Morphological and population aspects of Diabrotica speciosa speciosa (Germar) (Coleoptera: Chrysomelidae) under laboratory conditions

Diabrotica speciosa speciosa (Germar) es uno de los crisomélidos más ampliamente distribuídos en cultivos de la Argentina. Se estudió la morfología de los estados preimaginales, tabla de vida y el comportamiento reproductivo. El desarrollo se llevó a cabo bajo condiciones controladas de laboratorio. Bajo estas condiciones se establecieron los siguientes parámetros poblacionales: tiempo de desarrollo, tasa reproductiva, tasa de crecimiento intrínseco y se confeccionó una tabla de vida horizontal.Diabrotica speciosa speciosa (Germar) is one of the most widely distributed chrysomelid in crops in Argentina. The morphology of preimaginal stages, life span and reproductive behavior were studied. The development were carried out under controlled conditions. Under these conditions the following population parameters were computed: generation time, reproductive rate, intrinsic rate of increase and an horizontal life table was made.Fil: Defagó, María Teresa. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Investigaciones Entomológicas de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cabrera, Nora Clara. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Departamento Científico de Entomologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Laguzzi, Stella M.. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Investigaciones Entomológicas de Córdoba; ArgentinaFil: Novara, Claudia R.. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Investigaciones Entomológicas de Córdoba; Argentin

On the separation of regularity properties of the reals

We present a model where omega (1) is inaccessible by reals, Silver measurability holds for all sets but Miller and Lebesgue measurability fail for some sets. This contributes to a line of research started by Shelah in the 1980s and more recently continued by Schrittesser and Friedman (see [7]), regarding the separation of different notions of regularity properties of the real line

Human involucrin promoter mediates repression-resistant and compartment-specific LEKTI expression

Gene-modified skin grafts, produced through gene transfer to human keratinocyte stem cells, offer the possibility of therapeutic benefit for inherited skin diseases. We have previously described efficient lentiviral vector–mediated gene transfer to keratinocyte stem cells and the generation of human skin grafts for the inherited skin disease, Netherton syndrome, which arises due to mutations in serine protease inhibitor Kazal-type 5 (SPINK5). Vectors incorporating an internal murine retroviral–derived promoter [spleen focus-forming virus (SFFV)] in combination with a codon-optimized SPINK5 transgene supported high levels of reconstitution and robust correction of skin architecture. Subsequent longer-term experiments have uncovered unanticipated silencing phenomena, with loss of SPINK5 gene expression over time. The inadvertent introduction of CpG sites during codon optimization appears to have rendered vectors susceptible to silencing due to methylation across the promoter–transgene boundary. Substitution of the methylation-susceptible SFFV promoter with a 572-bp minimal human involucrin promoter (INVOp), which encodes very few CpG sites, prevented repression of the SPINK5 transgene and resulted in durable and highly compartment-specific reconstitution of lympho-epithelial Kazal-type–related inhibitor (LEKTI) in human skin grafted onto immunodeficient mice.We conclude that skin grafts modified with lentiviral vectors encoding INVOp offer a suitable platform for therapeutic gene therapy in Netherton syndrome, and our experience highlights unanticipated effects of transgene codon optimization