1,292 research outputs found
Avaliacoes da infeccao de explantes de feijoeiro por Agrobacterium tumefaciens por meio de microscopia.
Phaseolus vulgaris L. e considerada recalcitrante a transformacao por Agrobacterium tumefaciens. Contudo, alteracoes no meio de co-cultivo, utilizacao de linhagens hipervirulentas de Agrobacterium e de vetores binarios contendo genes vir demostraram que o feijoeiro e susceptivel a essa bacteria. O objetivo do presente trabalho foi estudar o efeito da sonificacao nos tecidos vegetais de feijoeiro, bem como a penetracao da Agrobacterium nas camadas subepidermicas do tecido vegetal, usando a metodologia SAAT ("Sonification-Assisted Agrobacterium-mediated Transformation"). A variedade de feijoeiro utilizada foi a Olathe Pinto, a linhagem de A. tumefaciens foi LBA4404:pTOK.Os embrioes de feijao foram pre-tratados po 14 dias em meio de multibrotacao e, entao submetidos a sonificacao (de 0 ou 60 segundos) na presenca de Agrobacterium. Apos a inoculacao foram co-cultivados por 24 horas em meio liquido seguido de 48 horas em meio solido, ambos, contendo 20 m. L -1 de acetoceringona. Os explantes inoculados foram fixadas em solucao de Karnovsk para avaliacoes em microscopia optica e eletronica de varredura. As analises da microspia demostraram a presenca de rupturas na epiderme, quebras da parede celular e invasao da Agrobacterium nos tecidos subepidermicos. Os reultados demostraram que o metodo SAAT e uma tecnica viavel para a inoculcao de Agrobacterium em explantes de P. vulgaris.bitstream/CPAC-2010/23710/1/bolpd-04.pd
Self-consistent simulation of plasma scenarios for ITER using a combination of 1.5D transport codes and free-boundary equilibrium codes
Self-consistent transport simulation of ITER scenarios is a very important
tool for the exploration of the operational space and for scenario
optimisation. It also provides an assessment of the compatibility of developed
scenarios (which include fast transient events) with machine constraints, in
particular with the poloidal field (PF) coil system, heating and current drive
(H&CD), fuelling and particle and energy exhaust systems. This paper discusses
results of predictive modelling of all reference ITER scenarios and variants
using two suite of linked transport and equilibrium codes. The first suite
consisting of the 1.5D core/2D SOL code JINTRAC [1] and the free boundary
equilibrium evolution code CREATE-NL [2,3], was mainly used to simulate the
inductive D-T reference Scenario-2 with fusion gain Q=10 and its variants in H,
D and He (including ITER scenarios with reduced current and toroidal field).
The second suite of codes was used mainly for the modelling of hybrid and
steady state ITER scenarios. It combines the 1.5D core transport code CRONOS
[4] and the free boundary equilibrium evolution code DINA-CH [5].Comment: 23 pages, 18 figure
Telomere length shortening is associated with treatment-free remission in chronic myeloid leukemia patients
We studied telomere length in 32 CML patients who discontinued imatinib after achieving complete molecular remission and 32 age-sex-matched controls. The relative telomere length (RTL) was determined by q-PCR as the telomere to single copy gene (36B4) ratio normalized to a reference sample (K-562 DNA). Age-corrected RTL (acRTL) was also obtained. The 36-month probability of treatment-free remission (TFR) was 59.4 %. TFR patients showed shorter acRTL compared to relapsed (mean ± SD = 0.01 ± 0.14 vs 0.20 ± 0.21; p = 0.01). TFR was significantly higher in CML patients with acRTL ≤0.09 (78.9 vs 30.8 %, p = 0.002). CML stem cells harboring longer telomeres possibly maintain a proliferative potential after treatment discontinuation
Acute pancreatitis in children. An Italian multicentre study
AIM:
To evaluate the clinical, morphological and aetiological aspects of acute pancreatitis in children in Italy.
PATIENTS:
The hospital records of 50 consecutive patients with acute pancreatitis observed in 5 Italian Pediatric Departments were reviewed.
RESULTS:
A total of 25 males and 25 females (median age 10.5 years, range 2-17) were studied. Of these patients, 48 (96%) had abdominal pain. The pancreatitis was associated with biliary disease in 10 patients (20%); it was due to viral infection in 6 patients (12%), pancreatic duct abnormalities in 4 (8%, familial chronic pancreatitis in 3 (6%), trauma in 5 (10%) and other causes in 5 (10%); the pancreatitis was of unknown origin in 17 patients (34%). Previous attacks of the disease had occurred in 14 patients. A diagnosis of mild pancreatitis was made in 41 patients (82%) and of severe disease in 9 (18%). One patient with severe pancreatitis died from multiorgan failure. Patients with severe pancreatitis had significantly higher serum concentrations of C-reactive protein than patients with mild pancreatitis. Hospital stay was similar for patients with the mild form and those with the severe form of the disease.
CONCLUSIONS:
In Italian children, acute pancreatitis is of unknown origin in about one-third of the children and is recurrent in 28% of the cases. The disease is severe in 18% of the case
Evidence of resonant surface wave excitation in the relativistic regime through measurements of proton acceleration from grating targets
The interaction of laser pulses with thin grating targets, having a periodic
groove at the irradiated surface, has been experimentally investigated.
Ultrahigh contrast () pulses allowed to demonstrate an enhanced
laser-target coupling for the first time in the relativistic regime of
ultra-high intensity >10^{19} \mbox{W/cm}^{2}. A maximum increase by a factor
of 2.5 of the cut-off energy of protons produced by Target Normal Sheath
Acceleration has been observed with respect to plane targets, around the
incidence angle expected for resonant excitation of surface waves. A
significant enhancement is also observed for small angles of incidence, out of
resonance.Comment: 5 pages, 5 figures, 2nd version implements final correction
Test of candidate light distributors for the muon (g2) laser calibration system
The new muon (g-2) experiment E989 at Fermilab will be equipped with a laser
calibration system for all the 1296 channels of the calorimeters. An
integrating sphere and an alternative system based on an engineered diffuser
have been considered as possible light distributors for the experiment. We
present here a detailed comparison of the two based on temporal response,
spatial uniformity, transmittance and time stability.Comment: accepted to Nucl.Instrum.Meth.
Study of shock waves generation, hot electron production and role of parametric instabilities in an intensity regime relevant for the shock ignition
We present experimental results at intensities relevant to Shock Ignition
obtained at the sub-ns Prague Asterix Laser System in 2012 . We studied shock waves
produced by laser-matter interaction in presence of a pre-plasma. We used a first beam at
1ω (1315 nm) at 7 × 10 13 W/cm 2 to create a pre-plasma on the front side of the target and
a second at 3ω (438 nm) at ∼ 10 16 W/cm 2 to create the shock wave. Multilayer targets
composed of 25 (or 40 μm) of plastic (doped with Cl), 5 μm of Cu (for Kα diagnostics)
and 20 μm of Al for shock measurement were used. We used X-ray spectroscopy of Cl
to evaluate the plasma temperature, Kα imaging and spectroscopy to evaluate spatial and
spectral properties of the fast electrons and a streak camera for shock breakout measurements.
Parametric instabilities (Stimulated Raman Scattering, Stimulated Brillouin Scattering and
Two Plasmon Decay) were studied by collecting the back scattered light and analysing its
spectrum. Back scattered energy was measured with calorimeters. To evaluate the maximum
pressure reached in our experiment we performed hydro simulations with CHIC and DUED
codes. The maximum shock pressure generated in our experiment at the front side of the
target during laser-interaction is 90 Mbar. The conversion efficiency into hot electrons was
estimated to be of the order of ∼ 0.1% and their mean energy in the order ∼50 keV.
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