately evaluate specific interventions, there would be enormous value in creating a unified and standardized criteria to animal testing in the treatment of MSKI. The Treatment Workgroup of the 2023 International Consensus Meeting on Musculoskeletal Infection was responsible for questions related to preclinical models for treatment of MSKI. The main objective was to review the literature related to priority questions and estimate consensus opinion after voting. This document presents that process and results for preclinical models related to 1) animal model considerations, 2) outcome measurements, and 3) imaging. This article is protected by copyright. All rights reserved